Mouse Anti-GM-CSF Monoclonal Antibody – Cat# MBS211665

GM-CSF Antibody – Mouse Monoclonal

Name: GM-CSF Antibody; Monoclonal; Mouse; Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS211665
Price: 390 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS211665

MBS211665 recognises recombinant and native human granulocyte macrophage colony stimulating factor (GM-CSF). Human Granulocyte colony stimulating factor (GM-CSF) is a 14.6kDa glycoprotein, which is produced by T cells, macrophages, fibroblasts and endothelial… More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

GM-CSF, Monoclonal Antibody

Full Product Name

MOUSE ANTI HUMAN GM-CSF

Product Gene Name

anti-GM-CSF antibody

Clonality

Monoclonal

Isotype

IgG1

Clone Number

3209 (30-4)

Host

Mouse

Species Reactivity

Mouse (Weak)

Form/Format

Purified
Purified IgG - liquid

Concentration

IgG concentration 1.0 mg/ml (lot specific)

Specificity

This item recognises recombinant and native human granulocyte macrophage colony stimulating factor (GM-CSF). Human Granulocyte colony stimulating factor (GM-CSF) is a 14.6kDa glycoprotein, which is produced by T cells, macrophages, fibroblasts and endothelial cells. GM-CSF is involved in the stimulation of proliferation and differentiation of granulocyte and macrophage progenitor cells.The neutralisation dose (ND50) of this batch, as determined by the proliferation of TF-1 cells, is approximately 0.3-0.5 ug/ml in the presence of 0.5 ng/ml of recombinant human GM-CSF. The ND50 may vary depending on conditions.

Preparation and Storage

Store at -20 degree C only. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Molecular Weight

16,295 Da

OMIM

138960

3D Structure

ModBase 3D Structure for P04141

Perservative Stabilisers

1% trehalose
Preparation||Purified IgG prepared by affinity chromatography on Protein A.

Immunogen

Recombinant protein corresponding to amino acids 14 to 144 of human GM-CSF produced in E Coli.
Buffer Solution||Phosphate buffered saline

Target Species

Human

Applications Tested/Suitable for anti-GM-CSF antibody

Immunohistology Frozen, ELISA (EIA), Functional Assays (FN), Western Blot (WB)*

Application Notes for anti-GM-CSF antibody

Immunohistology - Frozen: Minimum Dilution: 8.0; Maximum Dilution: 25 ug/ml;
ELISA: Minimum Dilution: 0.5; Maximum Dilution: 1.0 ug/ml;
Functional Assays: Minimum Dilution: 0.3; Maximum Dilution: 0.5 ug/ml;
Western Blotting: Minimum Dilution: 1.0; Maximum Dilution: 2.0 ug/ml; Application Note: 4740-0955 should be used under non-reducing conditions, reducing conditions are not recommended.

Other Notes

Small volumes of anti-GM-CSF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GM-CSF purchase

MBS211665 is an antibody targeting GM-CSF. Also known as MOUSE ANTI HUMAN GM-CSF. Reactive in Mouse (Weak). Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for GM-CSF. It may not necessarily be applicable to this product.

NCBI GI #

27437030

NCBI GeneID

1437

NCBI Accession #

NP_000749.2

UniProt Primary Accession #

P04141

UniProt Secondary Accession #

Q14CE8; Q2VPI8; Q8NFI6

UniProt Related Accession #

P04141

GenBank Nucleotide #

NM_000758.3

NCBI Official Full Name

granulocyte-macrophage colony-stimulating factor

NCBI Official Synonym Full Names

colony stimulating factor 2 (granulocyte-macrophage)

NCBI Official Symbol

CSF2

NCBI Official Synonym Symbols

GMCSF

UniProt Protein Name

Granulocyte-macrophage colony-stimulating factor

UniProt Synonym Protein Names

Colony-stimulating factor; CSF; Molgramostin; Sargramostim

Protein Family

Granulocyte colony-stimulating factor receptor

UniProt Gene Name

CSF2

UniProt Synonym Gene Names

GMCSF; GM-CSF; CSF

UniProt Entry Name

CSF2_HUMAN

NCBI Protein Information

granulocyte-macrophage colony-stimulating factor; CSF; granulocyte macrophage-colony stimulating factor; molgramostin; sargramostim

NCBI Summary for GM-CSF

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq, Jul 2008]

UniProt Comments for GM-CSF

GMCSF: Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Belongs to the GM-CSF family.

