PDIA6 Active Protein

Name: PDIA6 Active Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS206344
Price: 180 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS206344

PDIA6 is a member of the protein disulfide isomerase (PDI). PDI is an enzyme in the endoplasmic reticulum in eukaryotes or periplasmic space of prokaryotes that catalyzes the formation and breakage of disulfide bonds between cysteine residues within proteins… More details

$180 / 0.004 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

PDIA6, Active Protein

Full Product Name

PDIA6, 20-440aa, Human, His tag, E Coli (Bioactivity Validated)

Product Gene Name

PDIA6 active protein

Product Synonym Names

Protein disulfide-isomerase A6; ERP5; P5; TXNDC7

Sequence

MGSSHHHHHH SSGLVPRGSH MLYSSSDDVI ELTPSNFNRE VIQSDSLWLV EFYAPWCGHC QRLTPEWKKA ATALKDVVKV GAVDADKHHS LGGQYGVQGF PTIKIFGSNK NRPEDYQGGR TGEAIVDAAL SALRQLVKDR LGGRSGGYSS GKQGRSDSSS KKDVIELTDD SFDKNVLDSE DVWMVEFYAP WCGHCKNLEP EWAAAASEVK EQTKGKVKLA AVDATVNQVL ASRYGIRGFP TIKIFQKGES PVDYDGGRTR SDIVSRALDL FSDNAPPPEL LEIINEDIAK RTCEEHQLCV VAVLPHILDT GAAGRNSYLE VLLKLADKYK KKMWGWLWTE AGAQSELETA LGIGGFGYPA MAAINARKMK FALLKGSFSE QGINEFLREL SFGRGSTAPV GGGAFPTIVE REPWDGRDGE LPVEDDIDLS DVELDDLGKD EL

Host

E Coli

Form/Format

Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing, 50mM NaCl, 2mM DTT and 10% glycerol

Purity/Purification

> 90% by SDS-PAGE

Concentration

1 mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at 2-8 degree C short term
For long term storage, aliquot and store at -20 degree C or -80 degree C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

48.5 kDa (442aa), confirmed by MALDI-TOF

OMIM

D49489 mRNA

3D Structure

ModBase 3D Structure for Q15084

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Tag Information

His-tag

Biological Activity

Specific activity > 20 A650/cm/min/mg. Enzymatic activity was confirmed by measuring the aggregation of insulin in the presence of DTT.

Product Categories/Family for PDIA6 active protein

Heat Shock Proteins (chaperonins)

Applications Tested/Suitable for PDIA6 active protein

SDS-PAGE, Enzyme Activity

Product References and Citations for PDIA6 active protein

Jordan P.A., et al. (2005) Blood. 105:1500-1507; Kikuchi M., et al. (2002) J. Biochem. 132:451-455

Other Notes

Small volumes of PDIA6 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PDIA6 purchase

MBS206344 is an active protein targeting PDIA6. Also known as PDIA6, 20-440aa, Human, His tag, E Coli (Bioactivity Validated). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for PDIA6. It may not necessarily be applicable to this product.

NCBI GI #

5031973

NCBI GeneID

10130

NCBI Accession #

NP_005733

UniProt Primary Accession #

Q15084

UniProt Secondary Accession #

Q53RC7; Q6ZSH5; Q99778; B3KY95; B5MCQ5; B7Z254; B7Z4M8; F8WA83

UniProt Related Accession #

Q15084

GenBank Nucleotide #

NM_005742.3

NCBI Official Full Name

protein disulfide-isomerase A6 isoform d

NCBI Official Synonym Full Names

protein disulfide isomerase family A member 6

NCBI Official Symbol

PDIA6

NCBI Official Synonym Symbols

P5; ERP5; TXNDC7

UniProt Protein Name

Protein disulfide-isomerase A6

UniProt Synonym Protein Names

Endoplasmic reticulum protein 5; ER protein 5; ERp5; Protein disulfide isomerase P5; Thioredoxin domain-containing protein 7

