PPM1F Recombinant Protein

Name: PPM1F Recombinant Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS205068
Price: 230 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS205068

PPM1F is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block… More details

$230 / 0.02 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

PPM1F, Recombinant Protein

Full Product Name

PPM1F, 1-454aa, Human, His tag, E Coli

Product Gene Name

PPM1F recombinant protein

Product Synonym Names

Protein phosphatase 1F; CAMKP; CaMKPase; FEM-2; hFEM-2; POPX2

Sequence

MGSSHHHHHH SSGLVPRGSH MGSMSSGAPQ KSSPMASGAE ETPGFLDTLL QDFPALLNPE DPLPWKAPGT VLSQEEVEGE LAELAMGFLG SRKAPPPLAA ALAHEAVSQL LQTDLSEFRK LPREEEEEEE DDDEEEKAPV TLLDAQSLAQ SFFNRLWEVA GQWQKQVPLA ARASQRQWLV SIHAIRNTRR KMEDRHVSLP SFNQLFGLSD PVNRAYFAVF DGHGGVDAAR YAAVHVHTNA ARQPELPTDP EGALREAFRR TDQMFLRKAK RERLQSGTTG VCALIAGATL HVAWLGDSQV ILVQQGQVVK LMEPHRPERQ DEKARIEALG GFVSHMDCWR VNGTLAVSRA IGDVFQKPYV SGEADAASRA LTGSEDYLLL ACDGFFDVVP HQEVVGLVQS HLTRQQGSGL RVAEELVAAA RERGSHDNIT VMVVFLRDPQ ELLEGGNQGE GDPQAEGRRQ DLPSSLPEPE TQAPPRS

Host

E Coli

Form/Format

Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT.

Purity/Purification

> 90% by SDS-PAGE

Concentration

1 mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

52kDa (477aa)

3D Structure

ModBase 3D Structure for P49593

Antigen Species

Human

Tag Information

His-tag

Product Categories/Family for PPM1F recombinant protein

Signal Transduction

Applications Tested/Suitable for PPM1F recombinant protein

SDS-PAGE

Product References and Citations for PPM1F recombinant protein

Tan KM, Chan SL, et al. (2001). J Biol Chem. 276 (47):44193-202. Harvey BP, Banga SS, et al. (2004). J Biol Chem. 279 (23):24889-98.

Other Notes

Small volumes of PPM1F recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PPM1F purchase

MBS205068 is a recombinant protein targeting PPM1F. Also known as PPM1F, 1-454aa, Human, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for PPM1F. It may not necessarily be applicable to this product.

NCBI GI #

7661862

NCBI GeneID

9647

NCBI Accession #

NP_055449

UniProt Primary Accession #

P49593

UniProt Secondary Accession #

Q96PM2; A8K6G3; B7Z2C3

UniProt Related Accession #

P49593

GenBank Nucleotide #

NM_014634.3

NCBI Official Full Name

protein phosphatase 1F

NCBI Official Synonym Full Names

protein phosphatase, Mg2+/Mn2+ dependent 1F

NCBI Official Symbol

PPM1F

NCBI Official Synonym Symbols

CAMKP; FEM-2; POPX2; hFEM-2; CaMKPase

UniProt Protein Name

Protein phosphatase 1F

UniProt Synonym Protein Names

Ca(2+)/calmodulin-dependent protein kinase phosphatase; CaM-kinase phosphatase; CaMKPase; Partner of PIX 2; Protein fem-2 homolog; hFem-2

Protein Family

Protein phosphatase

UniProt Gene Name

PPM1F

UniProt Synonym Gene Names

KIAA0015; POPX2; CaM-kinase phosphatase; CaMKPase; hFem-2

NCBI Protein Information

protein phosphatase 1F

NCBI Summary for PPM1F

The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined. [provided by RefSeq, Jul 2008]

UniProt Comments for PPM1F

Dephosphorylates and concomitantly deactivates CaM-kinase II activated upon autophosphorylation, and CaM-kinases IV and I activated upon phosphorylation by CaM-kinase kinase. Promotes apoptosis.

Research Articles on PPM1F

1. This study, combined with our previous findings, suggests that a single ubiquitously expressed phosphatase POPX2 influences cancer metastasis via modulating multiple biological processes including MAPK signaling and exosome cytokine secretion.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with PPM1F recombinant protein

Disease Name	Pubmed Publications
Breast Neoplasms Antibodies	>4 publications with PPM1F and Breast Neoplasms
Liver Diseases Antibodies	>1 publications with PPM1F and Liver Diseases
Carcinoma, Hepatocellular Antibodies	>1 publications with PPM1F and Carcinoma, Hepatocellular
Adenocarcinoma Antibodies	>1 publications with PPM1F and Adenocarcinoma
Liver Neoplasms Antibodies	>1 publications with PPM1F and Liver Neoplasms

Organs/Tissues associated with PPM1F recombinant protein

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>7 publications with PPM1F and Brain
Blood Antibodies	>1 publications with PPM1F and Blood
Kidney Antibodies	>1 publications with PPM1F and Kidney
Liver Antibodies	>1 publications with PPM1F and Liver