H2AFZ Recombinant Protein

Name: H2AFZ Recombinant Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS205659
Price: 245 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS205659

Histone H2A.Z, also known as H2AFZ, is a member of the histone H2A family. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped… More details

$245 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

H2AFZ, Recombinant Protein

Full Product Name

H2AFZ, 1-128aa, Human, His tag, E Coli (Denatured)

Product Gene Name

H2AFZ recombinant protein

Product Synonym Names

Histone H2A.Z; H2A.z; H2A/z; H2AZ

Sequence

MGSSHHHHHH SSGLVPRGSH MGSMAGGKAG KDSGKAKTKA VSRSQRAGLQ FPVGRIHRHL KSRTTSHGRV GATAAVYSAA ILEYLTAEVL ELAGNASKDL KVKRITPRHL QLAIRGDEEL DSLIKATIAG GGVIPHIHKS LIGKKGQQKT V

Host

E Coli

Form/Format

Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Purity/Purification

> 85% by SDS-PAGE

Concentration

0.25 mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

15.9 kDa (151aa)

OMIM

X52317 mRNA

3D Structure

ModBase 3D Structure for P0C0S5

Antigen Species

Human

Tag Information

His-tag

Product Categories/Family for H2AFZ recombinant protein

Epigenomics (Transcription & Translation)

Applications Tested/Suitable for H2AFZ recombinant protein

SDS-PAGE

Application Notes for H2AFZ recombinant protein

This protein is denatured. Denatured protein is most likely not the best option for functional studies. Denatured protein is better suited for Western Blot (WB) or imaging assays. Applications are user determined.

Product References and Citations for H2AFZ recombinant protein

Rupp R A., et al. (2005) Cell. 123: 1178-1179.; Martin C., et al. (2005) Mol Cell Biol. 6:838-849.;

Other Notes

Small volumes of H2AFZ recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for H2AFZ purchase

MBS205659 is a recombinant protein targeting H2AFZ. Also known as H2AFZ, 1-128aa, Human, His tag, E Coli (Denatured). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for H2AFZ. It may not necessarily be applicable to this product.

NCBI GI #

4504255

NCBI GeneID

3015

NCBI Accession #

NP_002097

UniProt Primary Accession #

P0C0S5

UniProt Secondary Accession #

P17317; Q6I9U0; B2RD56

UniProt Related Accession #

P0C0S5

GenBank Nucleotide #

NM_002106.3

NCBI Official Full Name

histone H2A.Z

NCBI Official Synonym Full Names

H2A histone family member Z

NCBI Official Symbol

H2AFZ

NCBI Official Synonym Symbols

H2AZ; H2A.z; H2A/z; H2A.Z-1

UniProt Protein Name

Histone H2A.Z

UniProt Gene Name

H2AFZ

UniProt Synonym Gene Names

H2AZ; H2A/z

NCBI Protein Information

histone H2A.Z

NCBI Summary for H2AFZ

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality. [provided by RefSeq, Jul 2008]

UniProt Comments for H2AFZ

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.

Research Articles on H2AFZ

1. Two possible modes of pioneering associated with combinations of H2A.Z and p300/CBP at nucleosome-occupied enhancers.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with H2AFZ recombinant protein

Products by Pathway	Pathway Diagram
Activated PKN1 Stimulates Transcription Of AR (androgen Receptor) Regulated Genes KLK2 And KLK3 Pathway antibodies	Activated PKN1 Stimulates Transcription Of AR (androgen Receptor) Regulated Genes KLK2 And KLK3 Pathway Diagram
Activation Of HOX Genes During Differentiation Pathway antibodies	Activation Of HOX Genes During Differentiation Pathway Diagram
Activation Of Anterior HOX Genes In Hindbrain Development During Early Embryogenesis Pathway antibodies	Activation Of Anterior HOX Genes In Hindbrain Development During Early Embryogenesis Pathway Diagram
Alcoholism Pathway antibodies	Alcoholism Pathway Diagram
Alcoholism Pathway antibodies	Alcoholism Pathway Diagram
Amyloid Fiber Formation Pathway antibodies	Amyloid Fiber Formation Pathway Diagram
B-WICH Complex Positively Regulates RRNA Expression Pathway antibodies	B-WICH Complex Positively Regulates RRNA Expression Pathway Diagram
C-MYB Transcription Factor Network Pathway antibodies	C-MYB Transcription Factor Network Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle, Mitotic Pathway antibodies	Cell Cycle, Mitotic Pathway Diagram

Diseases associated with H2AFZ recombinant protein

Disease Name	Pubmed Publications
Carcinoma Antibodies	>3 publications with H2AFZ and Carcinoma
Adenocarcinoma Antibodies	>3 publications with H2AFZ and Adenocarcinoma
Neoplasms, Experimental Antibodies	>2 publications with H2AFZ and Neoplasms, Experimental
Carcinoma, Hepatocellular Antibodies	>2 publications with H2AFZ and Carcinoma, Hepatocellular
Liver Diseases Antibodies	>2 publications with H2AFZ and Liver Diseases
Adenoma Antibodies	>2 publications with H2AFZ and Adenoma
Liver Neoplasms Antibodies	>2 publications with H2AFZ and Liver Neoplasms
Poisoning Antibodies	>1 publications with H2AFZ and Poisoning
Lung Neoplasms Antibodies	>1 publications with H2AFZ and Lung Neoplasms
Breast Neoplasms Antibodies	>1 publications with H2AFZ and Breast Neoplasms

Organs/Tissues associated with H2AFZ recombinant protein

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>4 publications with H2AFZ and Brain
Prostate Antibodies	>2 publications with H2AFZ and Prostate
Liver Antibodies	>2 publications with H2AFZ and Liver
Blood Antibodies	>2 publications with H2AFZ and Blood
Bladder Antibodies	>2 publications with H2AFZ and Bladder
Kidney Antibodies	>1 publications with H2AFZ and Kidney
Placenta Antibodies	>1 publications with H2AFZ and Placenta
Lung Antibodies	>1 publications with H2AFZ and Lung
Bone Marrow Antibodies	>1 publications with H2AFZ and Bone Marrow
Embryonic Tissue Antibodies	>1 publications with H2AFZ and Embryonic Tissue