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IER3 Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS205338

IER3 functions in the protection of cells from Fas- or tumor necrosis factor type alpha-induced apoptosis. Partially degraded and unspliced transcripts are found after virus infection in vitro, but these transcripts are not found in vivo and do not generate a… More details

$250 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
IER3, Recombinant Protein
Full Product Name
IER3, 1-82aa, Human, His tag, E Coli
Product Gene Name
Product Synonym Names
Radiation-inducible immediate-early gene IEX-1; immediate early response 3; DIF-2; DIF2; GLY96; IEX-1; IEX-1L; IEX1; PRG1
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMCHSRSC HPTMTILQAP TPAPSTIPGP RRGSGPEIFT FDPLPEPAAA PAGRPSASRG HRKRSRRVLY PRVVRRQLPV EEPNP
Host
E Coli
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 2mM DTT
Purity/Purification
> 85% by SDS-PAGE
Concentration
0.25 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
11.4kDa (105aa), confirmed by MALDI-TOF
OMIM
S81914 mRNA
3D Structure
ModBase 3D Structure for P46695
Antigen Species
Human
Tag Information
His-tag

Product Categories/Family for IER3 recombinant protein

Applications Tested/Suitable for IER3 recombinant protein
SDS-PAGE

Product References and Citations for IER3 recombinant protein

Kondratyev AD, Chung KN, Jung MO (May 1996). Cancer Res 56 (7): 1498-502; Wu MX, Ao Z, Prasad KV, Wu R, Schlossman SF (Aug 1998). Science 281 (5379): 998-1001

Other Notes

Small volumes of IER3 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IER3 purchase

MBS205338 is a recombinant protein targeting IER3. Also known as IER3, 1-82aa, Human, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for IER3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
radiation-inducible immediate-early gene IEX-1
NCBI Official Synonym Full Names
immediate early response 3
NCBI Official Symbol
IER3
NCBI Official Synonym Symbols
DIF2; IEX1; PRG1; DIF-2; GLY96; IEX-1; IEX-1L
UniProt Protein Name
Radiation-inducible immediate-early gene IEX-1
UniProt Synonym Protein Names
Differentiation-dependent gene 2 protein; Protein DIF-2; Immediate early protein GLY96; Immediate early response 3 protein; PACAP-responsive gene 1 protein; Protein PRG1
UniProt Gene Name
IER3
UniProt Synonym Gene Names
DIF2; IEX1; PRG1; Protein DIF-2; Protein PRG1
NCBI Protein Information
radiation-inducible immediate-early gene IEX-1

NCBI Summary for IER3

This gene functions in the protection of cells from Fas- or tumor necrosis factor type alpha-induced apoptosis. Partially degraded and unspliced transcripts are found after virus infection in vitro, but these transcripts are not found in vivo and do not generate a valid protein. [provided by RefSeq, Jul 2008]
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UniProt Comments for IER3

May play a role in the ERK signaling pathway by inhibiting the dephosphorylation of ERK by phosphatase PP2A-PPP2R5C holoenzyme. Acts also as an ERK downstream effector mediating survival. As a member of the NUPR1/RELB/IER3 survival pathway, may provide pancreatic ductal adenocarcinoma with remarkable resistance to cell stress, such as starvation or gemcitabine treatment.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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