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POLR2I Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS205478

POLR2I, also known as RPB9, belongs to the archaeal rpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family. This protein is a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. DNA-dependent RNA polymerase… More details

$245 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
POLR2I, Recombinant Protein
Full Product Name
POLR2I, 1-125aa, Human, His tag, E Coli
Product Synonym Names
DNA-directed RNA polymerase II subunit RPB9; hRPB14.5; RPB9
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMEPDGTY EPGFVGIRFC QECNNMLYPK EDKENRILLY ACRNCDYQQE ADNSCIYVNK ITHEVDELTQ IIADVSQDPT LPRTEDHPCQ KCGHKEAVFF QSHSARAEDA MRLYYVCTAP HCGHRWTE
Host
E Coli
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.2M NaCl, 20% glycerol, 1mM DTT
Purity/Purification
> 85% by SDS-PAGE
Concentration
1 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
17.0kDa (148aa) confirmed by MALDI-TOF
OMIM
Z23102 Genomic DNA
3D Structure
ModBase 3D Structure for P36954
Antigen Species
Human
Tag Information
His-tag

Product Categories/Family for POLR2I recombinant protein

Applications Tested/Suitable for POLR2I recombinant protein
SDS-PAGE

Product References and Citations for POLR2I recombinant protein

Kershnar E., et al. (1998) J. Biol. Chem. 273:34444-34453

Other Notes

Small volumes of POLR2I recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for POLR2I purchase

MBS205478 is a recombinant protein targeting POLR2I. Also known as POLR2I, 1-125aa, Human, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for POLR2I. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
DNA-directed RNA polymerase II subunit RPB9
NCBI Official Synonym Full Names
RNA polymerase II subunit I
NCBI Official Symbol
POLR2I
NCBI Official Synonym Symbols
RPB9; hRPB14.5
UniProt Protein Name
DNA-directed RNA polymerase II subunit RPB9
UniProt Synonym Protein Names
DNA-directed RNA polymerase II subunit I; RNA polymerase II 14.5 kDa subunit; RPB14.5
UniProt Gene Name
POLR2I
UniProt Synonym Gene Names
RNA polymerase II subunit B9; RPB14.5
NCBI Protein Information
DNA-directed RNA polymerase II subunit RPB9

NCBI Summary for POLR2I

This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with two other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. The product of this gene has two zinc finger motifs with conserved cysteines and the subunit does possess zinc binding activity. [provided by RefSeq, Jul 2008]
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UniProt Comments for POLR2I

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB9 is part of the upper jaw surrounding the central large cleft and thought to grab the incoming DNA template ().
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with POLR2I recombinant protein

Organs/Tissues associated with POLR2I recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >1 publications with POLR2I and Vascular
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