Protein type: Secreted; Cytokine; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; growth factor activity; granulocyte macrophage colony-stimulating factor receptor binding; cytokine activity

Biological Process: negative regulation of cytolysis; epithelial fluid transport; embryonic placenta development; macrophage activation; positive regulation of tyrosine phosphorylation of Stat5 protein; positive regulation of interleukin-23 production; positive regulation of cell proliferation; myeloid dendritic cell differentiation; immune response; positive regulation of DNA replication

Research Articles on GM-CSF

1. Treatment with GM-CSF increased the expression of vimentin and ZEB1, similar to TGF-b1 treatment, and decreased the expression of E-cadherin. Our results suggest that GM-CSF could induce EMT in human HERS/ERM cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GM-CSF antibody

Reference Product	PubMed Publications
IL4 antibody	>18 publications with GM-CSF and IL4
IL3 antibody	>14 publications with GM-CSF and IL3
IL5 antibody	>10 publications with GM-CSF and IL5
CSF2RB antibody	>10 publications with GM-CSF and CSF2RB
CSF2RA antibody	>5 publications with GM-CSF and CSF2RA
STAT3 antibody	>3 publications with GM-CSF and STAT3
JAK2 antibody	>2 publications with GM-CSF and JAK2
FOS antibody	>2 publications with GM-CSF and FOS
SDC2 antibody	>1 publications with GM-CSF and SDC2

Pathways associated with anti-GM-CSF antibody

Products by Pathway	Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies	Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
Calcium Signaling In The CD4+ TCR Pathway antibodies	Calcium Signaling In The CD4+ TCR Pathway Diagram
Cytokine Signaling In Immune System Pathway antibodies	Cytokine Signaling In Immune System Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokines And Inflammatory Response Pathway antibodies	Cytokines And Inflammatory Response Pathway Diagram
Fc Epsilon RI Signaling Pathway antibodies	Fc Epsilon RI Signaling Pathway Diagram
Fc Epsilon RI Signaling Pathway antibodies	Fc Epsilon RI Signaling Pathway Diagram

Diseases associated with anti-GM-CSF antibody

Disease Name	Pubmed Publications
Inflammation Antibodies	>41 publications with GM-CSF and Inflammation
Necrosis Antibodies	>26 publications with GM-CSF and Necrosis
Lung Diseases Antibodies	>9 publications with GM-CSF and Lung Diseases
Breast Neoplasms Antibodies	>7 publications with GM-CSF and Breast Neoplasms
Fibrosis Antibodies	>4 publications with GM-CSF and Fibrosis
Neoplasms, Experimental Antibodies	>4 publications with GM-CSF and Neoplasms, Experimental
Carcinoma Antibodies	>3 publications with GM-CSF and Carcinoma
Leukemia, Myeloid, Acute Antibodies	>3 publications with GM-CSF and Leukemia, Myeloid, Acute
Arthritis, Rheumatoid Antibodies	>3 publications with GM-CSF and Arthritis, Rheumatoid
Lung Neoplasms Antibodies	>3 publications with GM-CSF and Lung Neoplasms

Organs/Tissues associated with anti-GM-CSF antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>43 publications with GM-CSF and Blood
Bone Antibodies	>15 publications with GM-CSF and Bone
Lung Antibodies	>12 publications with GM-CSF and Lung
Intestine Antibodies	>2 publications with GM-CSF and Intestine