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GAD1-iso1 Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS206007

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A… More details

$230 / 0.02 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GAD1-iso1, Recombinant Protein
Full Product Name
GAD1-iso1, 1-594aa, Human, His tag, E Coli (Denatured)
Product Synonym Names
Glutamate decarboxylase 1; CPSQ1; GAD; SCP
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMASSTPS SSATSSNAGA DPNTTNLRPT TYDTWCGVAH GCTRKLGLKI CGFLQRTNSL EEKSRLVSAF KERQSSKNLL SCENSDRDAR FRRTETDFSN LFARDLLPAK NGEEQTVQFL LEVVDILLNY VRKTFDRSTK VLDFHHPHQL LEGMEGFNLE LSDHPESLEQ ILVDCRDTLK YGVRTGHPRF FNQLSTGLDI IGLAGEWLTS TANTNMFTYE IAPVFVLMEQ ITLKKMREIV GWSSKDGDGI FSPGGAISNM YSIMAARYKY FPEVKTKGMA AVPKLVLFTS EQSHYSIKKA GAALGFGTDN VILIKCNERG KIIPADFEAK ILEAKQKGYV PFYVNATAGT TVYGAFDPIQ EIADICEKYN LWLHVDAAWG GGLLMSRKHR HKLNGIERAN SVTWNPHKMM GVLLQCSAIL VKEKGILQGC NQMCAGYLFQ PDKQYDVSYD TGDKAIQCGR HVDIFKFWLM WKAKGTVGFE NQINKCLELA EYLYAKIKNR EEFEMVFNGE PEHTNVCFWY IPQSLRGVPD SPQRREKLHK VAPKIKALMM ESGTTMVGYQ PQGDKANFFR MVISNPAATQ SDIDFLIEEI ERLGQDL
Host
E Coli
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol
Purity/Purification
> 80% by SDS-PAGE
Concentration
0.5 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
69.3 kDa (617aa)
OMIM
M81883 mRNA
3D Structure
ModBase 3D Structure for Q99259
Antigen Species
Human
Tag Information
His-tag

Product Categories/Family for GAD1-iso1 recombinant protein

Applications Tested/Suitable for GAD1-iso1 recombinant protein
SDS-PAGE
Application Notes for GAD1-iso1 recombinant protein
This protein is denatured. Denatured protein is most likely not the best option for functional studies. Denatured protein is better suited for Western Blot (WB) or imaging assays. Applications are user determined.

Product References and Citations for GAD1-iso1 recombinant protein

Lynex C.N., et al. (2004) BMC Neurol. 4:20-20

Other Notes

Small volumes of GAD1-iso1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GAD1-iso1 purchase

MBS206007 is a recombinant protein targeting GAD1-iso1. Also known as GAD1-iso1, 1-594aa, Human, His tag, E Coli (Denatured). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for GAD1-iso1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
glutamate decarboxylase 1 isoform GAD67
NCBI Official Synonym Full Names
glutamate decarboxylase 1
NCBI Official Symbol
GAD1
NCBI Official Synonym Symbols
GAD; SCP; CPSQ1
UniProt Protein Name
Glutamate decarboxylase 1
UniProt Synonym Protein Names
67 kDa glutamic acid decarboxylase; GAD-67; Glutamate decarboxylase 67 kDa isoform
UniProt Gene Name
GAD1
UniProt Synonym Gene Names
GAD; GAD67; GAD-67
NCBI Protein Information
glutamate decarboxylase 1

NCBI Summary for GAD1-iso1

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq, Jul 2008]
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UniProt Comments for GAD1-iso1

Catalyzes the production of GABA.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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