gldA Active Protein

Name: gldA Active Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS206011
Price: 180 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS206011

gldA catalyzes the NAD-dependent oxidation of glycerol to dihydroxyacetone (glycerone). This protein allows microorganisms to utilize glycerol as a source of carbon under anaerobic conditions. In E Coli, an important role of GldA is also likely to regulate… More details

$180 / 0.004 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

gldA, Active Protein

Full Product Name

gldA, 1-367aa, E Coli, His tag, E Coli (Bioactivity Validated)

Product Gene Name

gldA active protein

Product Synonym Names

Glycerol dehydrogenase; ECK3937; JW5556

Sequence

MGSSHHHHHH SSGLVPRGSH MGSMDRIIQS PGKYIQGADV INRLGEYLKP LAERWLVVGD KFVLGFAQST VEKSFKDAGL VVEIAPFGGE CSQNEIDRLR GIAETAQCGA ILGIGGGKTL DTAKALAHFM GVPVAIAPTI ASTDAPCSAL SVIYTDEGEF DRYLLLPNNP NMVIVDTKIV AGAPARLLAA GIGDALATWF EARACSRSGA TTMAGGKCTQ AALALAELCY NTLLEEGEKA MLAAEQHVVT PALERVIEAN TYLSGVGFES GGLAAAHAVH NGLTAIPDAH HYYHGEKVAF GTLTQLVLEN APVEEIETVA ALSHAVGLPI TLAQLDIKED VPAKMRIVAE AACAEGETIH NMPGGATPDQ VYAALLVADQ YGQRFLQEWE

Host

E Coli

Form/Format

Liquid. In phosphate buffered saline (pH 7.4), 10% glycerol

Purity/Purification

> 95% by SDS-PAGE

Concentration

1 mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

41.1 kDa (390aa), confirmed by MALDI-TOF

3D Structure

ModBase 3D Structure for P0A9S5

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Antigen Species

E Coli

Tag Information

His-tag

Biological Activity

Specific activity: > 14 Units/ml One unit will oxidize 1.0 umole of glycerol to dihydroxyacetone per minute at pH 8.0 at 25C

Assay

1. Prepare a 200ul reaction mix into a suitable container: The final concentrations are 93mM Glycine, 93mM Potassium chloride, 2375mM Glycerol, 3mM b-NAD.
2. Equilibrate to 25C and monitor at A340nm until the value is constant using a spectrophotometer.
3. Add 20ul of recombinant gldA protein with various concentrations (0.2ug, 0.1ug, 0.05ug) in 180ul reaction buffer.
4. Mix by inversion and record the decrease at A340nm for 10 minutes.

Product Categories/Family for gldA active protein

Signal Transduction

Applications Tested/Suitable for gldA active protein

SDS-PAGE

Product References and Citations for gldA active protein

Subedi K.P., et al. (2008) FEMS Microbiol. Lett. 279:180-187; Gonzalez R., et al. (2008) Metab. Eng. 10:234-245

Other Notes

Small volumes of gldA active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for gldA purchase

MBS206011 is an active protein targeting gldA. Also known as gldA, 1-367aa, E Coli, His tag, E Coli (Bioactivity Validated). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for gldA. It may not necessarily be applicable to this product.

NCBI GI #

90111668

NCBI GeneID

948440

NCBI Accession #

NP_418380

UniProt Primary Accession #

P0A9S5

UniProt Secondary Accession #

P32665; P78132; Q2M8P1

GenBank Nucleotide #

NC_000913.3

NCBI Official Full Name

glycerol dehydrogenase, NAD+ dependent; 1,2-propanediol:NAD+ oxidoreductase

NCBI Official Symbol

gldA

NCBI Official Synonym Symbols

ECK3937; JW5556

UniProt Protein Name

Glycerol dehydrogenase

UniProt Gene Name

gldA

UniProt Synonym Gene Names

GDH; GLDH

NCBI Protein Information

glycerol dehydrogenase, NAD+ dependent; 1,2-propanediol:NAD+ oxidoreductase

NCBI Summary for gldA

The enzyme is found in two catalytically active forms, a large form of eight subunits and a small form of two subunits. [More information is available at EcoCyc: EG11904].

UniProt Comments for gldA

Catalyzes the NAD-dependent oxidation of glycerol to dihydroxyacetone (glycerone). Allows microorganisms to utilize glycerol as a source of carbon under anaerobic conditions. In E.coli, an important role of GldA is also likely to regulate the intracellular level of dihydroxyacetone by catalyzing the reverse reaction, i.e. the conversion of dihydroxyacetone into glycerol. Possesses a broad substrate specificity, since it is also able to oxidize 1,2-propanediol and to reduce glycolaldehyde, methylglyoxal and hydroxyacetone into ethylene glycol, lactaldehyde and 1,2-propanediol, respectively.

Research Articles on gldA

1. this study proposes that the primary role of gldA is to remove toxic dihydroxyacetone by converting it into glycerol.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with gldA active protein

Reference Product	PubMed Publications
glpK active protein	>6 publications with gldA and glpK
dhaK active protein	>5 publications with gldA and dhaK
fucO active protein	>2 publications with gldA and fucO
ptsA active protein	>1 publications with gldA and ptsA
adhE active protein	>1 publications with gldA and adhE
dhaL active protein	>1 publications with gldA and dhaL

Pathways associated with gldA active protein

Products by Pathway	Pathway Diagram
Glycerolipid Metabolism Pathway antibodies	Glycerolipid Metabolism Pathway Diagram
Glycerolipid Metabolism Pathway antibodies	Glycerolipid Metabolism Pathway Diagram
Metabolic Pathways antibodies	Metabolic Pathways Diagram
Propanoate Metabolism Pathway antibodies	Propanoate Metabolism Pathway Diagram
Propanoate Metabolism Pathway antibodies	Propanoate Metabolism Pathway Diagram
Aminopropanol Phosphate Biosynthesis II Pathway antibodies	Aminopropanol Phosphate Biosynthesis II Pathway Diagram
Glycerol Degradation V Pathway antibodies	Glycerol Degradation V Pathway Diagram
Glycerol Degradation V Pathway antibodies	Glycerol Degradation V Pathway Diagram
Methylglyoxal Degradation III Pathway antibodies	Methylglyoxal Degradation III Pathway Diagram
Methylglyoxal Degradation III Pathway antibodies	Methylglyoxal Degradation III Pathway Diagram