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CD146 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS210850

Mouse anti Human CD146 antibody, clone OJ79c recognizes human Cell surface glycoprotein MUC18, also known as CD146, Cell surface glycoprotein P1H12, Melanoma cell adhesion molecule or S-endo 1 endothelial-associated antigen. CD146 is a 646 amino acid single… More details

$560 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD146, Monoclonal Antibody
Full Product Name
MOUSE ANTI HUMAN CD146:FITC
Product Gene Name
anti-CD146 antibody
Clonality
Monoclonal
Isotype
IgG1
Clone Number
OJ79c
Host
Mouse
Form/Format
FITC
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Concentration
IgG concentration 0.1 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Molecular Weight
57,605 Da
OMIM
155735
3D Structure
ModBase 3D Structure for P43121
Perservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
Preparation||Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Target Species
Human
Applications Tested/Suitable for anti-CD146 antibody
Flow cytometry (FC/FACS)
Application Notes for anti-CD146 antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10

Testing Data of anti-CD146 antibody

Published customer image; Conjugated and unconjugated anti-CD326, anti-CD146 and anti-CD104 were analysed by flow cytometry for binding to CORL23 and LoVo cells [black line, negative control; blue line, unconjugated antibody; red line, immunoconjugates with porphyrin 1 (a) or porphyrin 2 (b)].From: Smith K, Malatesti N, Cauchon N, Hunting D, Lecomte R, van Lier JE, Greenman J, Boyle RW. Mono- and tri-cationic porphyrin-monoclonal antibody conjugates: photodynamic activity and mechanism of action. Immunology. 2011 Feb;132(2):256-65.
Mouse Anti-CD146 Monoclonal Antibody – Cat# MBS210850

Testing Data of anti-CD146 antibody

Published customer image: Brain-derived cell lines express markers for mesenchymal stem cells and pericytes but not for glial or neuronal precursors. (A) Representative histogram of flow cytometry analysis of cortical line and (B) ventricular zone line. Progenitors from all donors and both regions highly express MSC (CD90, CD73, CD105, CD29, CD166 and CD49d) and pericyte markers (CD140b/PDGFR-beta, RGS5, CD146, Nestin, a-SMA and NG2). They do not express hematopoietic (CD45), endothelial (CD31, CD34), microglial (CD14, CD11), glial or neuronal precursor cell markers (GFAP, O4) or myofibroblast markers (CD56), (green = isotype, red = respective marker).From: Paul G, Özen I, Christophersen NS, Reinbothe T, Bengzon J, et al. (2012) The Adult Human Brain Harbors Multipotent Perivascular Mesenchymal Stem Cells. PLoS ONE 7(4): e35577.
Mouse Anti-CD146 Monoclonal Antibody, Testing Data – Cat# MBS210850

Other Notes

Small volumes of anti-CD146 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD146 purchase

MBS210850 is an antibody targeting CD146. Also known as MOUSE ANTI HUMAN CD146:FITC. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD146. It may not necessarily be applicable to this product.

NCBI GI #
71274107
NCBI GeneID
4162
NCBI Accession #
NP_006491.2
UniProt Primary Accession #
P43121
UniProt Secondary Accession #
O95812; Q59E86; Q6PHR3; Q6ZTR2
UniProt Related Accession #
P43121
GenBank Nucleotide #
NM_006500.2
NCBI Official Full Name
cell surface glycoprotein MUC18
NCBI Official Synonym Full Names
melanoma cell adhesion molecule
NCBI Official Symbol
MCAM
NCBI Official Synonym Symbols
CD146; MUC18
UniProt Protein Name
Cell surface glycoprotein MUC18
UniProt Synonym Protein Names
Cell surface glycoprotein P1H12; Melanoma cell adhesion molecule; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; S-endo 1 endothelial-associated antigen; CD_antigen: CD146
Protein Family
Cell surface glycoprotein
UniProt Gene Name
MCAM
UniProt Synonym Gene Names
MUC18
UniProt Entry Name
MUC18_HUMAN
NCBI Protein Information
cell surface glycoprotein MUC18; Gicerin; S-endo 1 endothelial-associated antigen; cell surface glycoprotein P1H12; melanoma adhesion molecule; melanoma-associated antigen A32; melanoma-associated antigen MUC18

UniProt Comments for CD146

MCAM: Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: extracellular space; focal adhesion; plasma membrane; integral to membrane; nucleus; external side of plasma membrane

Biological Process: anatomical structure morphogenesis; glomerular filtration; angiogenesis; cell adhesion; positive regulation of cell migration
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Research Articles on CD146

1. With the ability of migration and survival in the advanced osteoarthritis cartilage, CD146+ chondroprogenitors might be "tissue-specific" for cartilage tissue regeneration.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD146 antibody

 Reference Product  PubMed Publications
 CD34 antibody  >23 publications with CD146 and CD34
 PROM1 antibody  >2 publications with CD146 and PROM1
 FYN antibody  >2 publications with CD146 and FYN
 SELE antibody  >1 publications with CD146 and SELE
 EDN1 antibody  >1 publications with CD146 and EDN1

Diseases associated with anti-CD146 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >34 publications with CD146 and Inflammation
 Lung Diseases Antibodies  >29 publications with CD146 and Lung Diseases
 Neoplasms, Experimental Antibodies  >24 publications with CD146 and Neoplasms, Experimental
 Cardiovascular Diseases Antibodies  >23 publications with CD146 and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >22 publications with CD146 and Disease Models, Animal
 Adenocarcinoma Antibodies  >22 publications with CD146 and Adenocarcinoma
 Lung Neoplasms Antibodies  >20 publications with CD146 and Lung Neoplasms
 Breast Neoplasms Antibodies  >19 publications with CD146 and Breast Neoplasms
 Prostatic Neoplasms Antibodies  >16 publications with CD146 and Prostatic Neoplasms
 Nervous System Diseases Antibodies  >16 publications with CD146 and Nervous System Diseases

Organs/Tissues associated with anti-CD146 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >125 publications with CD146 and Blood
 Vascular Antibodies  >101 publications with CD146 and Vascular
 Bone Antibodies  >49 publications with CD146 and Bone
 Skin Antibodies  >44 publications with CD146 and Skin
 Lung Antibodies  >43 publications with CD146 and Lung
 Embryonic Tissue Antibodies  >36 publications with CD146 and Embryonic Tissue
 Bone Marrow Antibodies  >34 publications with CD146 and Bone Marrow
 Muscle Antibodies  >30 publications with CD146 and Muscle
 Prostate Antibodies  >19 publications with CD146 and Prostate
 Brain Antibodies  >18 publications with CD146 and Brain
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