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RPS18 Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS205145

40S ribosomal protein S18, also known as RPS18, belongs to the ribosomal protein S13P family. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. RPS18 is a known binding partner of Cofilin and… More details

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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
RPS18, Recombinant Protein
Full Product Name
RPS18, 1-152aa, Human, His tag, E Coli (Denatured)
Product Gene Name
Product Synonym Names
40S ribosomal protein S18; D6S218E; HKE3; KE-3; KE3; S18
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMSLVIPE KFQHILRVLN TNIDGRRKIA FAITAIKGVG RRYAHVVLRK ADIDLTKRAG ELTEDEVERV ITIMQNPRQY KIPDWFLNRQ KDVKDGKYSQ VLANGLDNKL REDLERLKKI RAHRGLRHFW GLRVRGQHTK TTGRRGRTVG VSKKK
Host
E Coli
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.4M urea, 10% glycerol
Purity/Purification
> 90% by SDS-PAGE
Concentration
0.5 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
20.1 kDa (175aa)
OMIM
X69150 mRNA
3D Structure
ModBase 3D Structure for P62269
Antigen Species
Human
Tag Information
His-tag

Product Categories/Family for RPS18 recombinant protein

Applications Tested/Suitable for RPS18 recombinant protein
SDS-PAGE
Application Notes for RPS18 recombinant protein
This protein is denatured. Denatured protein is most likely not the best option for functional studies. Denatured protein is better suited for Western Blot (WB) or imaging assays. Applications are user determined.

Product References and Citations for RPS18 recombinant protein

Chan Y., et al. (1991) Biochem Biophys Res Commun. 178:1212-1218.; Mishra Gorur K., et al. (2002) J Biol Chem. 277:33537-33540.;

Other Notes

Small volumes of RPS18 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RPS18 purchase

MBS205145 is a recombinant protein targeting RPS18. Also known as RPS18, 1-152aa, Human, His tag, E Coli (Denatured). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for RPS18. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
40S ribosomal protein S18
NCBI Official Synonym Full Names
ribosomal protein S18
NCBI Official Symbol
RPS18
NCBI Official Synonym Symbols
KE3; S18; HKE3; KE-3; D6S218E
UniProt Protein Name
40S ribosomal protein S18
UniProt Synonym Protein Names
Ke-3; Ke3; Small ribosomal subunit protein uS13
Protein Family
UniProt Gene Name
RPS18
UniProt Synonym Gene Names
D6S218E; Ke3
NCBI Protein Information
40S ribosomal protein S18

NCBI Summary for RPS18

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S13P family of ribosomal proteins. It is located in the cytoplasm. The gene product of the E. coli ortholog (ribosomal protein S13) is involved in the binding of fMet-tRNA, and thus, in the initiation of translation. This gene is an ortholog of mouse Ke3. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
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UniProt Comments for RPS18

Located at the top of the head of the 40S subunit, it contacts several helices of the 18S rRNA.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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