HISTIDINE Antibody – Mouse Monoclonal

Monoclonal

IgG1

AD1.1.10

Mouse

HRP; Phosphate buffered saline; Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Approx. Protein Concentrations: IgG concentration 1.0 mg/ml (lot specific)

HISTIDINE TAG

Mouse anti Histidine tag antibody, clone AD1.1.10 recognizes proteins and peptides containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine- tagged proteins expressed in mammalian (Hoffmann et al. 2007) and Hwang et al. 2008) and non-mammalian (Zheng et al. 2007; Gunnarsen et al. 2010; and de Vooght et al. 2012) cell lines.

In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells.

This antibody is routinely tested in Western blotting on histidine tagged recombinant proteins and reacts against all histidine-tagged proteins so far tested

It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C. Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

Synthetic Peptide

0.0095% MIT

PAX6 transcription factor linked to histidine tag.

Spleen cells from immunized Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.