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DEDD Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS205493

Death effector domain-containing protein, also known as DEDD, is a cytoplasmic protein. It translocates to the nucleus during CD95-mediated apoptosis, where it localizes to nucleoli-like structures, activates caspase-6 and specifically inhibits RNA polymerase… More details

$245 / 0.01 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
DEDD, Recombinant Protein
Full Product Name
DEDD, 1-318aa, Human, His tag, E Coli (Denatured)
Product Gene Name
Product Synonym Names
Death effector domain-containing protein; CASP8IP1; DEDD1; DEFT; FLDED1; KE05
Sequence
MGSSHHHHHH SSGLVPRGSH MAGLKRRASQ VWPEEHGEQE HGLYSLHRMF DIVGTHLTHR DVRVLSFLFV DVIDDHERGL IRNGRDFLLA LERQGRCDES NFRQVLQLLR IITRHDLLPY VTLKRRRAVC PDLVDKYLEE TSIRYVTPRA LSDPEPRPPQ PSKTVPPHYP VVCCPTSGPQ MCSKRPARGR ATLGSQRKRR KSVTPDPKEK QTCDIRLRVR AEYCQHETAL QGNVFSNKQD PLERQFERFN QANTILKSRD LGSIICDIKF SELTYLDAFW RDYINGSLLE ALKGVFITDS LKQAVGHEAI KLLVNVDEED YELGRQKLLR NLMLQALP
Host
E Coli
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.4M urea, 10% glycerol
Purity/Purification
> 85% by SDS-PAGE
Concentration
0.5 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
38.9 kDa (338aa)
OMIM
AF083236 mRNA
3D Structure
ModBase 3D Structure for O75618
Antigen Species
Human
Tag Information
His-tag

Product Categories/Family for DEDD recombinant protein

Applications Tested/Suitable for DEDD recombinant protein
SDS-PAGE
Application Notes for DEDD recombinant protein
This protein is denatured. Denatured protein is most likely not the best option for functional studies. Denatured protein is better suited for Western Blot (WB) or imaging assays. Applications are user determined.

Product References and Citations for DEDD recombinant protein

Stegh A H., et al. (1998) EMBO J. 17:5974-5986.; Schickling O., et al. (2001) Cell Death Differ. 8:1157-1168.

Other Notes

Small volumes of DEDD recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DEDD purchase

MBS205493 is a recombinant protein targeting DEDD. Also known as DEDD, 1-318aa, Human, His tag, E Coli (Denatured). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for DEDD. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
death effector domain-containing protein isoform b
NCBI Official Synonym Full Names
death effector domain containing
NCBI Official Symbol
DEDD
NCBI Official Synonym Symbols
DEFT; KE05; DEDD1; FLDED1; CASP8IP1
UniProt Protein Name
Death effector domain-containing protein
UniProt Synonym Protein Names
DEDPro1; Death effector domain-containing testicular molecule; FLDED-1
Protein Family
UniProt Gene Name
DEDD
UniProt Synonym Gene Names
DEDPRO1; DEFT
NCBI Protein Information
death effector domain-containing protein

NCBI Summary for DEDD

This gene encodes a protein that contains a death effector domain (DED). DED is a protein-protein interaction domain shared by adaptors, regulators and executors of the programmed cell death pathway. Overexpression of this gene was shown to induce weak apoptosis. Upon stimulation, this protein was found to translocate from cytoplasm to nucleus and colocalize with UBTF, a basal factor required for RNA polymerase I transcription, in the nucleolus. At least three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for DEDD

A scaffold protein that directs CASP3 to certain substrates and facilitates their ordered degradation during apoptosis. May also play a role in mediating CASP3 cleavage of KRT18. Regulates degradation of intermediate filaments during apoptosis. May play a role in the general transcription machinery in the nucleus and might be an important regulator of the activity of GTF3C3. Inhibits DNA transcription in vitro ().
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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