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CD49e Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS210929

Rat anti Mouse CD49e antibody, clone MFR5 (5H10) recognizes murine integrin alpha 5 (CD49e), which associates with the beta 1 integrin (CD29) to form the fibronectin receptor CD49e/CD29 (VLA-5). VLA-5 plays an important role in cell adhesion and migration and… More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Product Name
CD49e, Monoclonal Antibody
Full Product Name
RAT ANTI MOUSE CD49e:FITC
Product Gene Name
anti-CD49e antibody
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
MFR5 (5H10)
Host
Rat
Form/Format
FITC
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Concentration
IgG concentration 0.1 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Molecular Weight
115,043 Da
3D Structure
ModBase 3D Structure for P11688
Perservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
Preparation||Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Immunogen
Mouse MC/9 cell line
Fusion Partners||Spleen cells from immunised Lewis rats were fused with cells of the Murine P3X63Ag8.653
Buffer Solution||Phosphate buffered saline
Target Species
Mouse
Applications Tested/Suitable for anti-CD49e antibody
Flow cytometry (FC/FACS)
Application Notes for anti-CD49e antibody
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ml. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR.
Flow Cytometry: Maximum Dilution: Neat

Testing Data of anti-CD49e antibody

Staining of mouse peritoneal macrophages with Rat anti Mouse CD49e : Alexa Fluor 488 (MBS212668A488)
Rat Anti-CD49e Monoclonal Antibody – Cat# MBS210929

Testing Data of anti-CD49e antibody

Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD49e: FITC (MBS210930)
Rat Anti-CD49e Monoclonal Antibody, Testing Data – Cat# MBS210929

Testing Data of anti-CD49e antibody

Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD49e: FITC
Rat Anti-CD49e Monoclonal Antibody, Testing Data – Cat# MBS210929

Testing Data of anti-CD49e antibody

Staining of mouse peritoneal macrophage with Rat anti Mouse CD49e : Alexa Fluor 647 (MBS212668A647)
Rat Anti-CD49e Monoclonal Antibody, Testing Data – Cat# MBS210929

Testing Data of anti-CD49e antibody

Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD49e (MBS212669)
Rat Anti-CD49e Monoclonal Antibody, Testing Data – Cat# MBS210929

Other Notes

Small volumes of anti-CD49e antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD49e purchase

MBS210929 is an antibody targeting CD49e. Also known as RAT ANTI MOUSE CD49e:FITC. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for CD49e. It may not necessarily be applicable to this product.

NCBI GI #
225903442
NCBI GeneID
16402
NCBI Accession #
NP_034707.3
UniProt Primary Accession #
P11688
UniProt Secondary Accession #
E9QN40
UniProt Related Accession #
P11688
GenBank Nucleotide #
NM_010577.3
NCBI Official Full Name
integrin alpha-5 preproprotein
NCBI Official Synonym Full Names
integrin alpha 5 (fibronectin receptor alpha)
NCBI Official Symbol
Itga5
NCBI Official Synonym Symbols
Fnra; VLA5; Cd49e
UniProt Protein Name
Integrin alpha-5
UniProt Synonym Protein Names
CD49 antigen-like family member E; Fibronectin receptor subunit alpha; Integrin alpha-F; VLA-5; CD_antigen: CD49eCleaved into the following 2 chains:Integrin alpha-5 heavy chain; Integrin alpha-5 light chain
Protein Family
Integrin
UniProt Gene Name
Itga5
UniProt Entry Name
ITA5_MOUSE
NCBI Protein Information
integrin alpha-5; CD49 antigen-like family member E; fibronectin receptor alpha polypeptide; fibronectin receptor subunit alpha; integrin alpha-F

UniProt Comments for CD49e

ITGA5: Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-5 associates with beta-1. Interacts with HPS5 and NISCH. Interacts with RAB21 and COMP. Interacts with HIV- 1 Tat. Belongs to the integrin alpha chain family.

Protein type: Membrane protein, integral; Cell adhesion; Motility/polarity/chemotaxis

Cellular Component: Golgi apparatus; cell surface; focal adhesion; endoplasmic reticulum; integral to membrane; intercellular junction; membrane; cytoplasm; plasma membrane; synapse; cytoplasmic vesicle; integrin complex; external side of plasma membrane

Molecular Function: integrin binding; protein binding; metal ion binding; cell adhesion molecule binding; epidermal growth factor receptor binding

Biological Process: heterophilic cell adhesion; integrin-mediated signaling pathway; heterotypic cell-cell adhesion; leukocyte adhesion; positive regulation of peptidyl-tyrosine phosphorylation; cell-cell adhesion mediated by integrin; cell-substrate junction assembly; cell adhesion; cell-substrate adhesion; memory; positive regulation of cell migration
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Research Articles on CD49e

1. Integrin-alpha5beta1 is not required for mural cell functions during development of blood vessels but is required for lymphatic-blood vessel separation and lymphovenous valve formation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD49e antibody

 Reference Product  PubMed Publications
 Itgb1 antibody  >11 publications with CD49e and Itgb1
 Itgb3 antibody  >8 publications with CD49e and Itgb3
 Fn1 antibody  >8 publications with CD49e and Fn1
 Spp1 antibody  >4 publications with CD49e and Spp1
 Itgb5 antibody  >4 publications with CD49e and Itgb5
 Vtn antibody  >3 publications with CD49e and Vtn
 Itgb4 antibody  >1 publications with CD49e and Itgb4
 Tnc antibody  >1 publications with CD49e and Tnc
 Tln1 antibody  >1 publications with CD49e and Tln1

Pathways associated with anti-CD49e antibody

 Products by Pathway  Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Dilated Cardiomyopathy Pathway antibodies  Dilated Cardiomyopathy Pathway Diagram
 Dilated Cardiomyopathy Pathway antibodies  Dilated Cardiomyopathy Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram

Diseases associated with anti-CD49e antibody

 Disease Name  Pubmed Publications
 Neoplasm Invasiveness Antibodies  >9 publications with CD49e and Neoplasm Invasiveness
 Carcinoma Antibodies  >8 publications with CD49e and Carcinoma
 Neoplasm Metastasis Antibodies  >8 publications with CD49e and Neoplasm Metastasis
 Breast Neoplasms Antibodies  >7 publications with CD49e and Breast Neoplasms
 Inflammation Antibodies  >6 publications with CD49e and Inflammation
 Nervous System Diseases Antibodies  >4 publications with CD49e and Nervous System Diseases
 Lung Diseases Antibodies  >3 publications with CD49e and Lung Diseases
 Cardiovascular Diseases Antibodies  >2 publications with CD49e and Cardiovascular Diseases
 Carcinoma, Hepatocellular Antibodies  >2 publications with CD49e and Carcinoma, Hepatocellular
 Neoplasms, Experimental Antibodies  >2 publications with CD49e and Neoplasms, Experimental

Organs/Tissues associated with anti-CD49e antibody

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >12 publications with CD49e and Bone
 Muscle Antibodies  >8 publications with CD49e and Muscle
 Embryonic Tissue Antibodies  >8 publications with CD49e and Embryonic Tissue
 Uterus Antibodies  >7 publications with CD49e and Uterus
 Bone Marrow Antibodies  >7 publications with CD49e and Bone Marrow
 Lung Antibodies  >6 publications with CD49e and Lung
 Liver Antibodies  >5 publications with CD49e and Liver
 Brain Antibodies  >4 publications with CD49e and Brain
 Kidney Antibodies  >2 publications with CD49e and Kidney
 Thymus Antibodies  >1 publications with CD49e and Thymus
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