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CHI3L2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS206588

CHI3L2, also known as chitinase-3-like protein 2, belongs to chitinase protein family. It is secreted by synovial fibroblasts, chondrocytes, and TGFb/IL4 stimulated monocyte derived macrophages. It has been proposed as a potential biomarker for osteoarthritis. More details

$285 / 0.05 mL +1 FREE 8GB USB
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
CHI3L2, Monoclonal Antibody
Full Product Name
CHI3L2 antibody
Product Gene Name
Product Synonym Names
CHI3L2; CHIL2; YKL-39; YKL39
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
AT2B5
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-A affinity chromatography
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
OMIM
U58515 mRNA
3D Structure
ModBase 3D Structure for Q15782
Immunogen
Recombinant human CHI3L2 (27-390aa) purified from E. coli

Product Categories/Family for anti-CHI3L2 antibody

Applications Tested/Suitable for anti-CHI3L2 antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC)
Application Notes for anti-CHI3L2 antibody
Usage: The antibody has been tested by ELISA, Western Blot, and Flow Cytometry analysis to assure specificity and reactivity. Since application varies , however, each investigation should be titrated by the reagent to obtain optimal results.

Western Blot (WB) of anti-CHI3L2 antibody

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CHI3L2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: HeLa cell lysate Lane 3.: HepG2 cell lysate Lane 4.: LnCap cell lysate
Mouse Anti-CHI3L2 Monoclonal Antibody – Cat# MBS206588

Flow Cytometry (FC) of anti-CHI3L2 antibody

Flow cytometry analysis of CHI3L2 in HeLa cells. The cell was stained with MBS206588 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray)
Mouse Anti-CHI3L2 Monoclonal Antibody, Flow Cytometry (FC) – Cat# MBS206588

Product References and Citations for anti-CHI3L2 antibody

Hu B., et al. (1996) J. Biol. Chem. 271: 19415-19420.; Gratchev A., et al. (2008) Biomarker Insights. 3: 39-44.

Other Notes

Small volumes of anti-CHI3L2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CHI3L2 purchase

MBS206588 is an antibody targeting CHI3L2. Also known as CHI3L2 antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CHI3L2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
chitinase-3-like protein 2 isoform a
NCBI Official Synonym Full Names
chitinase 3 like 2
NCBI Official Symbol
CHI3L2
NCBI Official Synonym Symbols
CHIL2; YKL39; YKL-39
UniProt Protein Name
Chitinase-3-like protein 2
UniProt Synonym Protein Names
Chondrocyte protein 39; YKL-39
UniProt Gene Name
CHI3L2
NCBI Protein Information
chitinase-3-like protein 2

NCBI Summary for CHI3L2

The protein encoded by this gene is similar to bacterial chitinases but lacks chitinase activity. The encoded protein is secreted and is involved in cartilage biogenesis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

UniProt Comments for CHI3L2

Lectin that binds chitooligosaccharides and other glycans with high affinity, but not heparin. Has no chitinase activity.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CHI3L2 antibody

 Reference Product  PubMed Publications
 CHID1 antibody  >3 publications with CHI3L2 and CHID1
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