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CD32 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS210308

Mouse anti Human CD32 antibody, clone AT10 recognizes the human CD32 antigen, a 40kDa glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII). The antigen mediates several functions including endocytosis, activation of… More details

$510 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD32, Monoclonal Antibody
Full Product Name
MOUSE ANTI HUMAN CD32:Biotin
Product Gene Name
Clonality
Monoclonal
Isotype
IgG1
Clone Number
AT10
Host
Mouse
Form/Format
Biotin
Purified IgG conjugated to Biotin - liquid
Concentration
IgG concentration 0.1 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Molecular Weight
34,930 Da
OMIM
146790
Perservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
Preparation||Purified IgG prepared by affinity chromatography on Protein A
Immunogen
K562 cell line
Fusion Partners||Spleen cells from immunised BALB/c mice were fused with cells of the NS1 myeloma cell line.
Buffer Solution||Phosphate buffered saline
Target Species
Human
Applications Tested/Suitable for anti-CD32 antibody
Flow cytometry (FC/FACS)
Application Notes for anti-CD32 antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
Flow Cytometry: Maximum Dilution: Neat

Testing Data of anti-CD32 antibody

Staining of human peripheral blood monocytes with Mouse anti Human CD32: Biotin
Mouse Anti-CD32 Monoclonal Antibody – Cat# MBS210308

Testing Data of anti-CD32 antibody

Staining of human peripheral blood granulocytes with Mouse anti Human CD32:FITC (MBS210611)
Mouse Anti-CD32 Monoclonal Antibody, Testing Data – Cat# MBS210308

Testing Data of anti-CD32 antibody

Staining of human peripheral blood granulocytes with Mouse anti Human CD32:RPE (MBS213549)
Mouse Anti-CD32 Monoclonal Antibody, Testing Data – Cat# MBS210308

Testing Data of anti-CD32 antibody

Staining of human peripheral blood granulocytes with Mouse anti Human CD32 (MBS212021)
Mouse Anti-CD32 Monoclonal Antibody, Testing Data – Cat# MBS210308

Testing Data of anti-CD32 antibody

Staining of human peripheral blood granulocytes with Mouse anti Human CD32:Alexa Fluor 647 (MBS212021A647)
Mouse Anti-CD32 Monoclonal Antibody, Testing Data – Cat# MBS210308

Other Notes

Small volumes of anti-CD32 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD32 purchase

MBS210308 is an antibody targeting CD32. Also known as MOUSE ANTI HUMAN CD32:Biotin. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD32. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
low affinity immunoglobulin gamma Fc region receptor II-a isoform 1
NCBI Official Synonym Full Names
Fc fragment of IgG, low affinity IIa, receptor (CD32)
NCBI Official Symbol
FCGR2A
NCBI Official Synonym Symbols
CD32; FCG2; FcGR; CD32A; CDw32; FCGR2; IGFR2; FCGR2A1
UniProt Protein Name
Low affinity immunoglobulin gamma Fc region receptor II-a
UniProt Synonym Protein Names
CDw32; Fc-gamma RII-a; Fc-gamma-RIIa; FcRII-a; CD_antigen: CD32
Protein Family
UniProt Gene Name
FCGR2A
UniProt Synonym Gene Names
CD32; FCG2; FCGR2A1; IGFR2; IgG Fc receptor II-a; Fc-gamma-RIIa; FcRII-a
UniProt Entry Name
FCG2A_HUMAN
NCBI Protein Information
low affinity immunoglobulin gamma Fc region receptor II-a; Immunoglobulin G Fc receptor II; fc-gamma RII-a; fc-gamma-RIIa; fcRII-a; igG Fc receptor II-a

NCBI Summary for CD32

This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2008]
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UniProt Comments for CD32

FCGR2A: low affinity receptor for the Fc region of IgGs. Binding to IgG initiates cellular responses against pathogens and soluble antigens. A type I membrane protein found on monocytes, neutrophils and platelets. SHP-1 associates with this protein to modulate signaling events in myeloid cells.

Protein type: Cell surface; Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 1q23

Cellular Component: integral to membrane; plasma membrane

Molecular Function: IgG binding

Biological Process: innate immune response

Disease: Systemic Lupus Erythematosus; Malaria, Susceptibility To
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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