3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

PDIA4 Active Protein

Name: PDIA4 Active Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS206335
Price: 245 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS206335

PDIA4, also known as ERP72, is an endoplasmic reticulum luminal protein that is both a stress protein and a member of the protein disulfide isomerase family of proteins. It is involved in the catalysis of protein-S-S-bond rearrangement. PDIA3 and PDIA4 act as… More details

$245 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

PDIA4, Active Protein

Full Product Name

PDIA4, 21-645aa, Human, His tag, E Coli (Bioactivity Validated)

Product Gene Name

PDIA4 active protein

Product Synonym Names

Protein disulfide-isomerase A4; Endoplasmic reticulum resident protein 72; ERP70; ERP72

Sequence

MGSSHHHHHH SSGLVPRGSH MVAGAEGPDE DSSNRENAIE DEEEEEEEDD DEEEDDLEVK EENGVLVLND ANFDNFVADK DTVLLEFYAP WCGHCKQFAP EYEKIANILK DKDPPIPVAK IDATSASVLA SRFDVSGYPT IKILKKGQAV DYEGSRTQEE IVAKVREVSQ PDWTPPPEVT LVLTKENFDE VVNDADIILV EFYAPWCGHC KKLAPEYEKA AKELSKRSPP IPLAKVDATA ETDLAKRFDV SGYPTLKIFR KGRPYDYNGP REKYGIVDYM IEQSGPPSKE ILTLKQVQEF LKDGDDVIII GVFKGESDPA YQQYQDAANN LREDYKFHHT FSTEIAKFLK VSQGQLVVMQ PEKFQSKYEP RSHMMDVQGS TQDSAIKDFV LKYALPLVGH RKVSNDAKRY TRRPLVVVYY SVDFSFDYRA ATQFWRSKVL EVAKDFPEYT FAIADEEDYA GEVKDLGLSE SGEDVNAAIL DESGKKFAME PEEFDSDTLR EFVTAFKKGK LKPVIKSQPV PKNNKGPVKV VVGKTFDSIV MDPKKDVLIE FYAPWCGHCK QLEPVYNSLA KKYKGQKGLV IAKMDATAND VPSDRYKVEG FPTIYFAPSG DKKNPVKFEG GDRDLEHLSK FIEEHATKLS RTKEEL

Host

E Coli

Form/Format

Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 0.1 M NaCl, 1mM DTT and 10% glycerol

Purity/Purification

> 90% by SDS-PAGE

Concentration

1 mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at 2-8 degree C short term
For long term storage, aliquot and store at -20 degree C or -80 degree C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

72.9 kDa (646aa), confirmed by MALDI-TOF

3D Structure

ModBase 3D Structure for P13667

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Antigen Species

Human

Tag Information

His-tag

Endotoxin level

< 1 EU per 1ug of protein (determined by LAL method)

Biological Activity

Specific activity > 5 A650/cm/min/mg. Enzymatic activity was confirmed by measuring the aggregation of insulin in the presence of DTT

Product Categories/Family for PDIA4 active protein

Heat Shock Proteins (chaperonins)

Applications Tested/Suitable for PDIA4 active protein

SDS-PAGE, Enzyme Activity

Product References and Citations for PDIA4 active protein

Thomas M., et al. (2010) Synapse. 64 (8):579-93.; Joo JH., et al. (2007) Cancer Res. 67 (16):7929-36.

Other Notes

Small volumes of PDIA4 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PDIA4 purchase

MBS206335 is an active protein targeting PDIA4. Also known as PDIA4, 21-645aa, Human, His tag, E Coli (Bioactivity Validated). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for PDIA4. It may not necessarily be applicable to this product.

