Baculovirus IL12 Active Protein – Cat# MBS205947

IL12 Baculovirus Active Protein

Name: IL12 Active Protein; Baculovirus; MyBioSource
Brand: MyBioSource
SKU: MBS205947
Price: 230 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS205947

IL12 is a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. It is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit and a 35 kD subunit. This cytokine is expressed by activated… More details

$230 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

IL12, Active Protein

Full Product Name

IL12, 23-328/23-219aa, Human, His tag, Baculovirus (Bioactivity Validated)

Product Gene Name

IL12 active protein

Product Synonym Names

Interleukin 12 (subunit beta/alpha); NKSF1

Sequence

* IL-12 is a heterodime r of IL12A and IL12B linked through a disulfide- bond between cysteines bolded in below sequences.
IL12A (p35)
R NLPVATPDP GMFPCLHHSQ NLLRAVSNML QKARQTLEFY PCTSEEIDHE DITKDKTSTV EACLPLELTK NESCLNSRET SFITNGSCLA SRKTSFMMAL CLSSIYEDLK MYQVEFKTMN AKLLMDPKRQ IFLDQNMLAV IDELMQALNF NSETVPQKSS LEEPDFYKTK IKLCILLHAF RIRAVTIDRV MSYLNASHHH HHH
IL1 2B (p40)
I WELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCS

Host

Baculovirus

Form/Format

Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol

Purity/Purification

> 90% by SDS-PAGE

Concentration

0.5 mg/ml (determined by Absorbance at 280nm) (lot specific)

Preparation and Storage

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

34.6kDa (p40) & 23.3kDa (p35) (306aa & 203aa) The heterodimeric IL12 has a predicted molecular weight of 57.9kDa (509aa). Molecular weight on SDS-PAGE will appear higher.

OMIM

M65291 mRNA

3D Structure

ModBase 3D Structure for P29459

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Antigen Species

Human

Tag Information

His-tag

Endotoxin Level

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Biological Activity

The activity is determined by the IFN-g ELISA in a using NK-92 cell. The ED50 for this effect is less than or equal to 0.03 ng/ml.

Product Categories/Family for IL12 active protein

Hormones & Cytokines

Applications Tested/Suitable for IL12 active protein

SDS-PAGE

Product References and Citations for IL12 active protein

Barton J.L., et al. (2000) Eur. J. Immunol. 30:3299-3308; Bazan J.F., et al. (2001) J. Immunol. 167:1440-1446

Other Notes

Small volumes of IL12 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IL12 purchase

MBS205947 is an active protein targeting IL12. Also known as IL12, 23-328/23-219aa, Human, His tag, Baculovirus (Bioactivity Validated). Host: Baculovirus.

Additional Details

NCBI/Uniprot data below describe general gene information for IL12. It may not necessarily be applicable to this product.

NCBI GI #

24430219

NCBI GeneID

3592

NCBI Accession #

NP_000873

NCBI Related Accession #

NP_002178

UniProt Primary Accession #

P29459

UniProt Secondary Accession #

Q96QZ1

UniProt Related Accession #

P29459

GenBank Nucleotide #

NM_000882.3

NCBI Official Full Name

interleukin-12 subunit alpha isoform 1

NCBI Official Synonym Full Names

interleukin 12A

NCBI Official Symbol

IL12A

NCBI Official Synonym Symbols

P35; CLMF; NFSK; NKSF1; IL-12A

UniProt Protein Name

Interleukin-12 subunit alpha

UniProt Synonym Protein Names

Cytotoxic lymphocyte maturation factor 35 kDa subunit; CLMF p35; IL-12 subunit p35; NK cell stimulatory factor chain 1; NKSF1

UniProt Gene Name

IL12A

UniProt Synonym Gene Names

NKSF1; IL-12A; CLMF p35; NKSF1

NCBI Protein Information

interleukin-12 subunit alpha

NCBI Summary for IL12

This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. [provided by RefSeq, Jul 2008]

UniProt Comments for IL12

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.

Research Articles on IL12

1. The anti-EGFR viroplexes with IL-12 and salmosin genes exhibited the most effective therapeutic efficacy in a mouse tumor model, especially when combined with doxorubicin.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with IL12 active protein

Reference Product	PubMed Publications
IL12B active protein	>77 publications with IL12 and IL12B
EBI3 active protein	>48 publications with IL12 and EBI3
IL1B active protein	>27 publications with IL12 and IL1B
IL4 active protein	>27 publications with IL12 and IL4
STAT4 active protein	>27 publications with IL12 and STAT4
IL12RB2 active protein	>24 publications with IL12 and IL12RB2
IFNG active protein	>23 publications with IL12 and IFNG
IL12RB1 active protein	>16 publications with IL12 and IL12RB1
STAT1 active protein	>13 publications with IL12 and STAT1
STAT3 active protein	>10 publications with IL12 and STAT3

Pathways associated with IL12 active protein

Products by Pathway	Pathway Diagram
African Trypanosomiasis Pathway antibodies	African Trypanosomiasis Pathway Diagram
African Trypanosomiasis Pathway antibodies	African Trypanosomiasis Pathway Diagram
Allograft Rejection Pathway antibodies	Allograft Rejection Pathway Diagram
Allograft Rejection Pathway antibodies	Allograft Rejection Pathway Diagram
Allograft Rejection Pathway antibodies	Allograft Rejection Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Aryl Hydrocarbon Receptor Pathway antibodies	Aryl Hydrocarbon Receptor Pathway Diagram
Chagas Disease (American Trypanosomiasis) Pathway antibodies	Chagas Disease (American Trypanosomiasis) Pathway Diagram
Chagas Disease (American Trypanosomiasis) Pathway antibodies	Chagas Disease (American Trypanosomiasis) Pathway Diagram

Diseases associated with IL12 active protein

Disease Name	Pubmed Publications
Inflammation Antibodies	>122 publications with IL12 and Inflammation
Necrosis Antibodies	>69 publications with IL12 and Necrosis
Disease Models, Animal Antibodies	>63 publications with IL12 and Disease Models, Animal
Liver Diseases Antibodies	>34 publications with IL12 and Liver Diseases
Multiple Sclerosis Antibodies	>25 publications with IL12 and Multiple Sclerosis
Liver Cirrhosis, Biliary Antibodies	>14 publications with IL12 and Liver Cirrhosis, Biliary
Neoplasms, Experimental Antibodies	>13 publications with IL12 and Neoplasms, Experimental
Lung Neoplasms Antibodies	>11 publications with IL12 and Lung Neoplasms
Heart Diseases Antibodies	>10 publications with IL12 and Heart Diseases
Liver Neoplasms Antibodies	>8 publications with IL12 and Liver Neoplasms

Organs/Tissues associated with IL12 active protein

Organ/Tissue Name	Pubmed Publications
Spleen Antibodies	>44 publications with IL12 and Spleen
Brain Antibodies	>24 publications with IL12 and Brain
Intestine Antibodies	>18 publications with IL12 and Intestine
Uterus Antibodies	>8 publications with IL12 and Uterus