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glpE Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS205141

Thiosulfate:cyanide sulfurtransferase (rhodanese), also known as glpE, is a mitochondrial matrix enzyme that is encoded by the nucleus. glpE catalyzes the sulfur-transfer reaction in which a sulfur atom is transferred from thiosulfate to cyanide by a… More details

$230 / 0.02 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
glpE, Recombinant Protein
Full Product Name
glpE, 1-108aa, E Coli, His tag, E Coli
Product Gene Name
Product Synonym Names
Thiosulfate:cyanide sulfurtransferase (rhodanese); ECK3411; JW3388
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMDQFECI NVADAHQKLQ EKEAVLVDIR DPQSFAMGHA VQAFHLTNDT LGAFMRDNDF DTPVMVMCYH GNSSKGAAQY LLQQGYDVVY SIDGGFEAWQ RQFPAEVAYG A
Host
E Coli
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol
Purity/Purification
> 95% by SDS-PAGE
Concentration
0.5 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
14.5kDa (131aa), confirmed by MALDI-TOF
3D Structure
ModBase 3D Structure for P0A6V5
Antigen Species
E Coli
Tag Information
His-tag

Product Categories/Family for glpE recombinant protein

Applications Tested/Suitable for glpE recombinant protein
SDS-PAGE

Product References and Citations for glpE recombinant protein

Li H., et al. (2011) Biomol NMR Assign. 5 (1):97-9; Ray WK., et al. (2000) J Bacteriol. 182 (8):2277-84.

Other Notes

Small volumes of glpE recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for glpE purchase

MBS205141 is a recombinant protein targeting glpE. Also known as glpE, 1-108aa, E Coli, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for glpE. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
GenBank Nucleotide #
NCBI Official Full Name
thiosulfate:cyanide sulfurtransferase (rhodanese)
NCBI Official Symbol
glpE
NCBI Official Synonym Symbols
ECK3411; JW3388
UniProt Protein Name
Thiosulfate sulfurtransferase GlpE
UniProt Gene Name
glpE
NCBI Protein Information
thiosulfate:cyanide sulfurtransferase (rhodanese)

NCBI Summary for glpE

glpE, yceA and yibN are paralogs. [More information is available at EcoGene: EG10395].

UniProt Comments for glpE

Catalyzes, although with low efficiency, the sulfur transfer reaction from thiosulfate to cyanide. The relatively low affinity of GlpE for both thiosulfate and cyanide suggests that these compounds are not the physiological substrates. Thioredoxin 1 or related dithiol proteins could instead be the physiological sulfur-acceptor substrate. Possible association with the metabolism of glycerol-phosphate remains to be elucidated.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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