TRIB3 Recombinant Protein

Name: TRIB3 Recombinant Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS205996
Price: 230 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS205996

TRIB3, also known as tribbles pseudokinase 3, is a putative protein kinase that is induced by the transcription factor NF-kappaB. This protein is a negative regulator of NF-kappaB, and can also sensitize cells to TNF- and TRAIL-induced apoptosis. In addition,… More details

$230 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

TRIB3, Recombinant Protein

Full Product Name

TRIB3, 1-358aa, Human, His tag, E Coli (Denatured)

Product Gene Name

TRIB3 recombinant protein

Product Synonym Names

Tribbles homolog 3; Tribbles homolog 3; C20orf97; NIPK; SINK; SKIP3; TRB3

Sequence

MGSSHHHHHH SSGLVPRGSH MGSMRATPLA APAGSLSRKK RLELDDNLDT ERPVQKRARS GPQPRLPPCL LPLSPPTAPD RATAVATASR LGPYVLLEPE EGGRAYQALH CPTGTEYTCK VYPVQEALAV LEPYARLPPH KHVARPTEVL AGTQLLYAFF TRTHGDMHSL VRSRHRIPEP EAAVLFRQMA TALAHCHQHG LVLRDLKLCR FVFADRERKK LVLENLEDSC VLTGPDDSLW DKHACPAYVG PEILSSRASY SGKAADVWSL GVALFTMLAG HYPFQDSEPV LLFGKIRRGA YALPAGLSAP ARCLVRCLLR REPAERLTAT GILLHPWLRQ DPMPLAPTRS HLWEAAQVVP DGLGLDEARE EEGDREVVLY G

Host

E Coli

Form/Format

Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol

Purity/Purification

> 85% by SDS-PAGE

Concentration

0.25 mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

42.0 kDa (381aa)

OMIM

AF250311 mRNA

3D Structure

ModBase 3D Structure for Q96RU7

Antigen Species

Human

Tag Information

His-tag

Product Categories/Family for TRIB3 recombinant protein

Signal Transduction

Applications Tested/Suitable for TRIB3 recombinant protein

SDS-PAGE

Application Notes for TRIB3 recombinant protein

This protein is denatured. Denatured protein is most likely not the best option for functional studies. Denatured protein is better suited for Western Blot (WB) or imaging assays. Applications are user determined.

Product References and Citations for TRIB3 recombinant protein

Du K. et al. (2003) Science. 300:1574-1577.; Hegedus Z. et al. (2006) Cell Mol Life Sci. 63:1632-1641.;

Other Notes

Small volumes of TRIB3 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TRIB3 purchase

MBS205996 is a recombinant protein targeting TRIB3. Also known as TRIB3, 1-358aa, Human, His tag, E Coli (Denatured). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for TRIB3. It may not necessarily be applicable to this product.

NCBI GI #

31542265

NCBI GeneID

57761

NCBI Accession #

NP_066981

UniProt Primary Accession #

Q96RU7

UniProt Secondary Accession #

Q53GU4; Q53ZW7; Q6I9Y9; Q8TAI6; Q9H5M8; Q9NUD2

UniProt Related Accession #

Q96RU7

GenBank Nucleotide #

NM_021158.4

NCBI Official Full Name

tribbles homolog 3 isoform 1

NCBI Official Synonym Full Names

tribbles pseudokinase 3

NCBI Official Symbol

TRIB3

NCBI Official Synonym Symbols

NIPK; SINK; TRB3; SKIP3; C20orf97

UniProt Protein Name

Tribbles homolog 3

UniProt Synonym Protein Names

Neuronal cell death-inducible putative kinase; SINK; p65-interacting inhibitor of NF-kappa-B

