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Aspartate Aminotransferase (AST) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2090411
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MBS2090411 is an antibody targeting AST. Also known as Aspartate Aminotransferase (AST) Monoclonal Antibody. Reactive in Mouse, Human, Rat. Host: Mouse. More details

$225 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Aspartate Aminotransferase (AST), Monoclonal Antibody
Full Product Name
Aspartate Aminotransferase (AST) Monoclonal Antibody
Product Gene Name
anti-AST antibody
Product Synonym Names
Monoclonal Antibody to Aspartate Aminotransferase (AST); cCAT; AAT; ASAT; SGOT; GOT1; Cysteine transaminase, cytoplasmic; Transaminase A; Aspartate Transaminase 1, Cytoplasmic; Glutamic-Oxaloacetic Transaminase 1, Soluble
Clonality
Monoclonal
Isotype
IgG2b Kappa
Clone Number
C5
Host
Mouse
Species Reactivity
Mouse, Human, Rat
Form/Format
Liquid
Purity/Purification
Protein A + Protein G affinity chromatography
Concentration
1mg/ml (lot specific)
Specificity
The antibody is a mouse monoclonal antibody raised against AST. It has been selected for its ability to recognize AST in immunohistochemical staining and western blotting.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Matching Pairs
Unconjugated Antibody: AST Clone #C5 (MBS2090411)
Immunogen: AST (MBS2010629)
Matching Pairs
Unconjugated Antibody: Aspartate Aminotransferase (AST) Clone #C5 (MBS2090411)
Biotin Conjugated Secondary Antibody: IgG (MBS2086030)
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Molecular Weight
46,248 Da
3D Structure
ModBase 3D Structure for P05201
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Organism Species
Mouse
Immunogen
Recombinant Aspartate Aminotransferase (AST)
Cross Reactivity
Mouse
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-AST antibody
Western Blot (WB), IHC, Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes for anti-AST antibody
Western Blot: 0.5-5ug/ml
Immunocytochemistry in formalin fixed cells: 5-30ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-30ug/ml
Immunohistochemistry in paraffin section: 5-30ug/ml
ELISA: 0.05-2ug/ml

Western Blot (WB) of anti-AST antibody

Western Blot: Sample: Recombinant AST, Mouse.
Mouse Anti-AST Monoclonal Antibody – Cat# MBS2090411

Other Notes

Small volumes of anti-AST antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for AST. It may not necessarily be applicable to this product.

NCBI GI #
160298209
NCBI GeneID
14718
NCBI Accession #
NP_034454.2
UniProt Primary Accession #
P05201
UniProt Secondary Accession #
Q3UJH8
UniProt Related Accession #
P05201
GenBank Nucleotide #
NM_010324.2
NCBI Official Full Name
aspartate aminotransferase, cytoplasmic
NCBI Official Synonym Full Names
glutamic-oxaloacetic transaminase 1, soluble
NCBI Official Symbol
Got1
NCBI Official Synonym Symbols
cCAT; Got-1; cAspAT; AI789014
UniProt Protein Name
Aspartate aminotransferase, cytoplasmic
UniProt Synonym Protein Names
Cysteine aminotransferase, cytoplasmic; Cysteine transaminase, cytoplasmic; cCAT; Glutamate oxaloacetate transaminase 1; Transaminase A
Protein Family
Allatostatin
UniProt Gene Name
Got1
UniProt Synonym Gene Names
cAspAT; cCAT
NCBI Protein Information
aspartate aminotransferase, cytoplasmic

UniProt Comments for AST

GOT1: Plays a key role in amino acid metabolism. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - cysteine and methionine; Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; Amino Acid Metabolism - tyrosine; EC 2.6.1.1; EC 2.6.1.3; Transferase

Chromosomal Location of Human Ortholog: 19 C3|19 36.67 cM

Cellular Component: axon terminus; cytoplasm; cytosol; extracellular exosome; lysosome; nucleoplasm; nucleus

Molecular Function: carboxylic acid binding; catalytic activity; L-aspartate:2-oxoglutarate aminotransferase activity; L-cysteine:2-oxoglutarate aminotransferase activity; phosphatidylserine decarboxylase activity; pyridoxal phosphate binding; transaminase activity; transferase activity

Biological Process: amino acid biosynthetic process; amino acid metabolic process; aspartate biosynthetic process; aspartate catabolic process; aspartate metabolic process; biosynthetic process; dicarboxylic acid metabolic process; fatty acid homeostasis; glutamate catabolic process to 2-oxoglutarate; glutamate catabolic process to aspartate; glycerol biosynthetic process; Notch signaling pathway; oxaloacetate metabolic process
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Research Articles on AST

1. cAspAT is a new member of glyceroneogenesis, transcriptionally regulated by TZD via the control of RORalpha expression by PPARgamma in adipocytes

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-AST antibody

 Products by Pathway  Pathway Diagram
 2-Oxocarboxylic Acid Metabolism Pathway antibodies  2-Oxocarboxylic Acid Metabolism Pathway Diagram
 2-Oxocarboxylic Acid Metabolism Pathway antibodies  2-Oxocarboxylic Acid Metabolism Pathway Diagram
 4-hydroxyproline Degradation I Pathway antibodies  4-hydroxyproline Degradation I Pathway Diagram
 Alanine And Aspartate Metabolism Pathway antibodies  Alanine And Aspartate Metabolism Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Amino Acid Metabolism Pathway antibodies  Amino Acid Metabolism Pathway Diagram
 Amino Acid Synthesis And Interconversion (transamination) Pathway antibodies  Amino Acid Synthesis And Interconversion (transamination) Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram

Diseases associated with anti-AST antibody

 Disease Name  Pubmed Publications
 Lung Neoplasms Antibodies  >6 publications with AST and Lung Neoplasms
 Disease Models, Animal Antibodies  >5 publications with AST and Disease Models, Animal
 Nervous System Diseases Antibodies  >5 publications with AST and Nervous System Diseases
 Liver Diseases Antibodies  >4 publications with AST and Liver Diseases
 Fatty Liver Antibodies  >3 publications with AST and Fatty Liver
 Brain Diseases Antibodies  >3 publications with AST and Brain Diseases
 Congenital Abnormalities Antibodies  >3 publications with AST and Congenital Abnormalities
 Cardiovascular Diseases Antibodies  >3 publications with AST and Cardiovascular Diseases
 Liver Neoplasms Antibodies  >2 publications with AST and Liver Neoplasms
 Neoplasm Metastasis Antibodies  >2 publications with AST and Neoplasm Metastasis

Organs/Tissues associated with anti-AST antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >17 publications with AST and Blood
 Liver Antibodies  >8 publications with AST and Liver
 Lung Antibodies  >7 publications with AST and Lung
 Brain Antibodies  >5 publications with AST and Brain
 Heart Antibodies  >4 publications with AST and Heart
 Intestine Antibodies  >4 publications with AST and Intestine
 Spinal Cord Antibodies  >3 publications with AST and Spinal Cord
 Muscle Antibodies  >3 publications with AST and Muscle
 Bone Antibodies  >2 publications with AST and Bone
 Prostate Antibodies  >1 publications with AST and Prostate
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