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Na-Cl Cotransporter (NCCT) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2116619
SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment

MBS2116619 is an antibody targeting NCCT. Also known as APC-Cy7 Linked Monoclonal Antibody to Na-Cl Cotransporter (NCCT). Reactive in Human, Pig. Host: Mouse. More details

$475 / 0.1 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Na-Cl Cotransporter (NCCT), Monoclonal Antibody
Full Product Name
APC-Cy7 Linked Monoclonal Antibody to Na-Cl Cotransporter (NCCT)
Product Gene Name
Product Synonym Names
SLC12A3; TSC; NCC; Solute Carrier Family 12 Member 3,Sodium/Chloride Transporters; Thiazide-sensitive sodium-chloride cotransporter; Na-Cl symporter
Immunogen Sequence
Glu3~Cys14 6
Clonality
Monoclonal
Isotype
IgG2b Kappa
Clone Number
C2
Host
Mouse
Species Reactivity
Human, Pig
Form/Format
Liquid; 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Purity/Purification
Protein A + Protein G Affinity Chromatography
Concentration
>=100ug/ml (lot specific)
Specificity
The antibody is a mouse monoclonal antibody raised against NCCT. It has been selected for its ability to recognize NCCT in immunohistochemical staining and western blotting.
Preparation and Storage
Store at 4 degree C for frequent use. Aliquot and store at -20 degree C for 12 months.
Avoid repeated freeze/thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Matching Pairs
Unconjugated Antibody: NCCT Clone #C2 (MBS2012530)
APC-CY7 Conjugated Antibody: NCCT Clone #C2 (MBS2116619)
Matching Pairs
APC-CY7 Conjugated Antibody: NCCT Clone #C2 (MBS2116619)
Immunogen: NCCT (MBS2011910)
Molecular Weight
113,808 Da
OMIM
600968
3D Structure
ModBase 3D Structure for P55017
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Immunogen Sequence
Glu3~Cys146
Cross Reactivity
Human
Conjugation
APC-Cy7

Product Categories/Family for anti-NCCT antibody

ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-NCCT antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Other Notes

Small volumes of anti-NCCT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NCCT. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Solute carrier family 12 member 3
NCBI Official Synonym Full Names
solute carrier family 12 (sodium/chloride transporter), member 3
NCBI Official Symbol
SLC12A3
NCBI Official Synonym Symbols
NCC; TSC; NCCT
UniProt Protein Name
Solute carrier family 12 member 3
UniProt Synonym Protein Names
Na-Cl cotransporter; NCC; Na-Cl symporter; Thiazide-sensitive sodium-chloride cotransporter
UniProt Gene Name
SLC12A3
UniProt Synonym Gene Names
NCC; TSC; NCC
UniProt Entry Name
S12A3_HUMAN
NCBI Protein Information
solute carrier family 12 member 3; Na-Cl symporter; Na-Cl cotransporter; thiazide-sensitive Na-Cl cotransporter; thiazide-sensitive sodium-chloride cotransporter; NaCl electroneutral thiazide-sensitive cotransporter

NCBI Summary for NCCT

This gene encodes a renal thiazide-sensitive sodium-chloride cotransporter that is important for electrolyte homeostasis. This cotransporter mediates sodium and chloride reabsorption in the distal convoluted tubule. Mutations in this gene cause Gitelman syndrome, a disease similar to Bartter's syndrome, that is characterized by hypokalemic alkalosis combined with hypomagnesemia, low urinary calcium, and increased renin activity associated with normal blood pressure. This cotransporter is the target for thiazide diuretics that are used for treating high blood pressure. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for NCCT

NCCT: Key mediator of sodium and chloride reabsorption in this nephron segment, accounting for a significant fraction of renal sodium reabsorption. Interacts with KLHL3. Predominant in kidney. Activated by WNK3. Belongs to the SLC12A transporter family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter, SLC family; Membrane protein, integral; Transporter; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 16q13

Cellular Component: membrane; integral to plasma membrane; apical plasma membrane; plasma membrane; cytosol

Molecular Function: protein binding; transporter activity; sodium:chloride symporter activity

Biological Process: transport; sodium ion transport; chloride transport; ion transport; transmembrane transport

Disease: Gitelman Syndrome
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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