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UBA2 Baculovirus Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS206369

UBA2, also known as SUMO-activating enzyme subunit 2, is a family of small and related proteins that can be enzymatically attached to a target protein by a post-translational modification process termed sumoylation. It is conjugated to a variety of molecules… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
UBA2, Recombinant Protein
Full Product Name
UBA2, 1-640aa, Human, His tag, Baculovirus
Product Gene Name
Product Synonym Names
SUMO-activating enzyme subunit 2; UBA2; ARX; HRIHFB2115; SAE2
Sequence
ADLMALSRGL PRELAEAVAG GRVLVVGAGG IGCELLKNLV LTGFSHIDLI DLDTIDVSNL NRQFLFQKKH VGRSKAQVAK ESVLQFYPKA NIVAYHDSIM NPDYNVEFFR QFILVMNALD NRAARNHVNR MCLAADVPLI ESGTAGYLGQ VTTIKKGVTE CYECHPKPTQ RTFPGCTIRN TPSEPIHCIV WAKYLFNQLF GEEDADQEVS PDRADPEAAW EPTEAEARAR ASNEDGDIKR ISTKEWAKST GYDPVKLFTK LFKDDIRYLL TMDKLWRKRK PPVPLDWAEV QSQGEETNAS DQQNEPQLGL KDQQVLDVKS YARLFSKSIE TLRVHLAEKG DGAELIWDKD DPSAMDFVTS AANLRMHIFS MNMKSRFDIK SMAGNIIPAI ATTNAVIAGL IVLEGLKILS GKIDQCRTIF LNKQPNPRKK LLVPCALDPP NPNCYVCASK PEVTVRLNVH KVTVLTLQDK IVKEKFAMVA PDVQIEDGKG TILISSEEGE TEANNHKKLS EFGIRNGSRL QADDFLQDYT LLINILHSED LGKDVEFEVV GDAPEKVGPK QAEDAAKSIT NGSDDGAQPS TSTAQEQDDV LIVDSDEEDS SNNADVSEEE RSRKRKLDEK ENLSAKRSRI EQKEELDDVI ALDHHHHHH
Host
Baculovirus
Form/Format
Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
Purity/Purification
> 85% by SDS-PAGE
Concentration
0.5 mg/ml (determined by Absorbance at 280nm) (lot specific)
Preparation and Storage
Can be stored at 2°C to 8°C for one week.
For long term storage, aliquot and store at -20°C to -80°C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
72.3kDa (649aa)
OMIM
AF090384 mRNA
3D Structure
ModBase 3D Structure for Q9UBT2
Tag Information
His-tag
Domain
1-640aa
Endotoxin Level
< 1.0 EU per 1 ug of protein (determined by LAL method)

Product Categories/Family for UBA2 recombinant protein

Applications Tested/Suitable for UBA2 recombinant protein
SDS-PAGE

SDS-Page of UBA2 recombinant protein

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Baculovirus UBA2 Recombinant Protein – Cat# MBS206369

Product References and Citations for UBA2 recombinant protein

Gong L., et al. (1999) FEBS Lett. 448:185-189.; Tatham MH., et al. (2001) J Biol Chem. 276:35368-35374.

Other Notes

Small volumes of UBA2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for UBA2 purchase

MBS206369 is a recombinant protein targeting UBA2. Also known as UBA2, 1-640aa, Human, His tag, Baculovirus. Host: Baculovirus.

Additional Details

NCBI/Uniprot data below describe general gene information for UBA2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
SUMO-activating enzyme subunit 2
NCBI Official Synonym Full Names
ubiquitin like modifier activating enzyme 2
NCBI Official Symbol
UBA2
NCBI Official Synonym Symbols
ARX; SAE2; HRIHFB2115
UniProt Protein Name
SUMO-activating enzyme subunit 2
UniProt Synonym Protein Names
Anthracycline-associated resistance ARX; Ubiquitin-like 1-activating enzyme E1B; Ubiquitin-like modifier-activating enzyme 2
UniProt Gene Name
UBA2
UniProt Synonym Gene Names
SAE2; UBLE1B
NCBI Protein Information
SUMO-activating enzyme subunit 2

NCBI Summary for UBA2

Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 (MIM 613294) and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010]
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UniProt Comments for UBA2

The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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