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CST3 Baculovirus Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS206152

CST3, also known as cystain-C, is secreted type 2 cysteine proteinase inhibitors of the cystatin superfamily. It is involved in processes such as tumor invasion and metastasis, inflammation and some neurological diseases. It inhibits many cysteine proteases… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Cst3, Active Protein
Full Product Name
Cst3, 21-140aa, Rat, His tag, Baculovirus (Bioactivity Validated)
Product Gene Name
Product Synonym Names
Cystain-C; CST3; CYSC
Sequence
GTSRPPPRLL GAPQEADASE EGVQRALDFA VSEYNKGSND AYHSRAIQVV RARKQLVAGI NYYLDVEMGR TTCTKSQTNL TNCPFHDQPH LMRKALCSFQ IYSVPWKGTH TLTKSSCKNA LEHHHHHH
Host
Baculovirus
Form/Format
Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
Purity/Purification
> 95% by SDS-PAGE
Concentration
0.5 mg/ml (determined by Absorbance at 280nm) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
14.3kDa (128aa) 13.5-18KDa (SDS-PAGE under reducing conditions.)
3D Structure
ModBase 3D Structure for P14841
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Antigen Species
Rat
Tag Information
His-tag
Endotoxin Level
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Biological Activity
The IC50 value is < 1.0nM. The inhibitory function of Cystatin 3 on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C.

Product Categories/Family for Cst3 active protein

Applications Tested/Suitable for Cst3 active protein
SDS-PAGE

Product References and Citations for Cst3 active protein

Reed CH., et al. (2000) Br J Biomed. Sci. 57:323-329.; Esnard A., et al. (1992) FEBS Lett. 300:131-135.

Other Notes

Small volumes of Cst3 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Cst3 purchase

MBS206152 is an active protein targeting Cst3. Also known as Cst3, 21-140aa, Rat, His tag, Baculovirus (Bioactivity Validated). Host: Baculovirus.

Additional Details

NCBI/Uniprot data below describe general gene information for Cst3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
cystatin-C
NCBI Official Synonym Full Names
cystatin C
NCBI Official Symbol
Cst3
NCBI Official Synonym Symbols
CYSC
UniProt Protein Name
Cystatin-C
UniProt Synonym Protein Names
Cystatin-3
Protein Family
UniProt Gene Name
Cst3
NCBI Protein Information
cystatin-C

NCBI Summary for Cst3

extracellular cysteine protease inhibitor that may be involved in amyloidosis [RGD, Feb 2006]

UniProt Comments for Cst3

As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity. Known to inhibit cathepsin B, H, and L.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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