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Calnexin (CANX) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS206599

Calnexin, also known as IP90, p88 and p90, is a member of the calnexin family of molecular chaperones. This protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins,… More details

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Product Overview

Online Datasheet in PDF
Product Name
Calnexin (CANX), Monoclonal Antibody
Full Product Name
Calnexin antibody
Product Gene Name
Product Synonym Names
CNX; IP90; P90; CANX; FLJ26570; Histocompatibility complex class I antigen binding protein p88
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
AT18B9
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-A affinity chromatography
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C to 8 degree C.
For long term storage, aliquot and store at -20 degree C to -80 degree C.
Avoid repeated freezing and thawing cycles.
OMIM
L10284 mRNA
3D Structure
ModBase 3D Structure for P27824
Immunogen
Recombinant human Calnexin (21-481aa) purified from E. coli.
Applications Tested/Suitable for anti-CANX antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-CANX antibody
Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Western Blot (WB) of anti-CANX antibody

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Calnexin antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: MCF7 cell lysate Lane 3.: A431 cell lysate Lane 4.: A549 cell lysate Lane 5.: 293T cell lysate Lane 6.: HepG2 cell lysate Lane 7.: Jurkat cell lysate
Mouse Anti-CANX Monoclonal Antibody – Cat# MBS206599

Immunofluorescence (IF) of anti-CANX antibody

ICC/IF analysis of Calnexin in HeLa cells. The cell was stained with MBS206599 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Mouse Anti-CANX Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS206599

Product References and Citations for anti-CANX antibody

Wang B., et al. (2009) Immunology. 128 (1): 43-57.; Millar DJ., et al. (2009) Proteomics. 9 (9): 2355-72.

Other Notes

Small volumes of anti-CANX antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CANX purchase

MBS206599 is an antibody targeting CANX. Also known as Calnexin antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CANX. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
calnexin
NCBI Official Synonym Full Names
calnexin
NCBI Official Symbol
CANX
NCBI Official Synonym Symbols
CNX; P90; IP90
UniProt Protein Name
Calnexin
UniProt Synonym Protein Names
IP90; Major histocompatibility complex class I antigen-binding protein p88; p90
Protein Family
UniProt Gene Name
CANX
NCBI Protein Information
calnexin

NCBI Summary for CANX

This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008]
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UniProt Comments for CANX

Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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