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N-Terminal Xaa-Pro-Lys N-Methyltransferase1 (NTMT1) Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS206159

NTMT1 also known as N-terminal Xaa-Pro-Lys N-methyltrasferase1 isoform a. NTMT1 is catalyzes the transfer of the methyl group from the S-adenosyl-l-methionine to the protein alpha-amine, resulting in formation of S-adenosyl-l-homocysteine and… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
N-terminal Xaa-Pro-Lys N-methyltransferase1 (NTMT1), Recombinant Protein
Full Product Name
N-terminal Xaa-Pro-Lys N-methyltransferase1 isoform a, 1-223aa, Human, His tag, E Coli
Product Gene Name
NTMT1 recombinant protein
Product Synonym Names
AD-003; C9orf32; HOMT1A; METTL11A; NRMT; NTM1A; NTMT1
Sequence
MGSSHHHHHH SSGLVPRGSH MGSEFMTSEV IEDEKQFYSK AKTYWKQIPP TVDGMLGGYG HISSIDINSS RKFLQRFLRE GPNKTGTSCA LDCGAGIGRI TKRLLLPLFR EVDMVDITED FLVQAKTYLG EEGKRVRNYF CCGLQDFTPE PDSYDVIWIQ WVIGHLTDQH LAEFLRRCKG SLRPNGIIVI KDNMAQEGVI LDDVDSSVCR DLDVVRRIIC SAGLSLLAEE RQENLPDEIY HVYSFALR
Host
E Coli
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol
Purity/Purification
> 95% by SDS-PAGE
Concentration
0.5 mg/ml (determined by Absorbance at 280nm) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
28.1kDa (248aa)
OMIM
AF110776 mRNA
3D Structure
ModBase 3D Structure for Q9BV86
Antigen Species
Human
Tag Information
His-tag

Product Categories/Family for NTMT1 recombinant protein

Cell Cycle
Applications Tested/Suitable for NTMT1 recombinant protein
SDS-PAGE

SDS-Page of NTMT1 recombinant protein

NTMT1 Recombinant Protein – Cat# MBS206159

Product References and Citations for NTMT1 recombinant protein

Petkowski JJ., et al. (2013) Biochem J. 456 (3): 453-462.; Richardson SL., et al. (2015) J Biol Chem. 290 (18):11601-10.

Other Notes

Small volumes of NTMT1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NTMT1 purchase

MBS206159 is a recombinant protein targeting NTMT1. Also known as N-terminal Xaa-Pro-Lys N-methyltransferase1 isoform a, 1-223aa, Human, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for NTMT1. It may not necessarily be applicable to this product.

NCBI GI #
56676399
NCBI GeneID
28989
NCBI Accession #
NP_054783
UniProt Primary Accession #
Q9BV86
UniProt Secondary Accession #
Q5SZB9; Q9UI28; A8K4J2; A8K8G7
UniProt Related Accession #
Q9BV86
GenBank Nucleotide #
NM_014064.3
NCBI Official Full Name
N-terminal Xaa-Pro-Lys N-methyltransferase 1 isoform a
NCBI Official Synonym Full Names
N-terminal Xaa-Pro-Lys N-methyltransferase 1
NCBI Official Symbol
NTMT1
NCBI Official Synonym Symbols
NRMT; NRMT1; NTM1A; AD-003; HOMT1A; C9orf32; METTL11A
UniProt Protein Name
N-terminal Xaa-Pro-Lys N-methyltransferase 1
UniProt Synonym Protein Names
Alpha N-terminal protein methyltransferase 1A; Methyltransferase-like protein 11A; N-terminal RCC1 methyltransferase; X-Pro-Lys N-terminal protein methyltransferase 1A; NTM1A
Protein Family
N-terminal Xaa-Pro-Lys N-methyltransferase
UniProt Gene Name
NTMT1
UniProt Synonym Gene Names
C9orf32; METTL11A; NRMT; NRMT1; NTM1A
NCBI Protein Information
N-terminal Xaa-Pro-Lys N-methyltransferase 1

NCBI Summary for NTMT1

The METTL11A gene encodes an N-terminal methyltransferase for the RAN (MIM 601179) guanine nucleotide exchange factor regulator of chromosome condensation 1 (RCC1; MIM 179710). METTL11A enzyme alpha-N-methylates other protein targets such as SET (MIM 600960) and RB (MIM 180200).[supplied by OMIM, Nov 2010]
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UniProt Comments for NTMT1

Distributive alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Gly/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. Specifically catalyzes mono-, di- or tri-methylation of the exposed alpha-amino group of the Ala, Gly or Ser residue in the [Ala/Gly/Ser]-Pro-Lys motif and mono- or di-methylation of Pro in the Pro-Pro-Lys motif. Some of the substrates may be primed by METTL11B-mediated monomethylation (PubMed:24090352). Catalyzes the trimethylation of the N-terminal Gly in CENPA (after removal of Met-1). Responsible for the N-terminal methylation of KLHL31, MYL2, MYL3, RB1, RCC1, RPL23A and SET. Required during mitosis for normal bipolar spindle formation and chromosome segregation via its action on RCC1.
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Research Articles on NTMT1

1. NRMT1 mutants N209I (endometrial cancer) and P211S (lung cancer) alter its catalytic activity and decrease N-terminal trimethylation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with NTMT1 recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Heart Antibodies  >1 publications with NTMT1 and Heart
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