• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

POLR2H Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS205303

POLR2H is one of the essential subunits of RNA polymerase II that is shared by the other two eukaryotic DNA-directed RNA polymerases, I and III. Recombinant human POLR2H protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using… More details

$230 / 0.02 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
POLR2H, Recombinant Protein
Full Product Name
POLR2H, 1-150aa, Human His tag, E Coli
Product Synonym Names
DNA-directed RNA polymerases I II and III subunit RPABC3; DNA-directed RNA polymerases I; II; and III subunit RPABC3; hsRPB8; RPABC3; RPB17; RPB8
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAGILFE DIFDVKDIDP EGKKFDRVSR LHCESESFKM DLILDVNIQI YPVDLGDKFR LVIASTLYED GTLDDGEYNP TDDRPSRADQ FEYVMYGKVY RIEGDETSTE AATRLSAYVS YGGLLMRLQG DANNLHGFEV DSRVYLLMKK LAF
Host
E Coli
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
Purity/Purification
> 90% by SDS-PAGE
Concentration
0.5 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
19.5 kDa (173aa) confirmed by MALDI-TOF
OMIM
U37689 mRNA
3D Structure
ModBase 3D Structure for P52434
Antigen Species
Human
Tag Information
His-tag

Product Categories/Family for POLR2H recombinant protein

Applications Tested/Suitable for POLR2H recombinant protein
SDS-PAGE

Product References and Citations for POLR2H recombinant protein

Kang,X., et al. (2006) J. Biol. Chem. 281 (26), 18216-18226

Other Notes

Small volumes of POLR2H recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for POLR2H purchase

MBS205303 is a recombinant protein targeting POLR2H. Also known as POLR2H, 1-150aa, Human His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for POLR2H. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
DNA-directed RNA polymerases I, II, and III subunit RPABC3 isoform 2
NCBI Official Synonym Full Names
RNA polymerase II subunit H
NCBI Official Symbol
POLR2H
NCBI Official Synonym Symbols
RPB8; RPB17; RPABC3
UniProt Protein Name
DNA-directed RNA polymerases I, II, and III subunit RPABC3
UniProt Synonym Protein Names
DNA-directed RNA polymerase II subunit H; DNA-directed RNA polymerases I, II, and III 17.1 kDa polypeptide; RPB17; RPB8 homolog; hRPB8
UniProt Gene Name
POLR2H
UniProt Synonym Gene Names
RNA polymerases I, II, and III subunit ABC3; hRPB8
NCBI Protein Information
DNA-directed RNA polymerases I, II, and III subunit RPABC3

NCBI Summary for POLR2H

The three eukaryotic RNA polymerases are complex multisubunit enzymes that play a central role in the transcription of nuclear genes. This gene encodes an essential and highly conserved subunit of RNA polymerase II that is shared by the other two eukaryotic DNA-directed RNA polymerases, I and III. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]
Show more

UniProt Comments for POLR2H

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively.
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000