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NESTIN (NES) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS211830

Mouse anti Rat Nestin antibody, clone Rat-401 recognizes rat nestin, a large intermediate filament protein transiently expressed in embryonic glian cells (Hockfield and McKay 1985). It is predominately expressed in stem cells of the developing nervous system.… More details

$1050 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NESTIN, Monoclonal Antibody
Full Product Name
MOUSE ANTI RAT NESTIN
Product Gene Name
Clonality
Monoclonal
Isotype
IgG1
Clone Number
Rat-401 (4D4)
Host
Mouse
Species Reactivity
Mouse
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 0.5mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Molecular Weight
198,623 Da
3D Structure
ModBase 3D Structure for P21263
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
Buffer Solution
TRIS buffered saline.
Target Species
Rat
Applications Tested/Suitable for anti-Nes antibody
Immunohistology Frozen, Immunofluorescence (IF), Immunohistology Paraffin, Western Blot (WB)
Application Notes for anti-Nes antibody
Western Blot: Mouse anti Rat Nestin antibody, clone Rat-401 reacts with a band at 200-220 kDa in reducing gels of newborn rat or mouse cell extracts.
For western blotting it is recommended that samples should be boiled in 4 volumes of 125 mM Tris, pH 6.8, 10% 2-mercaptoethanol, 10% glycerol and 4.6% SDS. Membranes should be blocked with milk or BSA. 5% PAGE gels are suggested.
Immunohistology: We recommend perfusing tissues with 4% paraformaldehyde at pH 7.4 for light microscopy or with either 4% paraformaldehyde at pH 10.0 or 4% paraformaldehyde with 0.1% glutaraldehyde at pH 7.4 for EM.
For Immunocytochemistry we recommend using cells fixed in 4% paraformaldehyde buffered with 50 mM sodium borate at pH 9.5.
Immunohistology - Frozen: Minimum Dilution: 1/20; Maximum Dilution: 1/200
Immunohistology - Paraffin: Minimum Dilution: 1/20; Maximum Dilution: 1/200

Other Notes

Small volumes of anti-Nes antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Nes purchase

MBS211830 is an antibody targeting Nes. Also known as MOUSE ANTI RAT NESTIN. Reactive in Mouse. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Nes. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
nestin
NCBI Official Synonym Full Names
nestin
NCBI Official Symbol
Nes
UniProt Protein Name
Nestin
Protein Family
UniProt Gene Name
Nes
UniProt Entry Name
NEST_RAT
NCBI Protein Information
nestin

NCBI Summary for Nes

intermediate filament protein specifically expressed in neuroepithelial stem cells; may play a role in neural cell differentiation [RGD, Feb 2006]

UniProt Comments for Nes

Required for brain and eye development (). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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