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ALPHA Fetoprotein (AFP) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS211649

MBS211649 recognises alpha fetoprotein (AFP), a member of the albuminoid superfamily. It is a 68kD plasma protein found in fetuses over four weeks old, produced by the yolk sac and the fetal liver, with the highest concentration reached during the 12th to… More details

$950 / 1 mg +1 FREE 8GB USB
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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
ALPHA FETOPROTEIN (AFP), Monoclonal Antibody
Full Product Name
MOUSE ANTI HUMAN ALPHA FETOPROTEIN
Product Gene Name
anti-AFP antibody
Clonality
Monoclonal
Isotype
IgG
Clone Number
2I9/2
Host
Mouse
Form/Format
Purified
Ig fraction - liquid
Concentration
IgG concentration 1.0 mg/ml (lot specific)
Specificity
This item recognises alpha fetoprotein (AFP), a member of the albuminoid superfamily. It is a 68kD plasma protein found in fetuses over four weeks old, produced by the yolk sac and the fetal liver, with the highest concentration reached during the 12th to 16th week of gestation. It binds and transports various ligands such as bilirubin, fatty acids, steroids, heavy metals and various drugs. After birth, plasma levels drop to trace amounts in healthy adults.

Elevated AFP levels are indicative of certain cancers, particularly hepatocellular carcinoma, cancer of the testes or ovaries or a malignant teratoma. During pregnancy increased levels of AFP may indicate neural tube defects such as spina bifida, while decreased levels could indicate Down's syndrome.

This item does not cross-react with human albumin and other human serum proteins.
Preparation and Storage
Store at 4°C or at -20°C if preferred.

Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Shelf Life: 18 months from date of despatch.
OMIM
104150
3D Structure
ModBase 3D Structure for P02771
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
Preparation
Ig fraction prepared by chromatography
Immunogen
Human fetal cord serum
Buffer Solution
Phosphate buffered saline
Target Species
Human
Applications Tested/Suitable for anti-AFP antibody
ELISA (EIA)

Testing Data of anti-AFP antibody

Sandwich ELISA analysis of alpha fetoprotein binding using Mouse anti Human alpha fetoprotein as a capture reagent and biotinylated Mouse anti Human alpha fetoprotein as a detection reagent with human alpha fetoprotein as antigen for the generation of a standard curve. Detection is by HRP conjugated Streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . Serum (green) and plasma (orange) samples are both displayed at 1:2 dilution.
Mouse Anti-AFP Monoclonal Antibody – Cat# MBS211649

Testing Data of anti-AFP antibody

Sandwich ELISA analysis of alpha fetoprotein binding using Mouse anti Human alpha fetoprotein as a capture reagent and biotinylated Mouse anti Human alpha fetoprotein (MBS211649) as a detection reagent with human alpha fetoprotein as antigen for the generation of a standard curve. Detection is by HRP conjugated Streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . Serum (green) and plasma (orange) samples are both displayed at 1:2 dilution.
Mouse Anti-AFP Monoclonal Antibody, Testing Data – Cat# MBS211649

Testing Data of anti-AFP antibody

Sandwich ELISA analysis of alpha fetoprotein binding using Mouse anti Human alpha fetoprotein as a capture reagent and biotinylated Mouse anti Human alpha fetoprotein (MBS211395) as a detection reagent with human alpha fetoprotein as antigen for the generation of a standard curve. Detection is by HRP conjugated Streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . Serum (green) and plasma (orange) samples are both displayed at 1:2 dilution.
Mouse Anti-AFP Monoclonal Antibody, Testing Data – Cat# MBS211649

Other Notes

Small volumes of anti-AFP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AFP purchase

MBS211649 is an antibody targeting AFP. Also known as MOUSE ANTI HUMAN ALPHA FETOPROTEIN. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for AFP. It may not necessarily be applicable to this product.

