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GTF2A1 Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS205261

TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity. Recombinant human GTF2A1 protein, fused to His-tag at… More details

$245 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GTF2A1, Recombinant Protein
Full Product Name
GTF2A1, 1-274aa, Human, His tag, E Coli
Product Synonym Names
Transcription initiation factor IIA subunit 1 isoform 1; General transcription factor IIA; 1; 19/37kDa; TF2A1; TFIIA; TFIIA-42; TFIIAL
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMANSANT NTVPKLYRSV IEDVINDVRD IFLDDGVDEQ VLMELKTLWE NKLMQSRAVD GFHSEEQQLL LQVQQQHQPQ QQQHHHHHHH QQAQPQQTVP QQAQTQQVLI PASQQATAPQ VIVPDSKLIQ HMNASNMSAA ATAATLALPA GVTPVQQILT NSGQLLQVVR AANGAQYIFQ PQQSVVLQQQ VIPQMQPGGV QAPVIQQVLA PLPGGISPQT GVIIQPQQIL FTGNKTQVIP TTVAAPTPAQ AQITATGQQQ PQAQPAQTQA PLVLQVD
Host
E Coli
Form/Format
Liquid. In 20mM Tris-HCl (pH 8.0) containing 20% glycerol, 0.15M NaCl, 1mM DTT
Purity/Purification
> 90% by SDS-PAGE
Concentration
0.5 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
32.2kDa (297aa) confirmed by MALDI-TOF
OMIM
X75383 mRNA
3D Structure
ModBase 3D Structure for P52655
Antigen Species
Human
Tag Information
His-tag
Applications Tested/Suitable for GTF2A1 recombinant protein
SDS-PAGE

Product References and Citations for GTF2A1 recombinant protein

Zhou H., Spicuglia S et al. (2006), Mol. Cell. Biol. 26:2728-2735

Other Notes

Small volumes of GTF2A1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GTF2A1 purchase

MBS205261 is a recombinant protein targeting GTF2A1. Also known as GTF2A1, 1-274aa, Human, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for GTF2A1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
transcription initiation factor IIA subunit 1 isoform 1
NCBI Official Synonym Full Names
general transcription factor IIA subunit 1
NCBI Official Symbol
GTF2A1
NCBI Official Synonym Symbols
TF2A1; TFIIA; TFIIAL; TFIIA-42
UniProt Protein Name
Transcription initiation factor IIA subunit 1
UniProt Synonym Protein Names
General transcription factor IIA subunit 1; TFIIAL; Transcription initiation factor TFIIA 42 kDa subunit; TFIIA-42
UniProt Gene Name
GTF2A1
UniProt Synonym Gene Names
TF2A1; TFIIA-42
NCBI Protein Information
transcription initiation factor IIA subunit 1

NCBI Summary for GTF2A1

Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and several general initiation factors (summarized by DeJong and Roeder, 1993 [PubMed 8224848]). One of these factors is TFIIA, which when purified from HeLa extracts consists of 35-, 19-, and 12-kD subunits.[supplied by OMIM, Jul 2010]
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UniProt Comments for GTF2A1

TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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