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HLA-DOA Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS205168

HLA-DOA belongs to the HLA class II alpha chain paralogues. HLA-DOA forms a heterodimer with HLA-DOB. The heterodimer, HLA-DO, is found in lysosomes in B cells and regulates HLA-DM-mediated peptide loading on MHC class II molecules. In comparison with… More details

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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
HLA-DOA, Recombinant Protein
Full Product Name
HLA-DOA, 26-217aa, Human, His tag, E Coli (Denatured)
Product Synonym Names
HLA class II histocompatibility antigen DO alpha chain; HLA class II histocompatibility antigen; DO alpha chain; HLA-DNA; HLA-DZA; HLADZ
Sequence
MGSSHHHHHH SSGLVPRGSH MGSTKADHMG SYGPAFYQSY GASGQFTHEF DEEQLFSVDL KKSEAVWRLP EFGDFARFDP QGGLAGIAAI KAHLDILVER SNRSRAINVP PRVTVLPKSR VELGQPNILI CIVDNIFPPV INITWLRNGQ TVTEGVAQTS FYSQPDHLFR KFHYLPFVPS AEDVYDCQVE HWGLDAPLLR HWELQVPIPP PDAME
Host
E Coli
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.4M urea, 10% glycerol
Purity/Purification
> 90% by SDS-PAGE
Concentration
1 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
24.1 kDa (215aa)
OMIM
X02882 Genomic DNA
3D Structure
ModBase 3D Structure for P06340
Antigen Species
Human
Tag Information
His-tag

Product Categories/Family for HLA-DOA recombinant protein

Applications Tested/Suitable for HLA-DOA recombinant protein
SDS-PAGE
Application Notes for HLA-DOA recombinant protein
This protein is denatured. Denatured protein is most likely not the best option for functional studies. Denatured protein is better suited for Western Blot (WB) or imaging assays. Applications are user determined.

Product References and Citations for HLA-DOA recombinant protein

Yoon,T., et al. (2012) Proc. Natl. Acad. Sci. u.S.A. 109 (28), 11276-11281; Gieger,C., et al. (2012) Nature 480 (7376), 201-208

Other Notes

Small volumes of HLA-DOA recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HLA-DOA purchase

MBS205168 is a recombinant protein targeting HLA-DOA. Also known as HLA-DOA, 26-217aa, Human, His tag, E Coli (Denatured). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for HLA-DOA. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
HLA class II histocompatibility antigen, DO alpha chain
NCBI Official Synonym Full Names
major histocompatibility complex, class II, DO alpha
NCBI Official Symbol
HLA-DOA
NCBI Official Synonym Symbols
HLADZ; HLA-DNA; HLA-DZA
UniProt Protein Name
HLA class II histocompatibility antigen, DO alpha chain
UniProt Synonym Protein Names
MHC DN-alpha; MHC DZ alpha; MHC class II antigen DOA
UniProt Gene Name
HLA-DOA
UniProt Synonym Gene Names
HLA-DNA; HLA-DZA
NCBI Protein Information
HLA class II histocompatibility antigen, DO alpha chain

NCBI Summary for HLA-DOA

HLA-DOA belongs to the HLA class II alpha chain paralogues. HLA-DOA forms a heterodimer with HLA-DOB. The heterodimer, HLA-DO, is found in lysosomes in B cells and regulates HLA-DM-mediated peptide loading on MHC class II molecules. In comparison with classical HLA class II molecules, this gene exhibits very little sequence variation, especially at the protein level. [provided by RefSeq, Jul 2008]
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UniProt Comments for HLA-DOA

Important modulator in the HLA class II restricted antigen presentation pathway by interaction with the HLA-DM molecule in B-cells. Modifies peptide exchange activity of HLA-DM.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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