NCBI GI #

4758304

NCBI GeneID

9601

NCBI Accession #

NP_004902

UniProt Primary Accession #

P13667

UniProt Secondary Accession #

Q549T6; A8K4K6

UniProt Related Accession #

P13667

GenBank Nucleotide #

NM_004911.4

NCBI Official Full Name

protein disulfide-isomerase A4

NCBI Official Synonym Full Names

protein disulfide isomerase family A member 4

NCBI Official Symbol

PDIA4

NCBI Official Synonym Symbols

ERP70; ERP72; ERp-72

UniProt Protein Name

Protein disulfide-isomerase A4

UniProt Synonym Protein Names

Endoplasmic reticulum resident protein 70; ER protein 70; ERp70; Endoplasmic reticulum resident protein 72; ER protein 72; ERp-72; ERp72

Protein Family

Protein disulfide-isomerase

UniProt Gene Name

PDIA4

UniProt Synonym Gene Names

ERP70; ERP72; ER protein 70; ERp70; ER protein 72; ERp-72; ERp72

NCBI Protein Information

protein disulfide-isomerase A4

NCBI Summary for PDIA4

This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, three catalytically active thioredoxin (TRX) domains, two TRX-like domains and a C-terminal ER-retention sequence. This protein, when bound to cyclophilin B, enhances the rate of immunoglobulin G intermolecular disulfide bonding and antibody assembly. [provided by RefSeq, Dec 2016]

Research Articles on PDIA4

1. PDIA4 as a negative regulator of cancer cell apoptosis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PDIA4 active protein

Reference Product	PubMed Publications
HSPA5 active protein	>5 publications with PDIA4 and HSPA5
CALR active protein	>2 publications with PDIA4 and CALR
ERO1LB active protein	>2 publications with PDIA4 and ERO1LB
HYOU1 active protein	>1 publications with PDIA4 and HYOU1

Pathways associated with PDIA4 active protein

Products by Pathway	Pathway Diagram
Protein Processing In Endoplasmic Reticulum Pathway antibodies	Protein Processing In Endoplasmic Reticulum Pathway Diagram
Protein Processing In Endoplasmic Reticulum Pathway antibodies	Protein Processing In Endoplasmic Reticulum Pathway Diagram
Thyroid Hormone Synthesis Pathway antibodies	Thyroid Hormone Synthesis Pathway Diagram
Thyroid Hormone Synthesis Pathway antibodies	Thyroid Hormone Synthesis Pathway Diagram
Vibrio Cholerae Infection Pathway antibodies	Vibrio Cholerae Infection Pathway Diagram
Vibrio Cholerae Infection Pathway antibodies	Vibrio Cholerae Infection Pathway Diagram

Diseases associated with PDIA4 active protein

Disease Name	Pubmed Publications
Inflammation Antibodies	>4 publications with PDIA4 and Inflammation
Lung Diseases Antibodies	>2 publications with PDIA4 and Lung Diseases
Liver Diseases Antibodies	>2 publications with PDIA4 and Liver Diseases
Abnormalities, Drug-Induced Antibodies	>1 publications with PDIA4 and Abnormalities, Drug-Induced
Carcinoma, Hepatocellular Antibodies	>1 publications with PDIA4 and Carcinoma, Hepatocellular
Nervous System Diseases Antibodies	>1 publications with PDIA4 and Nervous System Diseases
Liver Neoplasms Antibodies	>1 publications with PDIA4 and Liver Neoplasms

Organs/Tissues associated with PDIA4 active protein

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>4 publications with PDIA4 and Lung
Liver Antibodies	>3 publications with PDIA4 and Liver
Brain Antibodies	>3 publications with PDIA4 and Brain
Muscle Antibodies	>2 publications with PDIA4 and Muscle
Pancreas Antibodies	>2 publications with PDIA4 and Pancreas
Eye Antibodies	>2 publications with PDIA4 and Eye
Blood Antibodies	>2 publications with PDIA4 and Blood
Intestine Antibodies	>2 publications with PDIA4 and Intestine
Placenta Antibodies	>1 publications with PDIA4 and Placenta
Connective Tissue Antibodies	>1 publications with PDIA4 and Connective Tissue