NELFE Recombinant Protein

Name: NELFE Recombinant Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS205569
Price: 230 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS205569

NELFE is a part of complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins; however, it has not been demonstrated that this protein binds RNA. It… More details

$230 / 0.004 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

NELFE, Recombinant Protein

Full Product Name

NELFE, 1-380aa, Human, His tag, E Coli

Product Gene Name

NELFE recombinant protein

Product Synonym Names

Negative elongation factor E; Negative elongation factor complex member E; D6S45; NELF-E; RD; RDBP; RDP

Sequence

MGSSHHHHHH SSGLVPRGSH MGSMLVIPPG LSEEEEALQK KFNKLKKKKK ALLALKKQSS SSTTSQGGVK RSLSEQPVMD TATATEQAKQ LVKSGAISAI KAETKNSGFK RSRTLEGKLK DPEKGPVPTF QPFQRSISAD DDLQESSRRP QRKSLYESFV SSSDRLRELG PDGEEAEGPG AGDGPPRSFD WGYEERSGAH SSASPPRSRS RDRSHERNRD RDRDRERDRD RDRDRDRERD RDRDRDRDRD RERDRDRERD RDRDREGPFR RSDSFPERRA PRKGNTLYVY GEDMTPTLLR GAFSPFGNII DLSMDPPRNC AFVTYEKMES ADQAVAELNG TQVESVQLKV NIARKQPMLD AATGKSVWGS LAVQNSPKGC HRDKRTQIVY SDDVYKENLV DGF

Host

E Coli

Form/Format

Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 5mM DTT

Purity/Purification

> 90% by SDS-PAGE

Concentration

0.25 mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

45.6kDa (403aa) confirmed by MALDI-TOF

3D Structure

ModBase 3D Structure for P18615

Antigen Species

Human

Tag Information

His-tag

Product Categories/Family for NELFE recombinant protein

Epigenomics (Transcription & Translation)

Applications Tested/Suitable for NELFE recombinant protein

SDS-PAGE

Product References and Citations for NELFE recombinant protein

Yamaguchi Y., Takagi T, et al. (1999) Cell 97:41-51; Surowy C.S., Hoganson G, et al. (1990) Gene 90:299-302;

Other Notes

Small volumes of NELFE recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NELFE purchase

MBS205569 is a recombinant protein targeting NELFE. Also known as NELFE, 1-380aa, Human, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for NELFE. It may not necessarily be applicable to this product.

NCBI GI #

14670268

NCBI GeneID

7936

NCBI Accession #

NP_002895

UniProt Primary Accession #

P18615

UniProt Secondary Accession #

Q5JP74; Q5JP75; Q96F56; Q9NPK2; A2BE08; B4DUN1; B4DYX9

UniProt Related Accession #

P18615

GenBank Nucleotide #

NM_002904.5

NCBI Official Full Name

negative elongation factor E

NCBI Official Synonym Full Names

negative elongation factor complex member E

NCBI Official Symbol

NELFE

NCBI Official Synonym Symbols

RD; RDP; RDBP; D6S45; NELF-E

UniProt Protein Name

Negative elongation factor E

UniProt Synonym Protein Names

RNA-binding protein RD

UniProt Gene Name

NELFE

UniProt Synonym Gene Names

RD; RDBP; NELF-E

NCBI Protein Information

negative elongation factor E

NCBI Summary for NELFE

The protein encoded by this gene is part of a complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins; however, it has not been demonstrated that this protein binds RNA. The protein contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D). The gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. [provided by RefSeq, Jul 2008]

UniProt Comments for NELFE

Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA. Binds to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1.

Research Articles on NELFE

1. A positively charged face of NELF-AC is involved in RNA binding, whereas the opposite face of the NELF-AC subcomplex binds NELF-B. NELF-B is predicted to form a HEAT repeat fold, also binds RNA in vivo, and anchors the subunit NELF-E, which is confirmed to bind RNA in vivo.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with NELFE recombinant protein

Reference Product	PubMed Publications
RDBP recombinant protein	>1 publications with NELFE and RDBP

Pathways associated with NELFE recombinant protein

Products by Pathway	Pathway Diagram
Abortive Elongation Of HIV-1 Transcript In The Absence Of Tat Pathway antibodies	Abortive Elongation Of HIV-1 Transcript In The Absence Of Tat Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
Formation Of HIV Elongation Complex In The Absence Of HIV Tat Pathway antibodies	Formation Of HIV Elongation Complex In The Absence Of HIV Tat Pathway Diagram
Formation Of HIV-1 Elongation Complex Containing HIV-1 Tat Pathway antibodies	Formation Of HIV-1 Elongation Complex Containing HIV-1 Tat Pathway Diagram
Formation Of RNA Pol II Elongation Complex Pathway antibodies	Formation Of RNA Pol II Elongation Complex Pathway Diagram
Formation Of The Early Elongation Complex Pathway antibodies	Formation Of The Early Elongation Complex Pathway Diagram
Formation Of The HIV-1 Early Elongation Complex Pathway antibodies	Formation Of The HIV-1 Early Elongation Complex Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
Generic Transcription Pathway antibodies	Generic Transcription Pathway Diagram
HIV Infection Pathway antibodies	HIV Infection Pathway Diagram

Diseases associated with NELFE recombinant protein

Disease Name	Pubmed Publications
Occupational Diseases Antibodies	>1 publications with NELFE and Occupational Diseases
Insulin Resistance Antibodies	>1 publications with NELFE and Insulin Resistance
Carcinoma, Hepatocellular Antibodies	>1 publications with NELFE and Carcinoma, Hepatocellular
Liver Neoplasms Antibodies	>1 publications with NELFE and Liver Neoplasms
Inflammation Antibodies	>1 publications with NELFE and Inflammation
Liver Diseases Antibodies	>1 publications with NELFE and Liver Diseases

Organs/Tissues associated with NELFE recombinant protein

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>3 publications with NELFE and Lung
Heart Antibodies	>1 publications with NELFE and Heart
Liver Antibodies	>1 publications with NELFE and Liver
Blood Antibodies	>1 publications with NELFE and Blood