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SIKE1 Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS205734

SIKE interacts with IKK-epsilon and TBK1 and acts as a suppressor of TLR3 and virus-triggered interferon activation pathways. Recombinant human SIKE1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional… More details

$230 / 0.004 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SIKE1, Recombinant Protein
Full Product Name
SIKE1, 1-207aa, Human, His tag, E Coli
Product Gene Name
Product Synonym Names
suppressor of IKBKE 1 isoform 2; suppressor of IKBKE 1 isoform 2; RP5-1000E10.4; SIKE
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMSCTIEK ILTDAKTLLE RLREHDAAAE SLVDQSAALH RRVAAMREAG TALPDQYQED ASDMKDMSKY KPHILLSQEN TQIRDLQQEN RELWISLEEH QDALELIMSK YRKQMLQLMV AKKAVDAEPV LKAHQSHSAE IESQIDRICE MGEVMRKAVQ VDDDQFCKIQ EKLAQLELEN KELRELLSIS SESLQARKEN SMDTASQAIK
Host
E Coli
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTT
Purity/Purification
> 85% by SDS-PAGE
Concentration
0.25 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
26.1 kDa (230aa) confirmed by MALDI-TOF
OMIM
AK024821 mRNA
3D Structure
ModBase 3D Structure for Q9BRV8
Antigen Species
Human
Tag Information
His-tag

Product Categories/Family for SIKE1 recombinant protein

Applications Tested/Suitable for SIKE1 recombinant protein
SDS-PAGE

Product References and Citations for SIKE1 recombinant protein

Huang J., et al (2005). EMBO J. 24:4018-4028; The MGC Project Team., et al (2004). Genome Res. 14:2121-2127;

Other Notes

Small volumes of SIKE1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SIKE1 purchase

MBS205734 is a recombinant protein targeting SIKE1. Also known as SIKE1, 1-207aa, Human, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for SIKE1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
suppressor of IKBKE 1 isoform 2
NCBI Official Synonym Full Names
suppressor of IKBKE 1
NCBI Official Symbol
SIKE1
NCBI Official Synonym Symbols
SIKE
UniProt Protein Name
Suppressor of IKBKE 1
UniProt Synonym Protein Names
Suppressor of IKK-epsilon
Protein Family
UniProt Gene Name
SIKE1
UniProt Synonym Gene Names
SIKE
NCBI Protein Information
suppressor of IKBKE 1

NCBI Summary for SIKE1

SIKE interacts with IKK-epsilon (IKBKE; MIM 605048) and TBK1 (MIM 604834) and acts as a suppressor of TLR3 (MIM 603029) and virus-triggered interferon activation pathways (Huang et al., 2005 [PubMed 16281057]).[supplied by OMIM, Mar 2008]

UniProt Comments for SIKE1

Physiological suppressor of IKK-epsilon and TBK1 that plays an inhibitory role in virus- and TLR3-triggered IRF3. Inhibits TLR3-mediated activation of interferon-stimulated response elements (ISRE) and the IFN-beta promoter. May act by disrupting the interactions of IKBKE or TBK1 with TICAM1/TRIF, IRF3 and DDX58/RIG-I. Does not inhibit NF-kappa-B activation pathways.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with SIKE1 recombinant protein

 Reference Product  PubMed Publications
 TANK recombinant protein  >1 publications with SIKE1 and TANK

Diseases associated with SIKE1 recombinant protein

Organs/Tissues associated with SIKE1 recombinant protein

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