SKA1 Recombinant Protein

Name: SKA1 Recombinant Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS205650
Price: 365 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS205650

SKA1, as known as spindle and kinetochore-associated protein 1, is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres… More details

$365 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

SKA1, Recombinant Protein

Full Product Name

SKA1, 1-255aa, Human, His tag, E Coli

Product Gene Name

SKA1 recombinant protein

Product Synonym Names

Spindle and kinetochore-associated protein 1; Spindle and kinetochore-associated protein 1; C18orf24

Sequence

MGSSHHHHHH SSGLVPRGSH MGSMASSDLE QLCSHVNEKI GNIKKTLSLR NCGQEPTLKT VLNKIGDEII VINELLNKLE LEIQYQEQTN NSLKELCESL EEDYKDIEHL KENVPSHLPQ VTVTQSCVKG SDLDPEEPIK VEEPEPVKKP PKEQRSIKEM PFITCDEFNG VPSYMKSRLT YNQINDVIKE INKAVISKYK ILHQPKKSMN SVTRNLYHRF IDEETKDTKG RYFIVEADIK EFTTLKADKK FHVLLNILRH CRRLSEVRGG GLTRYVIT

Host

E Coli

Form/Format

Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol 0.1M NaCl

Purity/Purification

> 80% by SDS-PAGE

Concentration

0.25 mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

31.9 kDa (278aa) confirmed by MALDI-TOF

OMIM

AK303464 mRNA

3D Structure

ModBase 3D Structure for Q96BD8

Antigen Species

Human

Tag Information

His-tag

Product Categories/Family for SKA1 recombinant protein

Cell Cycle

Applications Tested/Suitable for SKA1 recombinant protein

SDS-PAGE

Product References and Citations for SKA1 recombinant protein

Ota T. et al. (2004) Nat Genet. 36:40-45; Hanisch A. et al. (2006) EMBO J. 25: 5504-5515.;

Other Notes

Small volumes of SKA1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SKA1 purchase

MBS205650 is a recombinant protein targeting SKA1. Also known as SKA1, 1-255aa, Human, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for SKA1. It may not necessarily be applicable to this product.

NCBI GI #

88014625

NCBI GeneID

220134

NCBI Accession #

NP_001034624

UniProt Primary Accession #

Q96BD8

UniProt Secondary Accession #

B2R9Y6; B4E0P4

UniProt Related Accession #

Q96BD8

GenBank Nucleotide #

NM_001039535.2

NCBI Official Full Name

spindle and kinetochore-associated protein 1

NCBI Official Synonym Full Names

spindle and kinetochore associated complex subunit 1

NCBI Official Symbol

SKA1

NCBI Official Synonym Symbols

C18orf24

UniProt Protein Name

Spindle and kinetochore-associated protein 1

Protein Family

Spindle and kinetochore-associated protein

UniProt Gene Name

SKA1

UniProt Synonym Gene Names

C18orf24

NCBI Protein Information

spindle and kinetochore-associated protein 1

UniProt Comments for SKA1

Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation (PubMed:17093495, PubMed:19289083, PubMed:23085020). Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint (PubMed:17093495). The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies (PubMed:19289083). The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner (PubMed:19289083). Affinity for microtubules is synergistically enhanced in the presence of the ndc-80 complex and may allow the ndc-80 complex to track depolymerizing microtubules (PubMed:23085020). In the complex, it mediates the interaction with microtubules (PubMed:19289083, PubMed:23085020).

Research Articles on SKA1

1. Results show that SKA1 expression is regulated by mir-10a. Its knockdown inhibits migration and invasion in renal cell carcinoma cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with SKA1 recombinant protein

Reference Product	PubMed Publications
SKA3 recombinant protein	>15 publications with SKA1 and SKA3
SKA2 recombinant protein	>15 publications with SKA1 and SKA2
NDC80 recombinant protein	>8 publications with SKA1 and NDC80
AURKB recombinant protein	>4 publications with SKA1 and AURKB
CENPE recombinant protein	>1 publications with SKA1 and CENPE

Pathways associated with SKA1 recombinant protein

Products by Pathway	Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle, Mitotic Pathway antibodies	Cell Cycle, Mitotic Pathway Diagram
M Phase Pathway antibodies	M Phase Pathway Diagram
Mitotic Anaphase Pathway antibodies	Mitotic Anaphase Pathway Diagram
Mitotic Metaphase And Anaphase Pathway antibodies	Mitotic Metaphase And Anaphase Pathway Diagram
Mitotic Prometaphase Pathway antibodies	Mitotic Prometaphase Pathway Diagram
RHO GTPase Effectors Pathway antibodies	RHO GTPase Effectors Pathway Diagram
RHO GTPases Activate Formins Pathway antibodies	RHO GTPases Activate Formins Pathway Diagram
Resolution Of Sister Chromatid Cohesion Pathway antibodies	Resolution Of Sister Chromatid Cohesion Pathway Diagram
Separation Of Sister Chromatids Pathway antibodies	Separation Of Sister Chromatids Pathway Diagram

Diseases associated with SKA1 recombinant protein

Disease Name	Pubmed Publications
Kidney Diseases Antibodies	>5 publications with SKA1 and Kidney Diseases
Lung Neoplasms Antibodies	>3 publications with SKA1 and Lung Neoplasms
Breast Neoplasms Antibodies	>3 publications with SKA1 and Breast Neoplasms
Neoplasm Invasiveness Antibodies	>3 publications with SKA1 and Neoplasm Invasiveness
Neoplasm Metastasis Antibodies	>2 publications with SKA1 and Neoplasm Metastasis
Lung Diseases Antibodies	>2 publications with SKA1 and Lung Diseases
Liver Neoplasms Antibodies	>2 publications with SKA1 and Liver Neoplasms
Disease Models, Animal Antibodies	>2 publications with SKA1 and Disease Models, Animal
Kidney Neoplasms Antibodies	>1 publications with SKA1 and Kidney Neoplasms
Carcinoma, Hepatocellular Antibodies	>1 publications with SKA1 and Carcinoma, Hepatocellular

Organs/Tissues associated with SKA1 recombinant protein

Organ/Tissue Name	Pubmed Publications
Kidney Antibodies	>4 publications with SKA1 and Kidney
Lung Antibodies	>4 publications with SKA1 and Lung
Brain Antibodies	>3 publications with SKA1 and Brain
Liver Antibodies	>2 publications with SKA1 and Liver
Prostate Antibodies	>2 publications with SKA1 and Prostate
Blood Antibodies	>2 publications with SKA1 and Blood
Skin Antibodies	>1 publications with SKA1 and Skin
Eye Antibodies	>1 publications with SKA1 and Eye
Salivary Gland Antibodies	>1 publications with SKA1 and Salivary Gland