Baculovirus UCHL3 Active Protein – Cat# MBS206419

UCHL3 Baculovirus Active Protein

Name: UCHL3 Active Protein; Baculovirus; MyBioSource
Brand: MyBioSource
SKU: MBS206419
Price: 245 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS206419

Uchl3, as known as ubiquitin carboxyl-terminal hydrolase isozyme L3, is a member of the peptidase C12 family of deubiquitinating enzymes. It is widely expressed with the highest levels being observed in heart, testis, thymus and striated muscle. This protein… More details

$245 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Uchl3, Active Protein

Full Product Name

Uchl3, 1-230aa, Mouse, His tag, Baculovirus (Bioactivity Validated)

Product Gene Name

Uchl3 active protein

Product Synonym Names

Ubiquitin carboxyl-terminal hydrolase isozyme L3; Uchl3; Ubiquitin carboxyl-terminal esterase L3; Ubiquitin thioesterase L3; Ubiquitin thiolesterase; Ubiquitin thiolesterase L3; UCH L3; UCH-L3; UCHL3; UCHL3_HUMAN

Sequence

MEGQRWLPLE ANPEVTNQFL KQLGLHPNWQ FVDVYGMEPE LLSMVPRPVC AVLLLFPITE KYEVFRTEEE EKIKSQGQDV TSSVYFMKQT ISNACGTIGL IHAIANNKDK MHFESGSTLK KFLEESVSMS PEERAKFLEN YDAIRVTHET SAHEGQTEAP SIDEKVDLHF IALVHVDGHL YELDGRKPFP INHGKTSDET LLEDAIEVCK KFMERDPDEL RFNAIALSAA LEHHHHHH

Host

Baculovirus

Form/Format

Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.

Purity/Purification

> 90% by SDS-PAGE

Concentration

0.5 mg/ml (determined by Absorbance at 280nm) (lot specific)

Preparation and Storage

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

27.2 kDa (238aa) 28-40kDa (SDS-PAGE under reducing conditions)

3D Structure

ModBase 3D Structure for Q9JKB1

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Antigen Species

Mouse

Tag Information

His-tag

Endotoxin Level

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Biological Activity

Specific activity is > 9,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolysis 1.0 pmole of ubiquitin-AMC per minute at pH 7.5, at 37C.

Product Categories/Family for Uchl3 active protein

Ubiquitination

Applications Tested/Suitable for Uchl3 active protein

SDS-PAGE

Product References and Citations for Uchl3 active protein

Mtango NR., et al. (2012) J. Cell. Physiol. 227:2022-2029.; Dennissen FJ., et al. (2011) FEBS Lett. 585:2568-2574.

Other Notes

Small volumes of Uchl3 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Uchl3 purchase

MBS206419 is an active protein targeting Uchl3. Also known as Uchl3, 1-230aa, Mouse, His tag, Baculovirus (Bioactivity Validated). Host: Baculovirus.

Additional Details

NCBI/Uniprot data below describe general gene information for Uchl3. It may not necessarily be applicable to this product.

NCBI GI #

139948802

NCBI GeneID

50933

NCBI Accession #

NP_057932

UniProt Primary Accession #

Q9JKB1

UniProt Secondary Accession #

Q9EQX7

UniProt Related Accession #

Q9JKB1

GenBank Nucleotide #

NM_016723.2

NCBI Official Full Name

ubiquitin carboxyl-terminal hydrolase isozyme L3

NCBI Official Synonym Full Names

ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)

NCBI Official Symbol

Uchl3

UniProt Protein Name

Ubiquitin carboxyl-terminal hydrolase isozyme L3

UniProt Synonym Protein Names

Ubiquitin thioesterase L3

Protein Family

Ubiquitin carboxyl-terminal hydrolase

UniProt Gene Name

Uchl3

UniProt Synonym Gene Names

UCH-L3

NCBI Protein Information

ubiquitin carboxyl-terminal hydrolase isozyme L3

UniProt Comments for Uchl3

Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3", and exhibits a preference towards 'Lys-48'-linked ubiquitin chains. Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. Required for stress-response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory. Can hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome.

Research Articles on Uchl3

1. Data suggest that the activity of oocyte UCHL-1 and -3 contributes to oocyte maturation by regulating the oocyte cortex and meiotic spindle.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with Uchl3 active protein

Reference Product	PubMed Publications
Uchl1 active protein	>18 publications with Uchl3 and Uchl1
Nedd8 active protein	>3 publications with Uchl3 and Nedd8
Usp2 active protein	>1 publications with Uchl3 and Usp2
Usp14 active protein	>1 publications with Uchl3 and Usp14
Uchl4 active protein	>1 publications with Uchl3 and Uchl4
Ubc active protein	>1 publications with Uchl3 and Ubc

Pathways associated with Uchl3 active protein

Products by Pathway	Pathway Diagram
Deubiquitination Pathway antibodies	Deubiquitination Pathway Diagram
Metabolism Of Proteins Pathway antibodies	Metabolism Of Proteins Pathway Diagram
Post-translational Protein Modification Pathway antibodies	Post-translational Protein Modification Pathway Diagram
Proteasome Degradation Pathway antibodies	Proteasome Degradation Pathway Diagram
Protein Ubiquitination Pathway antibodies	Protein Ubiquitination Pathway Diagram
Synthesis Of Active Ubiquitin: Roles Of E1 And E2 Enzymes Pathway antibodies	Synthesis Of Active Ubiquitin: Roles Of E1 And E2 Enzymes Pathway Diagram
UCH Proteinases Pathway antibodies	UCH Proteinases Pathway Diagram

Diseases associated with Uchl3 active protein

Disease Name	Pubmed Publications
Disease Models, Animal Antibodies	>3 publications with Uchl3 and Disease Models, Animal
Liver Diseases Antibodies	>2 publications with Uchl3 and Liver Diseases
Insulin Resistance Antibodies	>1 publications with Uchl3 and Insulin Resistance
Cell Transformation, Neoplastic Antibodies	>1 publications with Uchl3 and Cell Transformation, Neoplastic
Carcinoma, Hepatocellular Antibodies	>1 publications with Uchl3 and Carcinoma, Hepatocellular
Liver Neoplasms Antibodies	>1 publications with Uchl3 and Liver Neoplasms
Learning Disorders Antibodies	>1 publications with Uchl3 and Learning Disorders
Memory Disorders Antibodies	>1 publications with Uchl3 and Memory Disorders
Hyperplasia Antibodies	>1 publications with Uchl3 and Hyperplasia
Cognition Disorders Antibodies	>1 publications with Uchl3 and Cognition Disorders

Organs/Tissues associated with Uchl3 active protein

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>8 publications with Uchl3 and Brain
Liver Antibodies	>4 publications with Uchl3 and Liver
Testis Antibodies	>4 publications with Uchl3 and Testis
Embryonic Tissue Antibodies	>4 publications with Uchl3 and Embryonic Tissue
Lung Antibodies	>1 publications with Uchl3 and Lung
Ovary Antibodies	>1 publications with Uchl3 and Ovary
Eye Antibodies	>1 publications with Uchl3 and Eye