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ATP5F1 Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS205757

ATP5F1 is a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked… More details

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Product Overview

Online Datasheet in PDF
Product Name
ATP5F1, Recombinant Protein
Full Product Name
ATP5F1, 83-256aa, Human, His tag, E Coli (Denatured)
Product Gene Name
ATP5F1 recombinant protein
Product Synonym Names
ATP synthase subunit b; PIG47
Sequence
MGSSHHHHHH SSGLVPRGSH MGSLILYALS KEIYVISAET FTALSVLGVM VYGIKKYGPF VADFADKLNE QKLAQLEEAK QASIQHIQNA IDTEKSQQAL VQKRHYLFDV QRNNIAMALE VTYRERLYRV YKEVKNRLDY HISVQNMMRR KEQEHMINWV EKHVVQSIST QQEKETIAKC IADLKLLAKK AQAQPVM
Host
E Coli
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.4M urea, 10% glycerol
Purity/Purification
> 80% by SDS-PAGE
Concentration
0.5 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
22.6 kDa (197aa)
OMIM
X60221 mRNA
3D Structure
ModBase 3D Structure for P24539
Antigen Species
Human
Tag Information
His-tag

Product Categories/Family for ATP5F1 recombinant protein

Metabolism
Applications Tested/Suitable for ATP5F1 recombinant protein
SDS-PAGE
Application Notes for ATP5F1 recombinant protein
This protein is denatured. Denatured protein is most likely not the best option for functional studies. Denatured protein is better suited for Western Blot (WB) or imaging assays. Applications are user determined.

SDS-Page of ATP5F1 recombinant protein

ATP5F1 Recombinant Protein – Cat# MBS205757

Product References and Citations for ATP5F1 recombinant protein

Higuti T., et al. (1991) Biochem. Biophys. Res. Commun. 178:1014-1020; Choudhary C., et al. (2009) Science. 325:834-840;

Other Notes

Small volumes of ATP5F1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ATP5F1 purchase

MBS205757 is a recombinant protein targeting ATP5F1. Also known as ATP5F1, 83-256aa, Human, His tag, E Coli (Denatured). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for ATP5F1. It may not necessarily be applicable to this product.

NCBI GI #
21361565
NCBI GeneID
515
NCBI Accession #
NP_001679
UniProt Primary Accession #
P24539
UniProt Secondary Accession #
Q9BQ68; Q9BRU8
UniProt Related Accession #
P24539
GenBank Nucleotide #
NM_001688.4
NCBI Official Full Name
ATP synthase F(0) complex subunit B1, mitochondrial
NCBI Official Synonym Full Names
ATP synthase peripheral stalk-membrane subunit b
NCBI Official Symbol
ATP5PB
NCBI Official Synonym Symbols
PIG47; ATP5F1
UniProt Protein Name
ATP synthase F(0) complex subunit B1, mitochondrial
UniProt Synonym Protein Names
ATP synthase proton-transporting mitochondrial F(0) complex subunit B1; ATP synthase subunit b; ATPase subunit b
Protein Family
ATP synthase F(0) complex
UniProt Gene Name
ATP5F1
UniProt Synonym Gene Names
ATPase subunit b
NCBI Protein Information
ATP synthase F(0) complex subunit B1, mitochondrial

NCBI Summary for ATP5F1

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the b subunit of the proton channel. [provided by RefSeq, Jul 2008]
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UniProt Comments for ATP5F1

Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F0 domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha3beta3 subcomplex and subunit a/ATP6 static relative to the rotary elements.
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Research Articles on ATP5F1

1. Down-regulation of mitochondrial ATPase by hypermethylation mechanism in chronic myeloid leukemia is associated with multidrug resistance.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
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