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DNAL1 Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS205232

DNAL1 is an axonemal dynein light chain which functions as a component of the outer dynein arms complex. This complex acts as the molecular motor that provides the force to move cilia in an ATP-dependent manner. DNAL1 is expressed in tissues with motile cilia… More details

$230 / 0.02 mg +1 FREE 8GB USB
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
DNAL1, Recombinant Protein
Full Product Name
DNAL1, 1-190aa, Human, His tag, E Coli (Denatured)
Product Gene Name
Product Synonym Names
Dynein light chain 1 axonemal; Dynein light chain 1; axonemal; C14orf168; CILD16
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAKATTI KEALARWEEK TGQRPSEAKE IKLYAQIPPI EKMDASLSML ANCEKLSLST NCIEKIANLN GLKNLRILSL GRNNIKNLNG LEAVGDTLEE LWISYNFIEK LKGIHIMKKL KILYMSNNLV KDWAEFVKLA ELPCLEDLVF VGNPLEEKHS AENNWIEEAT KRVPKLKKLD GTPVIKGDEE EDN
Host
E Coli
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.4M urea, 10% glycerol
Purity/Purification
> 90% by SDS-PAGE
Concentration
1 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
23.9 kDa (213aa)
OMIM
AF542071 mRNA
3D Structure
ModBase 3D Structure for Q4LDG9
Antigen Species
Human
Tag Information
His-tag

Product Categories/Family for DNAL1 recombinant protein

Applications Tested/Suitable for DNAL1 recombinant protein
SDS-PAGE
Application Notes for DNAL1 recombinant protein
This protein is denatured. Denatured protein is most likely not the best option for functional studies. Denatured protein is better suited for Western Blot (WB) or imaging assays. Applications are user determined.

Product References and Citations for DNAL1 recombinant protein

Horvath J, Fliegauf M, et al. (2005). Am J Respir Cell Mol Biol. 33 (1):41-7.; Lancaster MA, et al. (2009). Curr Opin Genet Dev. 19 (3):220-9.

Other Notes

Small volumes of DNAL1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DNAL1 purchase

MBS205232 is a recombinant protein targeting DNAL1. Also known as DNAL1, 1-190aa, Human, His tag, E Coli (Denatured). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for DNAL1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
dynein light chain 1, axonemal isoform 1
NCBI Official Synonym Full Names
dynein axonemal light chain 1
NCBI Official Symbol
DNAL1
NCBI Official Synonym Symbols
LC1; CILD16; C14orf168
UniProt Protein Name
Dynein light chain 1, axonemal
Protein Family
UniProt Gene Name
DNAL1
UniProt Synonym Gene Names
LC1
NCBI Protein Information
dynein light chain 1, axonemal

NCBI Summary for DNAL1

This gene encodes an axonemal dynein light chain which functions as a component of the outer dynein arms complex. This complex acts as the molecular motor that provides the force to move cilia in an ATP-dependent manner. The encoded protein is expressed in tissues with motile cilia or flagella and may be involved in the movement of sperm flagella. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Jan 2011]
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UniProt Comments for DNAL1

Part of the multisubunit axonemal ATPase complexes that generate the force for cilia motility and govern beat frequency (). Component of the outer arm dynein (ODA). May be involved in a mechanosensory feedback mechanism controlling ODA activity based on external conformational cues by tethering the outer arm dynein heavy chain (DNAH5) to the microtubule within the axoneme (). Important for ciliary function in the airways and for the function of the cilia that produce the nodal flow essential for the determination of the left-right asymmetry (PubMed:21496787).
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with DNAL1 recombinant protein

 Reference Product  PubMed Publications
 DNAH5 recombinant protein  >2 publications with DNAL1 and DNAH5

Pathways associated with DNAL1 recombinant protein

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