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CES1D Baculovirus Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS206474

Ces1d, also known as carboxylesterase 1D, is a member of a large family of carboxylesterases that are responsible for the hydrolysis of ester and amide bonds. It is the principle lipase of white adipose tissue fat cake extracts. Partially purified white… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Ces1d, Active Protein
Full Product Name
Ces1d, 19-565aa, Mouse, His-tag, Baculovirus (Bioactivity Validated)
Product Gene Name
Ces1d active protein
Product Synonym Names
Carboxylesterase 1D; Ces1d; Ces3; TGH
Sequence
YPSSPPVVNT VKGKVLGKYV NLEGFTQPVA VFLGVPFAKP PLGSLRFAPP QPAEPWSFVK NTTSYPPMCS QDAVGGQVLS ELFTNRKENI PLQFSEDCLY LNIYTPADLT KNSRLPVMVW IHGGGLVVGG ASTYDGLALS AHENVVVVTI QYRLGIWGFF STGDEHSRGN WGHLDQVAAL RWVQDNIANF GGNPGSVTIF GESAGGFSVS VLVLSPLAKN LFHRAISESG VSLTAALITT DVKPIAGLVA TLSGCKTTTS AVMVHCLRQK TEDELLETSL KLNLFKLDLL GNPKESYPFL PTVIDGVVLP KAPEEILAEK SFSTVPYIVG INKQEFGWII PTLMGYPLAE GKLDQKTANS LLWKSYPTLK ISENMIPVVA EKYLGGTDDL TKKKDLFQDL MADVVFGVPS VIVSRSHRDA GASTYMYEFE YRPSFVSAMR PKAVIGDHGD EIFSVFGSPF LKDGASEEET NLSKMVMKFW ANFARNGNPN GGGLPHWPEY DQKEGYLKIG ASTQAAQRLK DKEVSFWAEL RAKESAQRPS HREHVELLEH HHHHH
Host
Baculovirus
Form/Format
Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
Purity/Purification
> 90% by SDS-PAGE
Concentration
0.5 mg/ml (determined by Absorbance at 280nm) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
60.9kDa (555aa) 50-70KDa (SDS-PAGE under reducing conditions.)
3D Structure
ModBase 3D Structure for Q8VCT4
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Antigen Species
Mouse
Tag Information
His-tag
Endotoxin Level
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Biological Activity
Specific activity is > 80,000 pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1pmole of p-nitrophenyl acetate to p-nitrophenol per minute at pH 7.5 at 37C

Product Categories/Family for Ces1d active protein

Signal Transduction; Metabolism; Enzymes & Proteases
Applications Tested/Suitable for Ces1d active protein
SDS-PAGE

SDS-Page of Ces1d active protein

Baculovirus CES1D Active Protein – Cat# MBS206474

Product References and Citations for Ces1d active protein

Soni KG., et al. (2004) J. Biol. Chem. 279:40683-40689.; Sanghani SP., et al. (2004) Drug Metab Dispos. 32:505-511.

Other Notes

Small volumes of Ces1d active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Ces1d purchase

MBS206474 is an active protein targeting Ces1d. Also known as Ces1d, 19-565aa, Mouse, His-tag, Baculovirus (Bioactivity Validated). Host: Baculovirus.

Additional Details

NCBI/Uniprot data below describe general gene information for Ces1d. It may not necessarily be applicable to this product.

NCBI GI #
117553604
NCBI GeneID
104158
NCBI Accession #
NP_444430
UniProt Primary Accession #
Q8VCT4
UniProt Secondary Accession #
Q91ZV9; Q924V8; Q9ULY1
UniProt Related Accession #
Q8VCT4
GenBank Nucleotide #
NM_053200.2
NCBI Official Full Name
carboxylesterase 1D
NCBI Official Synonym Full Names
carboxylesterase 1D
NCBI Official Symbol
Ces1d
NCBI Official Synonym Symbols
TGH; Ces3
UniProt Protein Name
Carboxylesterase 1D
UniProt Synonym Protein Names
Carboxylesterase 3 (EC:3.1.1.1, EC:3.1.1.67); Fatty acid ethyl ester synthase; FAEE synthase; Triacylglycerol hydrolase; TGH
Protein Family
Carboxylesterase
UniProt Gene Name
Ces1d
UniProt Synonym Gene Names
; FAEE synthase; TGH
NCBI Protein Information
carboxylesterase 1D

UniProt Comments for Ces1d

Major lipase in white adipose tissue. Involved in the metabolism of xenobiotics and of natural substrates. Hydrolyzes triacylglycerols and monoacylglycerols, with a preference for monoacylglycerols. The susceptibility of the substrate increases with decreasing acyl chain length of the fatty acid moiety. Catalyzes the synthesis of fatty acid ethyl esters.
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Research Articles on Ces1d

1. the carboxylesterase 1d (Ces1d) and carboxylesterase 1e (Ces1e) expression and the capacity of hydrolytic activity of liver and intestine decreased, whereas the Akt/mTOR/HIF-1alpha/ Stra13 (DEC1) signaling was activated in type 2 diabetes mice.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with Ces1d active protein

 Products by Pathway  Pathway Diagram
 Drug Metabolism - Other Enzymes Pathway antibodies  Drug Metabolism - Other Enzymes Pathway Diagram
 Drug Metabolism - Other Enzymes Pathway antibodies  Drug Metabolism - Other Enzymes Pathway Diagram
 Irinotecan Pathway antibodies  Irinotecan Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram

Diseases associated with Ces1d active protein

 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >2 publications with Ces1d and Disease Models, Animal
 Body Weight Changes Antibodies  >1 publications with Ces1d and Body Weight Changes
 Weight Gain Antibodies  >1 publications with Ces1d and Weight Gain

Organs/Tissues associated with Ces1d active protein

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >7 publications with Ces1d and Liver
 Intestine Antibodies  >3 publications with Ces1d and Intestine
 Muscle Antibodies  >1 publications with Ces1d and Muscle
 Skin Antibodies  >1 publications with Ces1d and Skin
 Lung Antibodies  >1 publications with Ces1d and Lung
 Kidney Antibodies  >1 publications with Ces1d and Kidney
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