Baculovirus GZMB Active Protein – Cat# MBS206823

GZMB Baculovirus Active Protein

Name: GZMB Active Protein; Baculovirus; MyBioSource
Brand: MyBioSource
SKU: MBS206823
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS206823

GZMB, also known as granzyme B isoform 1, is member of the granzyme subfamily of proteins, part of the peptidase S1 family of serine proteases. Tis protein is secreted by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) and proteolytically… More details

$295 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

GZMB, Active Protein

Full Product Name

GZMB, 19-247aa, Human, His-tag, Baculovirus (Bioactivity Validated)

Product Gene Name

GZMB active protein

Product Synonym Names

Granzyme B isoform 1; GZMB; C11; CCPI; CGL-1; CGL1; CSP-B; CSPB; CTLA1; CTSGL1; HLP; SECT

Sequence

GEIIGGHEAK PHSRPYMAYL MIWDQKSLKR CGGFLIRDDF VLTAAHCWGS SINVTLGAHN IKEQEPTQQF IPVKRPIPHP AYNPKNFSND IMLLQLERKA KRTRAVQPLR LPSNKAQVKP GQTCSVAGWG QTAPLGKHSH TLQEVKMTVQ EDRKCESDLR HYYDSTIELC VGDPEIKKTS FKGDSGGPLV CNKVAQGIVS YGRNNGMPPR ACTKVSSFVH WIKKTMKRYH HHHHH

Host

Baculovirus

Form/Format

Liquid. In phosphate buffered saline (pH 7.4) containing 20% glycerol, 1mM DTT.

Purity/Purification

> 95% by SDS-PAGE

Concentration

0.5 mg/ml (determined by Absorbance at 280nm) (lot specific)

Preparation and Storage

Can be stored at 4°C short term (1 week).
For long term storage, aliquot and store at -20°C or -80°C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

26.5kDa (235aa)

OMIM

M17016 mRNA

3D Structure

ModBase 3D Structure for P10144

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Tag Information

His-tag

Domain

19-247AA

Endotoxin Level

< 1.0 EU per 1ug of protein (determined by LAL method)

Biological Activity

Specific activity is > 7,000 pmol/min/ug, and is defined as the amount of enzyme that cleave 1pmole of Boc-Ala-Ala-Asp-SBzl at 37C.

Dry Ice Shipment

Extra charge fee may add to your shipping cost as dry ice is required to ship this product.

Product Categories/Family for GZMB active protein

Apoptosis; Enzymes & Proteases

Applications Tested/Suitable for GZMB active protein

SDS-PAGE

Product References and Citations for GZMB active protein

Poe M., et al, (1991) J. Biol. Chem. 266:98-103. Xu W., et al, (2014) Eur. J. Immunol. 44 (1) 275-284.

Other Notes

Small volumes of GZMB active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GZMB purchase

MBS206823 is an active protein targeting GZMB. Also known as GZMB, 19-247aa, Human, His-tag, Baculovirus (Bioactivity Validated). Host: Baculovirus.

Additional Details

NCBI/Uniprot data below describe general gene information for GZMB. It may not necessarily be applicable to this product.

NCBI GI #

221625528

NCBI GeneID

3002

NCBI Accession #

NP_004122.2

UniProt Primary Accession #

P10144

UniProt Secondary Accession #

Q8N1D2; Q9UCC1

UniProt Related Accession #

P10144

GenBank Nucleotide #

NM_004131.5

NCBI Official Full Name

granzyme B isoform 1 preproprotein

NCBI Official Synonym Full Names

granzyme B

NCBI Official Symbol

GZMB

NCBI Official Synonym Symbols

C11; HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1

UniProt Protein Name

Granzyme B

UniProt Synonym Protein Names

C11; CTLA-1; Cathepsin G-like 1; CTSGL1; Cytotoxic T-lymphocyte proteinase 2; Lymphocyte protease; Fragmentin-2; Granzyme-2; Human lymphocyte protein; HLP; SECT; T-cell serine protease 1-3E

