Luciferase Active Protein

Name: Active Protein; Luciferase; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS206631
Price: 215 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS206631

LUC, also known as luciferase. LUC catalyzes the oxidative decarboxylation of coelenterazine in the presence of dissolvedoxygen to yield oxyluciferin, CO2, and blue light. In vivo, the excited state luciferin-luciferase complex undergoes the process of… More details

$215 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Luciferase, Active Protein

Full Product Name

Luciferase, 1-311aa, Renilla reniformis, His-tag, E Coli (Bioactivity Validated)

Product Synonym Names

Luciferase

Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMTSKVY DPEQRKRMIT GPQWWARCKQ MNVLDSFINY YDSEKHAENA VIFLHGNAAS SYLWRHVVPH IEPVARCIIP DLIGMGKSGK SGNGSYRLLD HYKYLTAWFE LLNLPKKIIF VGHDWGACLA FHYSYEHQDK IKAIVHAESV VDVIESWDEW PDIEEDIALI KSEEGEKMVL ENNFFVETML PSKIMRKLEP EEFAAYLEPF KEKGEVRRPT LSWPREIPLV KGGKPDVVQI VRNYNAYLRA SDDLPKMFIE SDPGFFSNAI VEGAKKFPNT EFVKVKGLHF SQEDAPDEMG KYIKSFVERV LKNEQ

Host

E Coli

Form/Format

Liquid. 20mM Tris-HCl (pH 8.0) containing 1mM DTT, 10% glycerol

Purity/Purification

> 95% by SDS-PAGE

Concentration

0.5 mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

38.5 kDa (335aa) Confirmed by MALDI-TOF

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Antigen Species

Renilla reniformis

Tag Information

His-tag

Biological Activity

Specific activity is >1X109 light units/mg. One luciferase enzyme units will produce one Relative Light Unit (RLU) at pH7.5 at 25C.

Product Categories/Family for Luciferase active protein

Enzymes & Proteases

Applications Tested/Suitable for Luciferase active protein

SDS-PAGE

Product References and Citations for Luciferase active protein

Lorenz WW., et al. (1991) Proc Natl Acad Sci U S A. 88 (10):4438-42.

Other Notes

Small volumes of Luciferase active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Luciferase purchase

MBS206631 is an active protein targeting Luciferase. Also known as Luciferase, 1-311aa, Renilla reniformis, His-tag, E Coli (Bioactivity Validated). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for Luciferase. It may not necessarily be applicable to this product.

NCBI GI #

160820

NCBI Accession #

AAA29804

NCBI Official Full Name

luciferase

UniProt Protein Name

Coelenterazine h 2-monooxygenase

UniProt Synonym Protein Names

Renilla-luciferin 2-monooxygenase; Renilla-type luciferase

Protein Family

Luciferin 4-monooxygenase

UniProt Comments for Luciferase

Upon binding the substrate, the enzyme catalyzes an oxygenation, producing a very short-lived hydroperoxide that cyclizes into a dioxetanone structure, which collapses, releasing a CO2 molecule. The spontaneous breakdown of the dioxetanone releases the energy (about 50 kcal/mole) that is necessary to generate the excited state of the coelenteramide product, which is the singlet form of the monoanion. In vivo the product undergoes the process of nonradiative energy transfer to an accessory protein, a green fluorescent protein (GFP), which results in green bioluminescence. In vitro, in the absence of GFP, the product emits blue light.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.