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IDO1 Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS206431

IDO1 is an indoleamine 2,3-dioxygenase - a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, and serotonin. It is thought to… More details

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Product Overview

Online Datasheet in PDF
Product Name
IDO1, Active Protein
Full Product Name
IDO1, 1-403aa, Human, His-tag, E Coli (Bioactivity Validated)
Product Gene Name
IDO1 active protein
Product Synonym Names
Indoleamine 2,3-dioxygenase 1; IDO; IDO-1; INDO
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAHAMEN SWTISKEYHI DEEVGFALPN PQENLPDFYN DWMFIAKHLP DLIESGQLRE RVEKLNMLSI DHLTDHKSQR LARLVLGCIT MAYVWGKGHG DVRKVLPRNI AVPYCQLSKK LELPPILVYA DCVLANWKKK DPNKPLTYEN MDVLFSFRDG DCSKGFFLVS LLVEIAAASA IKVIPTVFKA MQMQERDTLL KALLEIASCL EKALQVFHQI HDHVNPKAFF SVLRIYLSGW KGNPQLSDGL VYEGFWEDPK EFAGGSAGQS SVFQCFDVLL GIQQTAGGGH AAQFLQDMRR YMPPAHRNFL CSLESNPSVR EFVLSKGDAG LREAYDACVK ALVSLRSYHL QIVTKYILIP ASQQPKENKT SEDPSKLEAK GTGGTDLMNF LKTVRSTTEK SLLKEG
Host
E Coli
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
Purity/Purification
> 90% by SDS-PAGE
Concentration
0.5 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
47.7 kDa (426aa), confirmed by MALDI-TOF
OMIM
M34455 mRNA
3D Structure
ModBase 3D Structure for P14902
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Tag Information
His-tag
Endotoxin Level
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Biological Activity
Specific activity is > 300 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of L-Tryptophan to N-formyl-L-kynurenine per minute at pH 6.5 at 25C.

Product Categories/Family for IDO1 active protein

Metabolism
Applications Tested/Suitable for IDO1 active protein
Enzyme Activity, SDS-PAGE

SDS-Page of IDO1 active protein

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
IDO1 Active Protein – Cat# MBS206431

Product References and Citations for IDO1 active protein

Metz R., et al (2007). Nature Cancer Res. 67:7082-7087; Yuasa H.J., et al (2007). J. Mol. Evol. 65:705-714

Other Notes

Small volumes of IDO1 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IDO1 purchase

MBS206431 is an active protein targeting IDO1. Also known as IDO1, 1-403aa, Human, His-tag, E Coli (Bioactivity Validated). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for IDO1. It may not necessarily be applicable to this product.

NCBI GI #
4504577
NCBI GeneID
3620
NCBI Accession #
NP_002155
UniProt Primary Accession #
P14902
UniProt Secondary Accession #
Q540B4; E5RGR8; F6M9T7
UniProt Related Accession #
P14902
GenBank Nucleotide #
NM_002164.5
NCBI Official Full Name
indoleamine 2,3-dioxygenase 1
NCBI Official Synonym Full Names
indoleamine 2,3-dioxygenase 1
NCBI Official Symbol
IDO1
NCBI Official Synonym Symbols
IDO; INDO; IDO-1
UniProt Protein Name
Indoleamine 2,3-dioxygenase 1
UniProt Synonym Protein Names
Indoleamine-pyrrole 2,3-dioxygenase
Protein Family
Indoleamine 2,3-dioxygenase
UniProt Gene Name
IDO1
UniProt Synonym Gene Names
IDO; INDO; IDO-1
NCBI Protein Information
indoleamine 2,3-dioxygenase 1

NCBI Summary for IDO1

This gene encodes indoleamine 2,3-dioxygenase (IDO) - a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan.[provided by RefSeq, Feb 2011]
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UniProt Comments for IDO1

Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway (PubMed:17671174). Involved in the peripheral immune tolerance, contributing to maintain homeostasis by preventing autoimmunity or immunopathology that would result from uncontrolled and overreacting immune responses (PubMed:25691885). Tryptophan shortage inhibits T lymphocytes division and accumulation of tryptophan catabolites induces T-cell apoptosis and differentiation of regulatory T-cells (PubMed:25691885). Acts as a suppressor of anti-tumor immunity (PubMed:23103127, PubMed:25157255, PubMed:14502282, PubMed:25691885). Limits the growth of intracellular pathogens by depriving tryptophan (PubMed:25691885). Protects the fetus from maternal immune rejection (PubMed:25691885).
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Research Articles on IDO1

1. PD-L1, IDO-1, and B7-H4 are differentially expressed in human lung carcinomas and show limited co-expression. While PD-L1 and IDO-1 are associated with increased tumor-infiltrating lymphoycte and IFN-gamma stimulation, B7-H4 is not.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with IDO1 active protein

 Reference Product  PubMed Publications
 IDO2 active protein  >72 publications with IDO1 and IDO2
 TDO2 active protein  >24 publications with IDO1 and TDO2
 CYP1A1 active protein  >5 publications with IDO1 and CYP1A1
 KYNU active protein  >5 publications with IDO1 and KYNU
 TPH1 active protein  >2 publications with IDO1 and TPH1
 TPH2 active protein  >1 publications with IDO1 and TPH2
 CYP1B1 active protein  >1 publications with IDO1 and CYP1B1

Pathways associated with IDO1 active protein

 Products by Pathway  Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 Histidine, Lysine, Phenylalanine, Tyrosine, Proline And Tryptophan Catabolism Pathway antibodies  Histidine, Lysine, Phenylalanine, Tyrosine, Proline And Tryptophan Catabolism Pathway Diagram
 L-kynurenine Degradation Pathway antibodies  L-kynurenine Degradation Pathway Diagram
 L-tryptophan Degradation III (eukaryotic) Pathway antibodies  L-tryptophan Degradation III (eukaryotic) Pathway Diagram
 L-tryptophan Degradation XI (mammalian, Via Kynurenine) Pathway antibodies  L-tryptophan Degradation XI (mammalian, Via Kynurenine) Pathway Diagram
 L-tryptophan Degradation To 2-amino-3-carboxymuconate Semialdehyde Pathway antibodies  L-tryptophan Degradation To 2-amino-3-carboxymuconate Semialdehyde Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Amino Acids And Derivatives Pathway antibodies  Metabolism Of Amino Acids And Derivatives Pathway Diagram

Diseases associated with IDO1 active protein

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >101 publications with IDO1 and Inflammation
 Disease Models, Animal Antibodies  >42 publications with IDO1 and Disease Models, Animal
 Liver Diseases Antibodies  >19 publications with IDO1 and Liver Diseases
 Neoplasms, Experimental Antibodies  >18 publications with IDO1 and Neoplasms, Experimental
 Lung Diseases Antibodies  >13 publications with IDO1 and Lung Diseases
 Necrosis Antibodies  >12 publications with IDO1 and Necrosis
 Breast Neoplasms Antibodies  >11 publications with IDO1 and Breast Neoplasms
 Neurodegenerative Diseases Antibodies  >8 publications with IDO1 and Neurodegenerative Diseases
 Endometriosis Antibodies  >7 publications with IDO1 and Endometriosis
 Obesity Antibodies  >7 publications with IDO1 and Obesity

Organs/Tissues associated with IDO1 active protein

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >55 publications with IDO1 and Brain
 Lung Antibodies  >33 publications with IDO1 and Lung
 Liver Antibodies  >26 publications with IDO1 and Liver
 Bone Antibodies  >23 publications with IDO1 and Bone
 Kidney Antibodies  >17 publications with IDO1 and Kidney
 Intestine Antibodies  >16 publications with IDO1 and Intestine
 Uterus Antibodies  >8 publications with IDO1 and Uterus
 Placenta Antibodies  >8 publications with IDO1 and Placenta
 Muscle Antibodies  >6 publications with IDO1 and Muscle
 Heart Antibodies  >6 publications with IDO1 and Heart
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