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PHPT1 Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS206566

PHPT1 is a 125 amino acid enzyme belonging to the Janus protein family. Existing as a monomer in the cytoplasm, PHPT1 is an EDTA-insensitive phosphohistidine phosphatase. Overexpression of PHPT1 leads to specific phosphohistidine phosphatase activity towards… More details

$180 / 0.004 mg +1 FREE 8GB USB
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PHPT1, Active Protein
Full Product Name
PHPT1, 1-125aa, Human, His-tag, E Coli (Bioactivity Validated)
Product Gene Name
Product Synonym Names
14 kDa phosphohistidine phosphatas; bA216L13.10; CGI-202; DKFZp564M173; HSPC141; PHP14; RP11-216L13.10
Sequence
MGSSHHHHHH SSGLVPRGSH MAVADLALIP DVDIDSDGVF KYVLIRVHSA PRSGAPAAES KEIVRGYKWA EYHADIYDKV SGDMQKQGCD CECLGGGRIS HQSQDKKIHV YGYSMAYGPA QHAISTEKIK AKYPDYEVTW ANDGY
Host
E Coli
Form/Format
Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 0.2M NaCl, 2mM DTT, 10% glycerol
Purity/Purification
> 95% by SDS-PAGE
Concentration
0.5 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
15.9 kDa (145aa), confirmed by MALDI-TOF
OMIM
AF393504 mRNA
3D Structure
ModBase 3D Structure for Q9NRX4
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Antigen Species
Human
Tag Information
His-tag
Biological Activity
Specific activity is >120 units/mg, and is defined as the amount of enzyme that hydrolyze 1nmol f p-nitrophenyl phosphate per minute at pH7.5 at 37C.

Product Categories/Family for PHPT1 active protein

Applications Tested/Suitable for PHPT1 active protein
SDS-PAGE

Product References and Citations for PHPT1 active protein

Srivastava S., et al. (2008) Proc Natl Acad Sci U S A. 105 (38):14442-6.; van Vlierberghe P., et al. (2006) Leukemia. 20 (7):1245-53.

Other Notes

Small volumes of PHPT1 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PHPT1 purchase

MBS206566 is an active protein targeting PHPT1. Also known as PHPT1, 1-125aa, Human, His-tag, E Coli (Bioactivity Validated). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for PHPT1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
14 kDa phosphohistidine phosphatase isoform 3
NCBI Official Synonym Full Names
phosphohistidine phosphatase 1
NCBI Official Symbol
PHPT1
NCBI Official Synonym Symbols
PHP; PHP14; CGI-202; HSPC141; HEL-S-132P
UniProt Protein Name
14 kDa phosphohistidine phosphatase
UniProt Synonym Protein Names
Phosphohistidine phosphatase 1
UniProt Gene Name
PHPT1
UniProt Synonym Gene Names
PHP14; PHP
NCBI Protein Information
14 kDa phosphohistidine phosphatase

NCBI Summary for PHPT1

This gene encodes an enzyme that catalyzes the reversible dephosphorylation of histidine residues in proteins. It may be involved in the dephosphorylation of G-beta and ATP citrate lyase and in negatively regulating CD4 T lymphocytes by dephosphorylation and inhibition of KCa3.1 channels. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]
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UniProt Comments for PHPT1

Exhibits phosphohistidine phosphatase activity.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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