Mouse Anti-CD99 Monoclonal Antibody – Cat# MBS210788

CD99 Antibody – Mouse Monoclonal

Name: CD99 Antibody; Monoclonal; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS210788
Price: 545 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS210788

Mouse anti human CD99 antibody, clone DN16 recognizes human CD99, also known as E2 antigen, MIC2 or 12E7. CD99 is a 185 amino acid ~32kDa single pass type I transmembrane O-glycosylated glycoprotein. Three isoforms can be producted by alternative splicing.… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

CD99, Monoclonal Antibody

Full Product Name

MOUSE ANTI HUMAN CD99:FITC

Product Gene Name

anti-CD99 antibody

Clonality

Monoclonal

Isotype

IgG1

Clone Number

DN16

Host

Mouse

Form/Format

FITC
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Concentration

IgG concentration 0.1 mg/ml (lot specific)

Preparation and Storage

Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Molecular Weight

17,128 Da

OMIM

313470

3D Structure

ModBase 3D Structure for P14209

Perservative Stabilisers

0.09% Sodium Azide
1% Bovine Serum Albumin
Preparation||Purified IgG prepared by ion exchange chromatography

Buffer Solution

Phosphate buffered saline

Target Species

Human

Applications Tested/Suitable for anti-CD99 antibody

Flow cytometry (FC/FACS)

Application Notes for anti-CD99 antibody

Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Maximum Dilution: Neat

Other Notes

Small volumes of anti-CD99 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD99 purchase

MBS210788 is an antibody targeting CD99. Also known as MOUSE ANTI HUMAN CD99:FITC. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD99. It may not necessarily be applicable to this product.

NCBI GI #

171543879

NCBI GeneID

4267

NCBI Accession #

NP_001116370.1

UniProt Primary Accession #

P14209

UniProt Secondary Accession #

O00518; Q6ICV7; A6NIW1

UniProt Related Accession #

P14209

GenBank Nucleotide #

NM_001122898.1

NCBI Official Full Name

CD99 antigen isoform b

NCBI Official Synonym Full Names

CD99 molecule

NCBI Official Symbol

CD99

NCBI Official Synonym Symbols

MIC2; HBA71; MIC2X; MIC2Y; MSK5X

UniProt Protein Name

CD99 antigen

UniProt Synonym Protein Names

12E7; E2 antigen; Protein MIC2; T-cell surface glycoprotein E2; CD_antigen: CD99

Protein Family

CD99 antigen

UniProt Gene Name

CD99

UniProt Synonym Gene Names

MIC2; MIC2X; MIC2Y

UniProt Entry Name

CD99_HUMAN

NCBI Protein Information

CD99 antigen; E2 antigen; MIC2 (monoclonal antibody 12E7); T-cell surface glycoprotein E2; antigen identified by monoclonal 12E7, Y homolog; antigen identified by monoclonal antibodies 12E7, F21 and O13; surface antigen MIC2

NCBI Summary for CD99

The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of CD99. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2013]

UniProt Comments for CD99

CD99: Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes. Belongs to the CD99 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: Xp22.32 and Yp11.3

Cellular Component: focal adhesion; integral to plasma membrane; cytoplasm; plasma membrane

Biological Process: cell adhesion

Research Articles on CD99

1. dermatofibromastrongly expresses CD99 in a diffuse pattern that may serve as evidence in distinction from dermatofibrosarcoma

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD99 antibody

Reference Product	PubMed Publications
EWSR1 antibody	>112 publications with CD99 and EWSR1

Pathways associated with anti-CD99 antibody

Products by Pathway	Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Leukocyte Transendothelial Migration Pathway antibodies	Leukocyte Transendothelial Migration Pathway Diagram
Leukocyte Transendothelial Migration Pathway antibodies	Leukocyte Transendothelial Migration Pathway Diagram

Diseases associated with anti-CD99 antibody

Disease Name	Pubmed Publications
Skin Diseases Antibodies	>97 publications with CD99 and Skin Diseases
Necrosis Antibodies	>97 publications with CD99 and Necrosis
Adenocarcinoma Antibodies	>74 publications with CD99 and Adenocarcinoma
Kidney Diseases Antibodies	>73 publications with CD99 and Kidney Diseases
Neoplasm Metastasis Antibodies	>67 publications with CD99 and Neoplasm Metastasis
Brain Diseases Antibodies	>66 publications with CD99 and Brain Diseases
Ovarian Diseases Antibodies	>66 publications with CD99 and Ovarian Diseases
Liver Neoplasms Antibodies	>36 publications with CD99 and Liver Neoplasms
Hemorrhage Antibodies	>31 publications with CD99 and Hemorrhage
Inflammation Antibodies	>29 publications with CD99 and Inflammation

Organs/Tissues associated with anti-CD99 antibody

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>241 publications with CD99 and Bone
Muscle Antibodies	>197 publications with CD99 and Muscle
Blood Antibodies	>182 publications with CD99 and Blood
Vascular Antibodies	>111 publications with CD99 and Vascular
Skin Antibodies	>97 publications with CD99 and Skin
Lung Antibodies	>97 publications with CD99 and Lung
Kidney Antibodies	>88 publications with CD99 and Kidney
Lymph Node Antibodies	>81 publications with CD99 and Lymph Node
Brain Antibodies	>72 publications with CD99 and Brain
Nerve Antibodies	>62 publications with CD99 and Nerve