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IdhA Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS206186

Idha, also known as D-lactate dehydrogenase, belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. In enzymology, a D-lactate dehydrogenase (cytochrome) is an enzyme that catalyzes the chemical reaction. Thus, the two substrates of this enzyme… More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
IdhA, Active Protein
Full Product Name
IdhA, 1-329aa, E Coli, His tag, E Coli (Bioactivity Validated)
Product Gene Name
Product Synonym Names
fermentative D-lactate dehydrogenase NAD-dependent; fermentative D-lactate dehydrogenase; NAD-dependent; ECK1377; hslF; hslI; htpH; JW1375
Sequence
MGSSHHHHHH SSGLVPRGSH MATLKEKLIA PVAEEEATVP NNKITVVGVG QVGMACAISI LGKSLADELA LVDVLEDKLK GEMMDLQHGS LFLQTPKIVA DKDYSVTANS KIVVVTAGVR QQEGESRLNL VQRNVNVFKF IIPQIVKYSP DCIIIVVSNP VDILTYVTWK LSGLPKHRVI GSGCNLDSAR FRYLMAEKLG IHPSSCHGWI LGEHGDSSVA VWSGVNVAGV SLQELNPEMG TDNDSENWKE VHKMVVESAY EVIKLKGYTN WAIGLSVADL IESMLKNLSR IHPVSTMVKG MYGIENEVFL SLPCILNARG LTSVINQKLK DDEVAQLKKS ADTLWDIQKD LKDL
Host
E Coli
Form/Format
Liquid. In 20 mM Tris-HCl Buffer, pH8.0, 10% glycerol, 100mM NaCl
Purity/Purification
> 95% by SDS-PAGE
Concentration
1 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
39.1kDa (353aa), confirmed by MALDI-TOF
3D Structure
ModBase 3D Structure for P52643
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Antigen Species
E Coli
Tag Information
His-tag
Biological Activity
Specific activity is > 200 units/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37C.

Product Categories/Family for IdhA active protein

Applications Tested/Suitable for IdhA active protein
SDS-PAGE

Product References and Citations for IdhA active protein

Atlante, A., et al. (2005) Biochim. Biophys. Acta 1708 (1): 13-22.; Martin Engqvist, et al. (2009) J Biol Chem. 284 (September 11): 25026-25037.

Other Notes

Small volumes of IdhA active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IdhA purchase

MBS206186 is an active protein targeting IdhA. Also known as IdhA, 1-329aa, E Coli, His tag, E Coli (Bioactivity Validated). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for IdhA. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
GenBank Nucleotide #
NCBI Official Full Name
fermentative D-lactate dehydrogenase, NAD-dependent
NCBI Official Symbol
ldhA
NCBI Official Synonym Symbols
ECK1377; hslF; hslI; htpH; JW1375
UniProt Protein Name
D-lactate dehydrogenase
UniProt Synonym Protein Names
Fermentative lactate dehydrogenase
UniProt Gene Name
ldhA
UniProt Synonym Gene Names
hslI; htpH; D-LDH
NCBI Protein Information
fermentative D-lactate dehydrogenase, NAD-dependent

NCBI Summary for IdhA

There are three lactate dehydrogenase enzymes in Escherichia coli which interconvert pyruvate and lactate. [More information is available at EcoCyc: G592].

UniProt Comments for IdhA

Fermentative lactate dehydrogenase.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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