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NT5E Baculovirus Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS206515

Nt5e, also known as 5-nucleotidase, is the main enzyme responsible for conversion of AMP into the immunosuppressive molecule adenosine. It was demonstrated to play a direct role in tumor progression including regulation of tumor vascularization. It is of… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Nt5e, Active Protein
Full Product Name
Nt5e, 29-551aa, Mouse,His-tag, Baculovirus (Bioactivity Validated)
Product Gene Name
Product Synonym Names
5-nucleotidase; Nt5e; 2210401F01Rik; AI447961; CD73; eNT; NT; Nt5; 5-NT
Sequence
WELTILHTND VHSRLEQTSD DSTKCLNASL CVGGVARLFT KVQQIRKEEP NVLFLDAGDQ YQGTIWFTVY KGLEVAHFMN ILGYDAMALG NHEFDNGVEG LIDPLLRNVK FPILSANIKA RGPLAHQISG LFLPSKVLSV GGEVVGIVGY TSKETPFLSN PGTNLVFEDE ISALQPEVDK LKTLNVNKII ALGHSGFEMD KLIAQKVRGV DIVVGGHSNT FLYTGNPPSK EVPAGKYPFI VTADDGRQVP VVQAYAFGKY LGYLKVEFDD KGNVITSYGN PILLNSSIPE DATIKADINQ WRIKLDNYST QELGRTIVYL DGSTQTCRFR ECNMGNLICD AMINNNLRHP DEMFWNHVSM CIVNGGGIRS PIDEKNNGTI TWENLAAVLP FGGTFDLVQL KGSTLKKAFE HSVHRYGQST GEFLQVGGIH VVYDINRKPW NRVVQLEVLC TKCRVPIYEP LEMDKVYKVT LPSYLANGGD GFQMIKDELL KHDSGDQDIS VVSEYISKMK VVYPAVEGRI KFSLEHHHHH H
Host
Baculovirus
Form/Format
Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
Purity/Purification
> 90% by SDS-PAGE
Concentration
0.5 mg/ml (determined by Absorbance at 280nm) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
59.1kDa (531aa) 50-70kDa (SDS-PAGE under reducing conditions)
3D Structure
ModBase 3D Structure for Q61503
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Antigen Species
Human
Tag Information
His-tag
Endotoxin Level
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Biological Activity
Specific activity is > 8,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of Adenosine 5-monophosphate to phosphate per minute per minute at pH 7.5 at 25C.

Product Categories/Family for Nt5e active protein

Applications Tested/Suitable for Nt5e active protein
SDS-PAGE

Product References and Citations for Nt5e active protein

Koszalka P., et al. (2016) PLoS One. 11:e0151420.; Braun N., et al. (1995) Brain Res Dev Brain Res. 88:79-86.

Other Notes

Small volumes of Nt5e active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Nt5e purchase

MBS206515 is an active protein targeting Nt5e. Also known as Nt5e, 29-551aa, Mouse,His-tag, Baculovirus (Bioactivity Validated). Host: Baculovirus.

Additional Details

NCBI/Uniprot data below describe general gene information for Nt5e. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
5'-nucleotidase preproprotein
NCBI Official Synonym Full Names
5' nucleotidase, ecto
NCBI Official Symbol
Nt5e
NCBI Official Synonym Symbols
NT; Nt5; eNT; CD73; 5'-NT; AI447961; 2210401F01Rik
UniProt Protein Name
5'-nucleotidase
UniProt Synonym Protein Names
Ecto-5'-nucleotidase; CD_antigen: CD73
UniProt Gene Name
Nt5e
UniProt Synonym Gene Names
Nt5; Nte; 5'-NT
NCBI Protein Information
5'-nucleotidase

NCBI Summary for Nt5e

This gene encodes a membrane-bound nucleotidase that hydrolyzes extracellular nucleoside monophosphates. The encoded preproprotein undergoes proteolytic processing to generate to a functional, homodimeric enzyme that preferentially uses adenosine monophosphate as a substrate to generate free adenosine. Mice lacking the encoded protein exhibit a significantly reduced fall in stop flow pressure and superficial nephron glomerular filtration rate in response to a saturating increase of tubular perfusion flow. [provided by RefSeq, Sep 2016]
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UniProt Comments for Nt5e

Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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