DPPA4 Recombinant Protein

Name: DPPA4 Recombinant Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS205093
Price: 245 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS205093

DPPA4 may be involved in the maintenance of active epigenetic status of target genes and may inhibit differentiation of embryonic cells into a primitive ectoderm lineage. Recombinant human DPPA4 protein, fused to His-tag at N-terminus, was expressed in E Coli… More details

$245 / 0.02 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

DPPA4, Recombinant Protein

Full Product Name

DPPA4, 1-304aa, Human, His tag, E Coli

Product Gene Name

DPPA4 recombinant protein

Product Synonym Names

Developmental pluripotency associated 4; 2410091M23Rik

Sequence

MGSSHHHHHH SSGLVPRGSH MGSMLRGSAS STSMEKAKGK EWTSTEKSRE EDQQASNQPN SIALPGTSAK RTKEKMSIKG SKVLCPKKKA EHTDNPRPQK KIPIPPLPSK LPPVNLIHRD ILRAWCQQLK LSSKGQKLDA YKRLCAFAYP NQKDFPSTAK EAKIRKSLQK KLKVEKGETS LQSSETHPPE VALPPVGEPP ALENSTALLE GVNTVVVTTS APEALLASWA RISARARTPE AVESPQEASG VRWCVVHGKS LPADTDGWVH LQFHAGQAWV PEKQEGRVSA LFLLPASNFP PPHLEDNMLC PKCVHRNKVL IKSLQWE

Host

E Coli

Form/Format

Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Purity/Purification

> 85% by SDS-PAGE

Concentration

0.5 mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

35.9 kDa (327aa), confirmed by MALDI-TOF

OMIM

AK001575 mRNA

3D Structure

ModBase 3D Structure for Q7L190

Antigen Species

Human

Tag Information

His-tag

Product Categories/Family for DPPA4 recombinant protein

Development

Applications Tested/Suitable for DPPA4 recombinant protein

SDS-PAGE

Product References and Citations for DPPA4 recombinant protein

Ota T., et al. (2004) Nat. Genet. 36:40-45; Muzny D.M., et al. (2006) Nature. 440:1194-1198;

Other Notes

Small volumes of DPPA4 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DPPA4 purchase

MBS205093 is a recombinant protein targeting DPPA4. Also known as DPPA4, 1-304aa, Human, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for DPPA4. It may not necessarily be applicable to this product.

NCBI GI #

144953903

NCBI GeneID

55211

NCBI Accession #

NP_060659

UniProt Primary Accession #

Q7L190

UniProt Secondary Accession #

Q9H9N5; Q9NVI6; A8K4M7

UniProt Related Accession #

Q7L190

GenBank Nucleotide #

NM_018189.3

NCBI Official Full Name

developmental pluripotency-associated protein 4 isoform 1

NCBI Official Synonym Full Names

developmental pluripotency associated 4

NCBI Official Symbol

DPPA4

NCBI Official Synonym Symbols

2410091M23Rik

UniProt Protein Name

Developmental pluripotency-associated protein 4

Protein Family

Developmental pluripotency-associated protein

UniProt Gene Name

DPPA4

NCBI Protein Information

developmental pluripotency-associated protein 4

NCBI Summary for DPPA4

This gene encodes a nuclear factor that is involved in the maintenance of pluripotency in stem cells and essential for embryogenesis. The encoded protein has a scaffold-attachment factor A/B, acinus and PIAS (SAP) domain that binds DNA and is thought to modify chromatin. Mice with a homozygous knockout of the orthologous gene die during late embryonic development or within hours after birth. Knockout embryos are normal in size at embryonic day 18.5 but exhibit skeletal and lung tissue abnormalities. This gene, when mutated, is highly expressed in embryonal carcinomas, pluripotent germ cell tumors, and other cancers and is thought to play an important role in tumor progression. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2017]

UniProt Comments for DPPA4

May be involved in the maintenance of active epigenetic status of target genes. May inhibit differentiation of embryonic cells into a primitive ectoderm lineage.

Research Articles on DPPA4

1. knockdown of PCGF1 results in reduced expression of DPPA4 and other subunits of the variant PRC1 complex at both mRNA and protein levels

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with DPPA4 recombinant protein

Reference Product	PubMed Publications
SOX2 recombinant protein	>13 publications with DPPA4 and SOX2
NANOG recombinant protein	>13 publications with DPPA4 and NANOG
POU5F1 recombinant protein	>5 publications with DPPA4 and POU5F1
DNMT3B recombinant protein	>4 publications with DPPA4 and DNMT3B
GDF3 recombinant protein	>2 publications with DPPA4 and GDF3
LIN28A recombinant protein	>2 publications with DPPA4 and LIN28A
AFP recombinant protein	>1 publications with DPPA4 and AFP

Pathways associated with DPPA4 recombinant protein

Products by Pathway	Pathway Diagram
Developmental Biology Pathway antibodies	Developmental Biology Pathway Diagram
POU5F1 (OCT4), SOX2, NANOG Activate Genes Related To Proliferation Pathway antibodies	POU5F1 (OCT4), SOX2, NANOG Activate Genes Related To Proliferation Pathway Diagram
Transcriptional Regulation Of Pluripotent Stem Cells Pathway antibodies	Transcriptional Regulation Of Pluripotent Stem Cells Pathway Diagram

Organs/Tissues associated with DPPA4 recombinant protein

Organ/Tissue Name	Pubmed Publications
Embryonic Tissue Antibodies	>9 publications with DPPA4 and Embryonic Tissue
Testis Antibodies	>6 publications with DPPA4 and Testis
Bone Marrow Antibodies	>3 publications with DPPA4 and Bone Marrow
Placenta Antibodies	>1 publications with DPPA4 and Placenta
Ovary Antibodies	>1 publications with DPPA4 and Ovary