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CD22 Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS210869

MBS210869 is an antibody targeting CD22. Also known as RAT ANTI MOUSE CD22:FITC. Reactive in Mouse. Host: Rat. More details

$510 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
PDF
Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD22, Monoclonal Antibody
Full Product Name
RAT ANTI MOUSE CD22:FITC
Product Gene Name
Clonality
Monoclonal
Isotype
IgG1
Clone Number
OX-97
Host
Rat
Species Reactivity
Mouse
Form/Format
FITC (Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid)
Concentration
IgG concentration 0.1 mg/ml (lot specific)
Specificity
CD22
This item recognises domain 2 of the murine cell surface glycoprotein CD22, which is a member of the sialoadhesin family. CD22 is expressed on a subpopulation of mature B-lymphocytes and may modulate signalling through the B-cell antigen receptor.
Preparation and Storage
Store at +4 degree C or at -20 degree C if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of dispatch.
Molecular Weight
96,582 Da
Chromosome Location
Chromosome: 7; NC_000073.6 (30865404..30880342, complement). Location: 7 B1; 7 19.26 cM
3D Structure
ModBase 3D Structure for P35329
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Preservative Stabilizers
0.09% Sodium Azide
1% Bovine Serum Albumin
Buffer Solution
Phosphate buffered saline
Applications Tested/Suitable for anti-CD22 antibody
Flow Cytometry (FACS)
Application Notes for anti-CD22 antibody
Flow Cytometry: Min Dilution: Neat
Max Dilution: 1/10
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR

Testing Data #1 of anti-CD22 antibody

Staining of mouse spleen cells with Rat anti Mouse CD22:FITC (MBS210869)
Rat Anti-CD22 Monoclonal Antibody – Cat# MBS210869

Testing Data #2 of anti-CD22 antibody

Staining of mouse spleen with Rat anti Mouse CD22: Alexa FluorR 647 (MBS212553)
Rat Anti-CD22 Monoclonal Antibody, Testing Data #2 – Cat# MBS210869

Other Notes

Small volumes of anti-CD22 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CD22. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
B-cell receptor CD22
NCBI Official Synonym Full Names
CD22 antigen
NCBI Official Symbol
Cd22
NCBI Official Synonym Symbols
Lyb8; Lyb-8; A530093D23
UniProt Protein Name
B-cell receptor CD22
UniProt Synonym Protein Names
B-lymphocyte cell adhesion molecule; BL-CAM; Sialic acid-binding Ig-like lectin 2; Siglec-2; T-cell surface antigen Leu-14
Protein Family
UniProt Gene Name
Cd22
UniProt Synonym Gene Names
Lyb-8; Siglec2; BL-CAM; Siglec-2
UniProt Entry Name
CD22_MOUSE
NCBI Protein Information
B-cell receptor CD22; BL-CAM; siglec-2; T-cell surface antigen Leu-14; B-lymphocyte cell adhesion molecule; sialic acid-binding Ig-like lectin 2

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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