NCBI GI #
4501989
NCBI GeneID
174
NCBI Accession #
NP_001125.1
UniProt Primary Accession #
P02771
UniProt Secondary Accession #
B2RBU3
UniProt Related Accession #
P02771
GenBank Nucleotide #
NM_001134.2
NCBI Official Full Name
alpha-fetoprotein
NCBI Official Synonym Full Names
alpha-fetoprotein
NCBI Official Symbol
AFP
NCBI Official Synonym Symbols
AFPD; FETA; HPAFP
UniProt Protein Name
Alpha-fetoprotein
UniProt Synonym Protein Names
Alpha-1-fetoprotein; Alpha-fetoglobulin
Protein Family
Alpha-fetoprotein
UniProt Gene Name
AFP
UniProt Synonym Gene Names
HPAFP
UniProt Entry Name
FETA_HUMAN
NCBI Protein Information
alpha-fetoprotein; alpha-1-fetoprotein; alpha-fetoglobulin

NCBI Summary for AFP

This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008]
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UniProt Comments for AFP

AFP: Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. Dimeric and trimeric forms have been found in addition to the monomeric form. Plasma. Synthesized by the fetal liver and yolk sac. Belongs to the ALB/AFP/VDB family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: extracellular space; cytoplasm

Molecular Function: metal ion binding

Biological Process: transport; progesterone metabolic process; ovulation from ovarian follicle

Disease: Alpha-fetoprotein Deficiency; Alpha-fetoprotein, Hereditary Persistence Of
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Research Articles on AFP

1. The prognosis of patients undergoing living donor liver transplantation for hepatocellular carcinoma strongly relies on maximum AFP or des-gamma-carboxyprothrombin values before transplantation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-AFP antibody

 Reference Product  PubMed Publications
 JUN antibody  >936 publications with AFP and JUN
 GPC3 antibody  >69 publications with AFP and GPC3
 IGFBP1 antibody  >19 publications with AFP and IGFBP1
 FOXA1 antibody  >12 publications with AFP and FOXA1
 TP53 antibody  >8 publications with AFP and TP53
 FOXA3 antibody  >3 publications with AFP and FOXA3
 AHSG antibody  >1 publications with AFP and AHSG

Pathways associated with anti-AFP antibody

 Products by Pathway  Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
 FOXA2 And FOXA3 Transcription Factor Networks Pathway antibodies  FOXA2 And FOXA3 Transcription Factor Networks Pathway Diagram
 Glucocorticoid Receptor Regulatory Network Pathway antibodies  Glucocorticoid Receptor Regulatory Network Pathway Diagram
 Hippo Signaling Pathway antibodies  Hippo Signaling Pathway Diagram
 Hippo Signaling Pathway antibodies  Hippo Signaling Pathway Diagram

Diseases associated with anti-AFP antibody

 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >4009 publications with AFP and Liver Diseases
 Liver Neoplasms Antibodies  >3894 publications with AFP and Liver Neoplasms
 Carcinoma, Hepatocellular Antibodies  >3666 publications with AFP and Carcinoma, Hepatocellular
 Nervous System Diseases Antibodies  >1267 publications with AFP and Nervous System Diseases
 Liver Cirrhosis Antibodies  >1122 publications with AFP and Liver Cirrhosis
 Neoplasm Metastasis Antibodies  >544 publications with AFP and Neoplasm Metastasis
 Neoplasms, Experimental Antibodies  >438 publications with AFP and Neoplasms, Experimental
 Liver Neoplasms, Experimental Antibodies  >374 publications with AFP and Liver Neoplasms, Experimental
 Lung Neoplasms Antibodies  >345 publications with AFP and Lung Neoplasms
 Stomach Neoplasms Antibodies  >331 publications with AFP and Stomach Neoplasms

Organs/Tissues associated with anti-AFP antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >4873 publications with AFP and Liver
 Blood Antibodies  >3691 publications with AFP and Blood
 Vascular Antibodies  >587 publications with AFP and Vascular
 Lung Antibodies  >393 publications with AFP and Lung
 Brain Antibodies  >327 publications with AFP and Brain
 Embryonic Tissue Antibodies  >290 publications with AFP and Embryonic Tissue
 Muscle Antibodies  >211 publications with AFP and Muscle
 Kidney Antibodies  >175 publications with AFP and Kidney
 Placenta Antibodies  >164 publications with AFP and Placenta
 Uterus Antibodies  >101 publications with AFP and Uterus
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