Baculovirus MMP14 Recombinant Protein – Cat# MBS206467

MMP14 Baculovirus Recombinant Protein

Name: MMP14 Recombinant Protein; Baculovirus; MyBioSource
Brand: MyBioSource
SKU: MBS206467
Price: 375 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS206467

MMP14, also known as matrix metalloproteinase-14, is a membrane-anchored zinc-binding endopeptidase that is expressed at the leading edge of various invasive carcinomas and promotes tumor cell invasion through degradation of the extracellular matrix. It plays… More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

MMP14, Recombinant Protein

Full Product Name

MMP14, 21-538aa, Human, His-tag, Baculovirus

Product Gene Name

MMP14 recombinant protein

Product Synonym Names

Matrix metalloproteinase-14; MMP14; MMP-14; MMP-X1; MT-MMP; MT-MMP 1; MT1-MMP; MT1MMP; MTMMP1; WNCHRS

Sequence

ADLALASLGS AQSSSFSPEA WLQQYGYLPP GDLRTHTQRS PQSLSAAIAA MQKFYGLQVT GKADADTMKA MRRPRCGVPD KFGAEIKANV RRKRYAIQGL KWQHNEITFC IQNYTPKVGE YATYEAIRKA FRVWESATPL RFREVPYAYI REGHEKQADI MIFFAEGFHG DSTPFDGEGG FLAHAYFPGP NIGGDTHFDS AEPWTVRNED LNGNDIFLVA VHELGHALGL EHSSDPSAIM APFYQWMDTE NFVLPDDDRR GIQQLYGGES GFPTKMPPQP RTTSRPSVPD KPKNPTYGPN ICDGNFDTVA MLRGEMFVFK ERWFWRVRNN QVMDGYPMPI GQFWRGLPAS INTAYERKDG KFVFFKGDKH WVFDEASLEP GYPKHIKELG RGLPTDKIDA ALFWMPNGKT YFFRGNKYYR FNEELRAVDS EYPKNIKVWE GIPESPRGSF MGSDEVFTYF YKGNKYWKFN NQKLKVEPGY PKSALRDWMG CPSGGRPDEG TEEETEVIII EVDEEGGGAV SHHHHHH

Host

Baculovirus

Form/Format

Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.

Purity/Purification

> 90% by SDS-PAGE

Concentration

0.25mg/ml (determined by Absorbance at 280nm) (lot specific)

Preparation and Storage

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

59.9kDa (527aa) 35-70kDa (SDS-PAGE under reducing conditions)

OMIM

D26512 mRNA

3D Structure

ModBase 3D Structure for P50281

Antigen Species

Human

Tag Information

His-tag

Endotoxin Level

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Product Categories/Family for MMP14 recombinant protein

Cardiovascular; Angiogenesis; Adhesion & Movement

Applications Tested/Suitable for MMP14 recombinant protein

SDS-PAGE

Product References and Citations for MMP14 recombinant protein

Arndt A., et al. (2015) Biomed Res Int. 2015:185404.; Seiki M., et al. (2003) Cancer Lett. 194:1-11.

Other Notes

Small volumes of MMP14 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MMP14 purchase

MBS206467 is a recombinant protein targeting MMP14. Also known as MMP14, 21-538aa, Human, His-tag, Baculovirus. Host: Baculovirus.

Additional Details

NCBI/Uniprot data below describe general gene information for MMP14. It may not necessarily be applicable to this product.

NCBI GI #

4826834

NCBI GeneID

4323

NCBI Accession #

NP_004986

UniProt Primary Accession #

P50281

UniProt Secondary Accession #

Q6GSF3; Q92678; A8K5L0

UniProt Related Accession #

P50281

GenBank Nucleotide #

NM_004995.3

NCBI Official Full Name

matrix metalloproteinase-14 preproprotein

NCBI Official Synonym Full Names

matrix metallopeptidase 14

NCBI Official Symbol

MMP14

NCBI Official Synonym Symbols

MMP-14; MMP-X1; MT-MMP; MT1MMP; MTMMP1; WNCHRS; MT1-MMP; MT-MMP 1

UniProt Protein Name

Matrix metalloproteinase-14

UniProt Synonym Protein Names

MMP-X1; Membrane-type matrix metalloproteinase 1; MT-MMP 1; MTMMP1; Membrane-type-1 matrix metalloproteinase; MT1-MMP; MT1MMP

Protein Family

Matrix metalloproteinase

UniProt Gene Name

MMP14

UniProt Synonym Gene Names

MMP-14; MT-MMP 1; MTMMP1; MT1-MMP; MT1MMP

NCBI Protein Information

matrix metalloproteinase-14

NCBI Summary for MMP14

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion. [provided by RefSeq, Jul 2008]