Protein Family

Tribbles

UniProt Gene Name

TRIB3

UniProt Synonym Gene Names

C20orf97; NIPK; SKIP3; TRB3; TRB-3

NCBI Protein Information

tribbles homolog 3

NCBI Summary for TRIB3

The protein encoded by this gene is a putative protein kinase that is induced by the transcription factor NF-kappaB. The encoded protein is a negative regulator of NF-kappaB and can also sensitize cells to TNF- and TRAIL-induced apoptosis. In addition, this protein can negatively regulate the cell survival serine-threonine kinase AKT1. Differential promoter usage and alternate splicing result in multiple transcript variants. [provided by RefSeq, Jul 2014]

UniProt Comments for TRIB3

Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. Binds to ATF4 and inhibits its transcriptional activation activity. Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. Interacts with MAPK kinases and regulates activation of MAP kinases. May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. Does not display kinase activity. Inhibits the transcriptional activity of DDIT3/CHOP and is involved in DDIT3/CHOP-dependent cell death during ER stress. Can inhibit APOBEC3A editing of nuclear DNA.

Research Articles on TRIB3

1. TRIB3, a single-domain protein with impaired kinase catalytic activity, has been described to perform a complex of roles, many of which are likely to be cell type-specific. (Review)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with TRIB3 recombinant protein

Reference Product	PubMed Publications
ATF4 recombinant protein	>47 publications with TRIB3 and ATF4
AKT1 recombinant protein	>8 publications with TRIB3 and AKT1
AKT2 recombinant protein	>2 publications with TRIB3 and AKT2

Pathways associated with TRIB3 recombinant protein

Products by Pathway	Pathway Diagram
Activation Of AKT2 Pathway antibodies	Activation Of AKT2 Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
Adipogenesis Pathway antibodies	Adipogenesis Pathway Diagram
CD28 Co-stimulation Pathway antibodies	CD28 Co-stimulation Pathway Diagram
CD28 Dependent PI3K/Akt Signaling Pathway antibodies	CD28 Dependent PI3K/Akt Signaling Pathway Diagram
Costimulation By The CD28 Family Pathway antibodies	Costimulation By The CD28 Family Pathway Diagram
DAP12 Interactions Pathway antibodies	DAP12 Interactions Pathway Diagram
DAP12 Signaling Pathway antibodies	DAP12 Signaling Pathway Diagram
Downstream Signal Transduction Pathway antibodies	Downstream Signal Transduction Pathway Diagram
Downstream Signaling Events Of B Cell Receptor (BCR) Pathway antibodies	Downstream Signaling Events Of B Cell Receptor (BCR) Pathway Diagram

Diseases associated with TRIB3 recombinant protein

Disease Name	Pubmed Publications
Insulin Resistance Antibodies	>50 publications with TRIB3 and Insulin Resistance
Inflammation Antibodies	>22 publications with TRIB3 and Inflammation
Disease Models, Animal Antibodies	>22 publications with TRIB3 and Disease Models, Animal
Necrosis Antibodies	>12 publications with TRIB3 and Necrosis
Liver Diseases Antibodies	>12 publications with TRIB3 and Liver Diseases
Breast Neoplasms Antibodies	>11 publications with TRIB3 and Breast Neoplasms
Metabolic Syndrome Antibodies	>11 publications with TRIB3 and Metabolic Syndrome
Kidney Diseases Antibodies	>9 publications with TRIB3 and Kidney Diseases
Nervous System Diseases Antibodies	>9 publications with TRIB3 and Nervous System Diseases
Fibrosis Antibodies	>8 publications with TRIB3 and Fibrosis

Organs/Tissues associated with TRIB3 recombinant protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>45 publications with TRIB3 and Blood
Liver Antibodies	>34 publications with TRIB3 and Liver
Muscle Antibodies	>30 publications with TRIB3 and Muscle
Bone Antibodies	>17 publications with TRIB3 and Bone
Vascular Antibodies	>16 publications with TRIB3 and Vascular
Kidney Antibodies	>16 publications with TRIB3 and Kidney
Pancreas Antibodies	>12 publications with TRIB3 and Pancreas
Lung Antibodies	>12 publications with TRIB3 and Lung
Bone Marrow Antibodies	>10 publications with TRIB3 and Bone Marrow
Brain Antibodies	>10 publications with TRIB3 and Brain