NME2 Active Protein

Name: NME2 Active Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS206177
Price: 180 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS206177

NME2, also known as NM23B, is a heterodimeric protein functioning as a nucleoside diphosphate (NDP) kinase. NME1 and NME2 comprise the 152 amino acid A and B polypeptide chains of the NM23 enzyme, respectively. NME2 is identical to the beta subunit of human… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

NME2, Active Protein

Full Product Name

NME2, 1-152aa, Human, E Coli (Bioactivity Validated)

Product Gene Name

NME2 active protein

Product Synonym Names

Nucleoside diphosphate kinase B; NDPK-B; NDPKB; NM23-H2; NM23B

Sequence

MANLERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVAMKF LRASEEHLKQ HYIDLKDRPF FPGLVKYMNS GPVVAMVWEG LNVVKTGRVM LGETNPADSK PGTIRGDFCI QVGRNIIHGS DSVKSAEKEI SLWFKPEELV DYKSCAHDWV YE

Host

E Coli

Form/Format

Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Purity/Purification

> 90% by SDS-PAGE

Concentration

1 mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at 2-8 degree C short term
For long term storage, aliquot and store at -20 degree C or -80 degree C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

17.2 kDa (152aa), confirmed by MALDI-TOF

OMIM

X58965 mRNA

3D Structure

ModBase 3D Structure for P22392

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Domain

1-152aa

Biological Activity

Specific activity is > 1,800 units/mg, and is defined as the amount of enzyme that convert 1.0 umole each of ATP and TDP to ADP and TTP per minute at pH 7.5 at 25C in a couple system with PK/LDH.

Product Categories/Family for NME2 active protein

Metabolism; Signal Transduction; Cancer

Applications Tested/Suitable for NME2 active protein

SDS-PAGE, Enzyme Activity

Product References and Citations for NME2 active protein

Munkonge FM., et al. (2009) J Biol Chem. 284 (39):26978-87.; Treharne KJ., et al. (2009) FEBS Lett. 583 (17):2789-92.

Other Notes

Small volumes of NME2 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NME2 purchase

MBS206177 is an active protein targeting NME2. Also known as NME2, 1-152aa, Human, E Coli (Bioactivity Validated). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for NME2. It may not necessarily be applicable to this product.

NCBI GI #

66392227

NCBI GeneID

4831

NCBI Accession #

NP_001018149

UniProt Primary Accession #

P22392

UniProt Secondary Accession #

Q1WM23; Q6LCT6; A8MWA3

UniProt Related Accession #

P22392

GenBank Nucleotide #

NM_001018139.2

NCBI Official Full Name

nucleoside diphosphate kinase B isoform a

NCBI Official Synonym Full Names

NME/NM23 nucleoside diphosphate kinase 2

NCBI Official Symbol

NME2

NCBI Official Synonym Symbols

PUF; NDKB; NDPKB; NM23B; NDPK-B; NM23-H2

UniProt Protein Name

Nucleoside diphosphate kinase B

UniProt Synonym Protein Names

C-myc purine-binding transcription factor PUF; Histidine protein kinase NDKB (EC:2.7.13.3); nm23-H2

UniProt Gene Name

NME2

UniProt Synonym Gene Names

NM23B; NDK B; NDP kinase B

NCBI Protein Information

nucleoside diphosphate kinase B

NCBI Summary for NME2

Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by this gene) isoforms. Multiple alternatively spliced transcript variants have been found for this gene. Read-through transcription from the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) that encode a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Nov 2010]

UniProt Comments for NME2

Major role in the synthesis of nucleoside triphosphates other than ATP. Negatively regulates Rho activity by interacting with AKAP13/LBC. Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically (PubMed:8392752). Exhibits histidine protein kinase activity.

Research Articles on NME2

1. The data presented suggest an important role for cytoplasmic IRF6 in regulating the availability or localization of the NME1/2 complex and thus the dynamic behavior of epithelia during lip/palate development.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with NME2 active protein

Reference Product	PubMed Publications
NME1-NME2 active protein	>2 publications with NME2 and NME1-NME2

Pathways associated with NME2 active protein

Products by Pathway	Pathway Diagram
Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway antibodies	Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway Diagram
Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway antibodies	Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway Diagram
CMP Phosphorylation Pathway antibodies	CMP Phosphorylation Pathway Diagram
CMP Phosphorylation Pathway antibodies	CMP Phosphorylation Pathway Diagram
Guanine Ribonucleotide Biosynthesis IMP => GDP,GTP Pathway antibodies	Guanine Ribonucleotide Biosynthesis IMP => GDP,GTP Pathway Diagram
Guanine Ribonucleotide Biosynthesis IMP => GDP,GTP Pathway antibodies	Guanine Ribonucleotide Biosynthesis IMP => GDP,GTP Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Innate Immune System Pathway antibodies	Innate Immune System Pathway Diagram
Metabolic Pathways antibodies	Metabolic Pathways Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram

Diseases associated with NME2 active protein

Disease Name	Pubmed Publications
Neoplasm Metastasis Antibodies	>15 publications with NME2 and Neoplasm Metastasis
Disease Models, Animal Antibodies	>11 publications with NME2 and Disease Models, Animal
Lung Diseases Antibodies	>7 publications with NME2 and Lung Diseases
Breast Neoplasms Antibodies	>6 publications with NME2 and Breast Neoplasms
Lung Neoplasms Antibodies	>6 publications with NME2 and Lung Neoplasms
Liver Diseases Antibodies	>5 publications with NME2 and Liver Diseases
Neoplasms, Experimental Antibodies	>4 publications with NME2 and Neoplasms, Experimental
Carcinoma, Hepatocellular Antibodies	>3 publications with NME2 and Carcinoma, Hepatocellular
Ovarian Neoplasms Antibodies	>3 publications with NME2 and Ovarian Neoplasms
Mesothelioma Antibodies	>3 publications with NME2 and Mesothelioma

Organs/Tissues associated with NME2 active protein

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>15 publications with NME2 and Lung
Blood Antibodies	>15 publications with NME2 and Blood
Brain Antibodies	>14 publications with NME2 and Brain
Liver Antibodies	>9 publications with NME2 and Liver
Embryonic Tissue Antibodies	>7 publications with NME2 and Embryonic Tissue
Heart Antibodies	>7 publications with NME2 and Heart
Bone Marrow Antibodies	>6 publications with NME2 and Bone Marrow
Muscle Antibodies	>6 publications with NME2 and Muscle
Vascular Antibodies	>6 publications with NME2 and Vascular
Bone Antibodies	>6 publications with NME2 and Bone