UniProt Comments for MMP14

Endopeptidase that degrades various components of the extracellular matrix such as collagen. Activates progelatinase A. Essential for pericellular collagenolysis and modeling of skeletal and extraskeletal connective tissues during development (). May be involved in actin cytoskeleton reorganization by cleaving PTK7 (PubMed:20837484). Acts as a positive regulator of cell growth and migration via activation of MMP15. Involved in the formation of the fibrovascular tissues in association with pro-MMP2 (PubMed:12714657). Cleaves ADGRB1 to release vasculostatin-40 which inhibits angiogenesis (PubMed:22330140).

Research Articles on MMP14

1. In turn, LIMK1 and LIMK2 are required for MT1-MMP-dependent matrix degradation and cell invasion in a three-dimensional type I collagen environment.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with MMP14 recombinant protein

Reference Product	PubMed Publications
MMP2 recombinant protein	>201 publications with MMP14 and MMP2
MMP13 recombinant protein	>67 publications with MMP14 and MMP13
TIMP2 recombinant protein	>62 publications with MMP14 and TIMP2
TIMP1 recombinant protein	>45 publications with MMP14 and TIMP1
FURIN recombinant protein	>15 publications with MMP14 and FURIN
SP1 recombinant protein	>7 publications with MMP14 and SP1
DCN recombinant protein	>4 publications with MMP14 and DCN
NID1 recombinant protein	>1 publications with MMP14 and NID1

Pathways associated with MMP14 recombinant protein

Products by Pathway	Pathway Diagram
AGE/RAGE Pathway antibodies	AGE/RAGE Pathway Diagram
Activation Of Matrix Metalloproteinases Pathway antibodies	Activation Of Matrix Metalloproteinases Pathway Diagram
Collagen Degradation Pathway antibodies	Collagen Degradation Pathway Diagram
Degradation Of The Extracellular Matrix Pathway antibodies	Degradation Of The Extracellular Matrix Pathway Diagram
Extracellular Matrix Organization Pathway antibodies	Extracellular Matrix Organization Pathway Diagram
GnRH Signaling Pathway antibodies	GnRH Signaling Pathway Diagram
GnRH Signaling Pathway antibodies	GnRH Signaling Pathway Diagram
Gonadotropin-releasing Hormone (GnRH) Signaling Pathway antibodies	Gonadotropin-releasing Hormone (GnRH) Signaling Pathway Diagram
HIF-2-alpha Transcription Factor Network Pathway antibodies	HIF-2-alpha Transcription Factor Network Pathway Diagram
Matrix Metalloproteinases Pathway antibodies	Matrix Metalloproteinases Pathway Diagram

Diseases associated with MMP14 recombinant protein

Disease Name	Pubmed Publications
Neoplasm Invasiveness Antibodies	>175 publications with MMP14 and Neoplasm Invasiveness
Neoplasm Metastasis Antibodies	>137 publications with MMP14 and Neoplasm Metastasis
Breast Neoplasms Antibodies	>93 publications with MMP14 and Breast Neoplasms
Inflammation Antibodies	>61 publications with MMP14 and Inflammation
Adenocarcinoma Antibodies	>55 publications with MMP14 and Adenocarcinoma
Lung Diseases Antibodies	>47 publications with MMP14 and Lung Diseases
Neoplasms, Experimental Antibodies	>42 publications with MMP14 and Neoplasms, Experimental
Fibrosis Antibodies	>41 publications with MMP14 and Fibrosis
Lung Neoplasms Antibodies	>39 publications with MMP14 and Lung Neoplasms
Liver Neoplasms Antibodies	>26 publications with MMP14 and Liver Neoplasms

Organs/Tissues associated with MMP14 recombinant protein

Organ/Tissue Name	Pubmed Publications
Vascular Antibodies	>146 publications with MMP14 and Vascular
Blood Antibodies	>115 publications with MMP14 and Blood
Bone Antibodies	>86 publications with MMP14 and Bone
Lung Antibodies	>83 publications with MMP14 and Lung
Muscle Antibodies	>61 publications with MMP14 and Muscle
Connective Tissue Antibodies	>49 publications with MMP14 and Connective Tissue
Brain Antibodies	>49 publications with MMP14 and Brain
Embryonic Tissue Antibodies	>46 publications with MMP14 and Embryonic Tissue
Heart Antibodies	>37 publications with MMP14 and Heart
Skin Antibodies	>34 publications with MMP14 and Skin