Protein Family

Protein disulfide-isomerase

UniProt Gene Name

PDIA6

UniProt Synonym Gene Names

ERP5; P5; TXNDC7; ER protein 5; ERp5

NCBI Protein Information

protein disulfide-isomerase A6

NCBI Summary for PDIA6

This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, two catalytically active thioredoxin (TRX) domains, a TRX-like domain, and a C-terminal ER-retention sequence. This protein inhibits the aggregation of misfolded proteins and exhibits both isomerase and chaperone activity. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2016]

UniProt Comments for PDIA6

May function as a chaperone that inhibits aggregation of misfolded proteins (PubMed:12204115). Negatively regulates the unfolded protein response (UPR) through binding to UPR sensors such as ERN1, which in turn inactivates ERN1 signaling (PubMed:24508390). May also regulate the UPR via the EIF2AK3 UPR sensor (PubMed:24508390). Plays a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin (PubMed:15466936).

Research Articles on PDIA6

1. Results indicate that protein disulfide isomerase family 6 (PDIA6) overexpression promoted the proliferation of HeLa cells by suppressing the phosphorylation of beta-catenin, thereby inhibiting the degradation of beta-catenin through the ubiquitin-proteasome pathway.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PDIA6 active protein

Reference Product	PubMed Publications
HSPA5 active protein	>6 publications with PDIA6 and HSPA5
P4HB active protein	>2 publications with PDIA6 and P4HB
HSP90B1 active protein	>2 publications with PDIA6 and HSP90B1

Pathways associated with PDIA6 active protein

Products by Pathway	Pathway Diagram
IRE1alpha Activates Chaperones Pathway antibodies	IRE1alpha Activates Chaperones Pathway Diagram
Metabolism Of Proteins Pathway antibodies	Metabolism Of Proteins Pathway Diagram
Photodynamic Therapy-induced Unfolded Protein Response Pathway antibodies	Photodynamic Therapy-induced Unfolded Protein Response Pathway Diagram
Protein Processing In Endoplasmic Reticulum Pathway antibodies	Protein Processing In Endoplasmic Reticulum Pathway Diagram
Protein Processing In Endoplasmic Reticulum Pathway antibodies	Protein Processing In Endoplasmic Reticulum Pathway Diagram
Unfolded Protein Response (UPR) Pathway antibodies	Unfolded Protein Response (UPR) Pathway Diagram
XBP1(S) Activates Chaperone Genes Pathway antibodies	XBP1(S) Activates Chaperone Genes Pathway Diagram

Diseases associated with PDIA6 active protein

Disease Name	Pubmed Publications
Death Antibodies	>6 publications with PDIA6 and Death
Liver Diseases Antibodies	>3 publications with PDIA6 and Liver Diseases
Brain Diseases Antibodies	>3 publications with PDIA6 and Brain Diseases
Lung Diseases Antibodies	>3 publications with PDIA6 and Lung Diseases
Disease Models, Animal Antibodies	>2 publications with PDIA6 and Disease Models, Animal
Cell Transformation, Neoplastic Antibodies	>2 publications with PDIA6 and Cell Transformation, Neoplastic
Kidney Diseases Antibodies	>2 publications with PDIA6 and Kidney Diseases
Inflammation Antibodies	>2 publications with PDIA6 and Inflammation
Carcinoma, Hepatocellular Antibodies	>1 publications with PDIA6 and Carcinoma, Hepatocellular
Cardiovascular Diseases Antibodies	>1 publications with PDIA6 and Cardiovascular Diseases

Organs/Tissues associated with PDIA6 active protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>7 publications with PDIA6 and Blood
Brain Antibodies	>7 publications with PDIA6 and Brain
Lung Antibodies	>6 publications with PDIA6 and Lung
Pancreas Antibodies	>4 publications with PDIA6 and Pancreas
Bone Antibodies	>4 publications with PDIA6 and Bone
Liver Antibodies	>4 publications with PDIA6 and Liver
Kidney Antibodies	>3 publications with PDIA6 and Kidney
Skin Antibodies	>2 publications with PDIA6 and Skin
Intestine Antibodies	>2 publications with PDIA6 and Intestine
Lymph Node Antibodies	>2 publications with PDIA6 and Lymph Node