Protein Family

Granzyme

UniProt Gene Name

GZMB

UniProt Synonym Gene Names

CGL1; CSPB; CTLA1; GRB; CTSGL1; Lymphocyte protease; HLP

NCBI Protein Information

granzyme B

NCBI Summary for GZMB

This gene encodes a member of the granzyme subfamily of proteins, part of the peptidase S1 family of serine proteases. The encoded preproprotein is secreted by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) and proteolytically processed to generate the active protease, which induces target cell apoptosis. This protein also processes cytokines and degrades extracellular matrix proteins, and these roles are implicated in chronic inflammation and wound healing. Expression of this gene may be elevated in human patients with cardiac fibrosis. [provided by RefSeq, Sep 2016]

UniProt Comments for GZMB

This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.

Research Articles on GZMB

1. Granzyme B breaches the inner membrane through Tim22, the metabolite carrier translocase pore, in a mitochondrial heat-shock protein 70-dependent manner.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GZMB active protein

Reference Product	PubMed Publications
CASP3 active protein	>93 publications with GZMB and CASP3
BID active protein	>46 publications with GZMB and BID
PRF1 active protein	>40 publications with GZMB and PRF1
CASP8 active protein	>32 publications with GZMB and CASP8
CASP7 active protein	>13 publications with GZMB and CASP7
IL2RA active protein	>10 publications with GZMB and IL2RA
IL2 active protein	>4 publications with GZMB and IL2
CASP10 active protein	>2 publications with GZMB and CASP10
IL2RB active protein	>1 publications with GZMB and IL2RB

Pathways associated with GZMB active protein

Products by Pathway	Pathway Diagram
Activation, Myristolyation Of BID And Translocation To Mitochondria Pathway antibodies	Activation, Myristolyation Of BID And Translocation To Mitochondria Pathway Diagram
Allograft Rejection Pathway antibodies	Allograft Rejection Pathway Diagram
Allograft Rejection Pathway antibodies	Allograft Rejection Pathway Diagram
Allograft Rejection Pathway antibodies	Allograft Rejection Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Autoimmune Thyroid Disease Pathway antibodies	Autoimmune Thyroid Disease Pathway Diagram
Autoimmune Thyroid Disease Pathway antibodies	Autoimmune Thyroid Disease Pathway Diagram

Diseases associated with GZMB active protein

Disease Name	Pubmed Publications
Necrosis Antibodies	>160 publications with GZMB and Necrosis
Inflammation Antibodies	>98 publications with GZMB and Inflammation
Disease Models, Animal Antibodies	>70 publications with GZMB and Disease Models, Animal
Neoplasms, Experimental Antibodies	>48 publications with GZMB and Neoplasms, Experimental
Lung Diseases Antibodies	>48 publications with GZMB and Lung Diseases
Nervous System Diseases Antibodies	>47 publications with GZMB and Nervous System Diseases
Liver Diseases Antibodies	>40 publications with GZMB and Liver Diseases
Cardiovascular Diseases Antibodies	>34 publications with GZMB and Cardiovascular Diseases
Breast Neoplasms Antibodies	>25 publications with GZMB and Breast Neoplasms
Hepatitis Antibodies	>21 publications with GZMB and Hepatitis

Organs/Tissues associated with GZMB active protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>1039 publications with GZMB and Blood
Kidney Antibodies	>99 publications with GZMB and Kidney
Lung Antibodies	>93 publications with GZMB and Lung
Liver Antibodies	>88 publications with GZMB and Liver
Spleen Antibodies	>78 publications with GZMB and Spleen
Bone Marrow Antibodies	>59 publications with GZMB and Bone Marrow
Heart Antibodies	>44 publications with GZMB and Heart
Intestine Antibodies	>36 publications with GZMB and Intestine
Pancreas Antibodies	>18 publications with GZMB and Pancreas
Stomach Antibodies	>11 publications with GZMB and Stomach