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NT5C3B Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS205966

NT5C3B, also known 7-methylguanosine phosphate-specific 5'-nucleotidase, includes transferase activity and nucleotide binding. Among its related super-pathways are adenosine nucleotides degradation II and Pyrimidine metabolism. Specifically it hydrolyzes… More details

$230 / 0.02 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NT5C3B, Recombinant Protein
Full Product Name
NT5C3B, 1-300aa, Human, His tag, E Coli
Product Synonym Names
7-methylguanosine phosphate-specific 5'-nucleotidase; NT5C3L
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAEEVST LMKATVLMRQ PGRVQEIVGA LRKGGGDRLQ VISDFDMTLS RFAYNGKRCP SSYNILDNSK IISEECRKEL TALLHHYYPI EIDPHRTVKE KLPHMVEWWT KAHNLLCQQK IQKFQIAQVV RESNAMLREG YKTFFNTLYH NNIPLFIFSA GIGDILEEII RQMKVFHPNI HIVSNYMDFN EDGFLQGFKG QLIHTYNKNS SACENSGYFQ QLEGKTNVIL LGDSIGDLTM ADGVPGVQNI LKIGFLNDKV EERRERYMDS YDIVLEKDET LDVVNGLLQH ILCQGVQLEM QGP
Host
E Coli
Form/Format
Liquid. Phosphate buffered saline (pH 7.4) containing 20% glycerol, 1mM DTT
Purity/Purification
> 85% by SDS-PAGE
Concentration
1 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
36.8 kDa (323aa) confirmed by MALDI-TOF
3D Structure
ModBase 3D Structure for Q969T7
Antigen Species
Human
Tag Information
His-tag
Applications Tested/Suitable for NT5C3B recombinant protein
SDS-PAGE

Product References and Citations for NT5C3B recombinant protein

Brieger A. et al. (2010) Proteomics. 10:3343-3355.; Aksoy P. et al. (2009) Pharmacogenet Genomics. 19:567-576.;

Other Notes

Small volumes of NT5C3B recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NT5C3B purchase

MBS205966 is a recombinant protein targeting NT5C3B. Also known as NT5C3B, 1-300aa, Human, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for NT5C3B. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
7-methylguanosine phosphate-specific 5'-nucleotidase
NCBI Official Synonym Full Names
5'-nucleotidase, cytosolic IIIB
NCBI Official Symbol
NT5C3B
NCBI Official Synonym Symbols
NT5C3L; cN-IIIB
UniProt Protein Name
7-methylguanosine phosphate-specific 5'-nucleotidase
UniProt Synonym Protein Names
Cytosolic 5'-nucleotidase 3B; Cytosolic 5'-nucleotidase III-like protein
UniProt Gene Name
NT5C3B
UniProt Synonym Gene Names
NT5C3L; cN-III-like protein
NCBI Protein Information
7-methylguanosine phosphate-specific 5'-nucleotidase

UniProt Comments for NT5C3B

Specifically hydrolyzes 7-methylguanosine monophosphate (m7GMP) to 7-methylguanosine and inorganic phosphate (PubMed:23223233, PubMed:24603684). The specific activity for m7GMP may protect cells against undesired salvage of m7GMP and its incorporation into nucleic acids (PubMed:23223233). Also has weak activity for CMP (PubMed:23223233, PubMed:24603684). UMP and purine nucleotides are poor substrates (PubMed:23223233).
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with NT5C3B recombinant protein

Organs/Tissues associated with NT5C3B recombinant protein

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