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TNF Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS205974

Tnf also known as Tumor necrosis factor. The Protein acts as a cytokine binds TNF receptors plays a role in regulation of cell proliferation, induction of apoptosis, and inflammatory response. Recombinant rat Tnf, fused to His-tag at N-terminus, was expressed… More details

$230 / 0.02 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Tnf, Recombinant Protein
Full Product Name
Tnf, 80-235aa, Rat, His tag, E Coli
Product Gene Name
Product Synonym Names
Tumor necrosis factor; RATTNF; TNF-alpha; Tnfa
Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMLRSSS QNSSDKPVAH VVANHQAEEQ LEWLSQRANA LLANGMDLKD NQLVVPADGL YLIYSQVLFK GQGCPDYVLL THTVSRFAIS YQEKVSLLSA IKSPCPKDTP EGAELKPWYE PMYLGGVFQL EKGDLLSAEV NLPKYLDITE SGQVYFGVIA L
Host
E Coli
Form/Format
Liquid. In phosphate buffer saline (pH 7.4) containing 10% glycerol, 1mM DTT
Purity/Purification
> 90% by SDS-PAGE
Concentration
1mg/ml (determined by Absorbance at 280nm) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
19.9kDa (181aa), confirmed by MALDI-TOF
3D Structure
ModBase 3D Structure for P16599
Antigen Species
Rat
Tag Information
His-tag

Product Categories/Family for Tnf recombinant protein

Applications Tested/Suitable for Tnf recombinant protein
SDS-PAGE

Product References and Citations for Tnf recombinant protein

Furuya T., et al. (2001) Genes Immun. 2:229-232.; Warle M.C., et al. (2005) Transpl. Immunol. 14:77-82.

Other Notes

Small volumes of Tnf recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Tnf purchase

MBS205974 is a recombinant protein targeting Tnf. Also known as Tnf, 80-235aa, Rat, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for Tnf. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
tumor necrosis factor
NCBI Official Synonym Full Names
tumor necrosis factor
NCBI Official Symbol
Tnf
NCBI Official Synonym Symbols
Tnfa; RATTNF; TNF-alpha
UniProt Protein Name
Tumor necrosis factor
UniProt Synonym Protein Names
Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
Protein Family
UniProt Gene Name
Tnf
UniProt Synonym Gene Names
Tnfa; Tnfsf2; TNF-a; NTF; ICD1; ICD2
NCBI Protein Information
tumor necrosis factor

NCBI Summary for Tnf

acts as a cytokine; binds TNF receptors; plays a role in regulation of cell proliferation, induction of apoptosis, and inflammatory response [RGD, Feb 2006]

UniProt Comments for Tnf

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with Tnf recombinant protein

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